Phenotypes associated with this allele

• Allele Symbol: Lcp2^tm1.1Wz
• Allele Name: targeted mutation 1.1, Weiguo Zhang
• Allele ID: MGI:3850705
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lcp2^tm1.1Wz/Lcp2^tm1.1Wz	B6.Cg-Lcp2^tm1.1Wz	MGI:3850710
cx2	Grap2^tm1Amch/Grap2^tm1Amch Lcp2^tm1.1Wz/Lcp2^tm1.1Wz	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3850712
cx3	Lcp2^tm1.1Wz/Lcp2^tm1.1Wz Tg(TcraH-Y,TcrbH-Y)71Vbo/0	involves: C57BL/6 * C57BL/6J * DBA/2J	MGI:3850711

Genotype
MGI:3850710

hm1

• Allelic Composition: Lcp2^tm1.1Wz/Lcp2^tm1.1Wz
• Genetic Background: B6.Cg-Lcp2^tm1.1Wz

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:150304 )

N • unlike in Lcp2 null mice, gamma/delta TCR+ thymocyte numbers are normal

decreased T cell proliferation ( J:150304 )

• following stimulation with anti-CD3 and anti-CD28, CD4+CD44^low T cells exhibit decreased proliferation compared to in heterozygous controls

• however, proliferation in response to PMA plus ionomycin is normal

small thymus ( J:150304 )

• at 4 weeks compared with heterozygote controls

abnormal T cell differentiation ( J:150304 )

• mice exhibit a minor developmental block at the DN3 to DN4 checkpoint unlike in heterozygote controls

decreased double-negative T cell number ( J:150304 )

• the percentage of DN4 thymocytes is mildly decreased compared to in heterozygote controls

• however, the absolute number of DN thymocytes is normal

increased double-negative T cell number ( J:150304 )

• the total number of DP thymocytes is moderately decreased compared to in heterozygote controls

abnormal T cell subpopulation ratio ( J:150304 )

• the percentage of double negative thymocytes is slightly increased compared to in heterozygote controls

• the percentage of DN3 thymocytes is moderately increased compared to in heterozygote controls

• the percentage of DP thymocytes is increased compared to in heterozygote controls

• the percentage of CD4+ SP thymocytes is reduced compared to in heterozygote controls

• a relatively small decrease in CD4-CD8+ thymocytes, compared to in heterozygote controls, is observed

decreased T cell number ( J:150304 )

• mice exhibit a minor developmental block at the DN3 to DN4 checkpoint unlike in heterozygote controls

decreased thymocyte number ( J:150304 )

• at 4 weeks compared with heterozygote controls

decreased CD4-positive, alpha-beta T cell number ( J:150304 )

• in the thymus and 50% in the spleen compared to in heterozygote control

• the number of CD4+ cells in the peripheral lymph nodes is decreased compared to in heterozygote controls

decreased CD8-positive, alpha-beta T cell number ( J:150304 )

• the absolute number of CD8+ SP thymocytes and splenocytes is reduced by 50% compared to in heterozygote controls

• the number of CD8+ cells in the peripheral lymph nodes is decreased compared to in heterozygote controls

abnormal effector T cell morphology ( J:150304 )

• the frequency of effector-like CD44^highCD62L^lowCD4+ T cells is increased while the percentage of CD44^lowCD62L^highCD4+ T cells is decreased compared to in heterozygote controls

lymph node hypoplasia ( J:150304 )

decreased interleukin-2 secretion ( J:150304 )

• following stimulation with anti-CD3 and anti-CD28, CD4+ T cells produce less IL2 compared with similarly treated heterozygote cells

hematopoietic system

decreased T cell proliferation ( J:150304 )

• following stimulation with anti-CD3 and anti-CD28, CD4+CD44^low T cells exhibit decreased proliferation compared to in heterozygous controls

• however, proliferation in response to PMA plus ionomycin is normal

small thymus ( J:150304 )

• at 4 weeks compared with heterozygote controls

abnormal T cell differentiation ( J:150304 )

