Analysis Tools
mortality/aging
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• all mice die before E13.5 to E15.5
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• 95% of mice die by E13.5
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endocrine/exocrine glands
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• pancreatic epithelium exhibits less branched structures than in wild-type mice
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• explants of dorsal pancreatic buds from E11.5 mice produce fewer amylase+ cells compared with wild-type explants
• at E11.5, dorsal and ventral pancreatic buds are closely linked unlike in wild-type mice
• at E13.5, dorsal pancreatic buds are smaller than in wild-type mice
• at E15.5, mice exhibit fewer glucagon+ and insulin+ cells and no amylase secreting cells compared to in wild-type mice
• at E15.5, pancreatic epithelial cells are restricted to a progenitor state, as determined by marker expression, unlike in wild-type mice
• in culture, pancreatic epithelium exhibit impaired growth, branching morphogenesis and lineage differentiation unlike wild-type cells
• however, treatment with an Notch signaling inhibitor, DAPT, partially rescues pancreatic epithelial growth and endocrine differentiation
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• at E15.5, mice exhibit fewer glucagon+ cells compared to in wild-type mice
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• explants of dorsal pancreatic buds from E11.5 mice produce fewer insulin+ cells compared with wild-type explants
• at E15.5, mice exhibit fewer insulin+ cells compared to in wild-type mice
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cellular
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• at E15.5, mice exhibit fewer glucagon+ cells compared to in wild-type mice
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• explants of dorsal pancreatic buds from E11.5 mice produce fewer insulin+ cells compared with wild-type explants
• at E15.5, mice exhibit fewer insulin+ cells compared to in wild-type mice
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• in vivo and ex vitro
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• mice exhibit systemic endoplasmic reticulum stress unlike wild-type mice
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liver/biliary system
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• in vivo and ex vitro
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