Analysis Tools
mortality/aging
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• mice are born at lower than predicted Mendelian ratios (16% versus expected 25%)
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growth/size/body
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• at 16 weeks of age, males show a significant reduction in body weight that is comparable to that observed in Kpna6tm1Bdr homozygous males
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• male mice exhibit significant postnatal growth retardation, although younger mice are less affected
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reproductive system
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N |
• adult male mice are fertile and display normal relative testis weight and size
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• H&E staining showed aberrant sperm orientation in the seminiferous tubules
• however, sperm transport through the seminiferous epithelium is normal and no residual sperm are detected in stage IX-XII tubules
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• vimentin-based filaments are no longer stretched across the Sertoli cell cytosol but appear to be retracted and wrapped around the cell nuclei, indicating a defect in vimentin distribution
• however, beta-III tubulin organization in Sertoli cells is normal
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• at 12-18 weeks of age, number of Sertoli cells per tubule is significantly lower than in wild-type controls
• however, Sertoli cell numbers are normal at 4 weeks of age
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• aberrant localization of Sertoli cell nuclei is frequently observed in the seminiferous tubules
• immunofluorescence showed a marked reduction of the androgen receptor (AR) in Sertoli cell nuclei
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• immunological blood-testis barrier (BTB) appears to be slightly impaired as antibodies against testicular antigens are occasionally present
• however, no major changes in BTB integrity are detected in a biotin diffusion assay
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• onset of the first wave of spermatogenesis is significantly delayed, consistent with Sertoli cell dysfunction
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• surprisingly, total cauda epididymal sperm count is significantly lower than in wild-type males
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• number of step 1-8 round spermatids is significantly decreased
• however, ratio of round spermatids to pachytene spermatocytes is normal
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• number of leptotene/zygotene spermatocytes is markedly decreased in stages X-XI
• however, no further decrease is seen in the number of stage I-VIII pachytene spermatocytes
• ratio of pachytene to leptotene spermatocytes is normal
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cellular
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• surprisingly, total cauda epididymal sperm count is significantly lower than in wild-type males
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• number of step 1-8 round spermatids is significantly decreased
• however, ratio of round spermatids to pachytene spermatocytes is normal
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• number of leptotene/zygotene spermatocytes is markedly decreased in stages X-XI
• however, no further decrease is seen in the number of stage I-VIII pachytene spermatocytes
• ratio of pachytene to leptotene spermatocytes is normal
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endocrine/exocrine glands
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• H&E staining showed aberrant sperm orientation in the seminiferous tubules
• however, sperm transport through the seminiferous epithelium is normal and no residual sperm are detected in stage IX-XII tubules
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• vimentin-based filaments are no longer stretched across the Sertoli cell cytosol but appear to be retracted and wrapped around the cell nuclei, indicating a defect in vimentin distribution
• however, beta-III tubulin organization in Sertoli cells is normal
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• at 12-18 weeks of age, number of Sertoli cells per tubule is significantly lower than in wild-type controls
• however, Sertoli cell numbers are normal at 4 weeks of age
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• aberrant localization of Sertoli cell nuclei is frequently observed in the seminiferous tubules
• immunofluorescence showed a marked reduction of the androgen receptor (AR) in Sertoli cell nuclei
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• immunological blood-testis barrier (BTB) appears to be slightly impaired as antibodies against testicular antigens are occasionally present
• however, no major changes in BTB integrity are detected in a biotin diffusion assay
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