• mice exhibit a minor developmental block at the DN3 to DN4 checkpoint unlike in heterozygote controls

increased double-negative T cell number ( J:150304 )

• the total number of DP thymocytes is moderately decreased compared to in heterozygote controls

abnormal T cell subpopulation ratio ( J:150304 )

• the percentage of double negative thymocytes is slightly increased compared to in heterozygote controls

• the percentage of DN3 thymocytes is moderately increased compared to in heterozygote controls

• the percentage of DP thymocytes is increased compared to in heterozygote controls

• the percentage of CD4+ SP thymocytes is reduced compared to in heterozygote controls

• a relatively small decrease in CD4-CD8+ thymocytes, compared to in heterozygote controls, is observed

decreased T cell number ( J:150304 )

• mice exhibit a minor developmental block at the DN3 to DN4 checkpoint unlike in heterozygote controls

decreased thymocyte number ( J:150304 )

• at 4 weeks compared with heterozygote controls

decreased double-negative T cell number ( J:150304 )

• the percentage of DN4 thymocytes is mildly decreased compared to in heterozygote controls

• however, the absolute number of DN thymocytes is normal

decreased CD4-positive, alpha-beta T cell number ( J:150304 )

• in the thymus and 50% in the spleen compared to in heterozygote control

• the number of CD4+ cells in the peripheral lymph nodes is decreased compared to in heterozygote controls

decreased CD8-positive, alpha-beta T cell number ( J:150304 )

• the absolute number of CD8+ SP thymocytes and splenocytes is reduced by 50% compared to in heterozygote controls

• the number of CD8+ cells in the peripheral lymph nodes is decreased compared to in heterozygote controls

abnormal effector T cell morphology ( J:150304 )

• the frequency of effector-like CD44^highCD62L^lowCD4+ T cells is increased while the percentage of CD44^lowCD62L^highCD4+ T cells is decreased compared to in heterozygote controls

endocrine/exocrine glands

small thymus ( J:150304 )

• at 4 weeks compared with heterozygote controls

decreased thymocyte number ( J:150304 )

• at 4 weeks compared with heterozygote controls

cellular

decreased T cell proliferation ( J:150304 )

• following stimulation with anti-CD3 and anti-CD28, CD4+CD44^low T cells exhibit decreased proliferation compared to in heterozygous controls

• however, proliferation in response to PMA plus ionomycin is normal

Genotype
MGI:3850712

cx2

• Allelic Composition: Grap2^tm1Amch/Grap2^tm1Amch; Lcp2^tm1.1Wz/Lcp2^tm1.1Wz
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

immune system

abnormal T cell differentiation ( J:150304 )

• T cell developmental defects are more severe than in either single homozygote

decreased double-positive T cell number ( J:150304 )

• CD4+CD8+ thymocytes make up only 18% of the total thymocytes

decreased CD4-positive, alpha-beta T cell number ( J:150304 )

decreased CD8-positive, alpha-beta T cell number ( J:150304 )

decreased single-positive T cell number ( J:150304 )

• very few mature T cells are detected in the periphery

hematopoietic system

abnormal T cell differentiation ( J:150304 )

• T cell developmental defects are more severe than in either single homozygote

decreased double-positive T cell number ( J:150304 )

• CD4+CD8+ thymocytes make up only 18% of the total thymocytes

decreased CD4-positive, alpha-beta T cell number ( J:150304 )

decreased CD8-positive, alpha-beta T cell number ( J:150304 )

decreased single-positive T cell number ( J:150304 )

• very few mature T cells are detected in the periphery

Genotype
MGI:3850711

cx3

• Allelic Composition: Lcp2^tm1.1Wz/Lcp2^tm1.1Wz; Tg(TcraH-Y,TcrbH-Y)71Vbo/0
• Genetic Background: involves: C57BL/6 * C57BL/6J * DBA/2J

immune system

abnormal T cell differentiation ( J:150304 )

• thymic negative selection is impaired in male mice

• thymic positive selection is impaired in female mice

hematopoietic system

abnormal T cell differentiation ( J:150304 )

• thymic negative selection is impaired in male mice

• thymic positive selection is impaired in female mice