Phenotypes associated with this allele

• Allele Symbol: Tcirg1^tm1.2Ywd
• Allele Name: targeted mutation 1.2, Yoh Wada
• Allele ID: MGI:3852642
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tcirg1^tm1.2Ywd/Tcirg1^tm1.2Ywd	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N * SJL	MGI:4355583

Genotype
MGI:4355583

hm1

• Allelic Composition: Tcirg1^tm1.2Ywd/Tcirg1^tm1.2Ywd
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

absent teeth ( J:150327 )

• homozygous mutant mice fail to develop teeth

mortality/aging

premature death ( J:150327 )

• mice homozygous for this mutation are born at the expected Mendelian frequency but die within one month

craniofacial

absent teeth ( J:150327 )

• homozygous mutant mice fail to develop teeth

growth/size/body

absent teeth ( J:150327 )

• homozygous mutant mice fail to develop teeth

decreased body size ( J:150327 )

• although indistinguishable from wild-type littermates at birth, by two weeks of age homozygous mutant mice are noticeably smaller

immune system

abnormal macrophage physiology ( J:150327 )

• nascent mutant macrophages accumulate normal steady-state levels of mature forms of lysosomal enzymes and exhibit a normal time course of acquisition and loss of lysosomal markers

• mutant and wild-type macrophages internalize bacteria with equal efficiency, but whereas E. coli ingested by wild-type macrophages become rounded, then fragmented within three hours, those phagocytosed by mutant macrophages retain their rod shape even after a 24-hour chase

• within one hour of macrophages' ingestion of FITC labeled beads, ratiometric analysis of the beads' fluorescence indicates that the phagosomes of wild-type macrophages have become acidic (pH 5.9 +/- 1.0), but phagosomes of mutant macrophages remain at neutral pH (pH 7.0 +/- 0.4)

hematopoietic system

abnormal macrophage physiology ( J:150327 )

• nascent mutant macrophages accumulate normal steady-state levels of mature forms of lysosomal enzymes and exhibit a normal time course of acquisition and loss of lysosomal markers

• mutant and wild-type macrophages internalize bacteria with equal efficiency, but whereas E. coli ingested by wild-type macrophages become rounded, then fragmented within three hours, those phagocytosed by mutant macrophages retain their rod shape even after a 24-hour chase

• within one hour of macrophages' ingestion of FITC labeled beads, ratiometric analysis of the beads' fluorescence indicates that the phagosomes of wild-type macrophages have become acidic (pH 5.9 +/- 1.0), but phagosomes of mutant macrophages remain at neutral pH (pH 7.0 +/- 0.4)