Phenotypes associated with this allele

• Allele Symbol: Oga^{Gt(YTC085)Byg}
• Allele Name: gene trap YTC085, BayGenomics
• Allele ID: MGI:3883869
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Oga^Gt(YTC085)Byg/Oga^Gt(YTC085)Byg	B6J.129P2-Oga^Gt(YTC085)Byg	MGI:5660641

Genotype
MGI:5660641

hm1

• Allelic Composition: Oga^{Gt(YTC085)Byg}/Oga^{Gt(YTC085)Byg}
• Genetic Background: B6J.129P2-Oga^{Gt(YTC085)Byg}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

perinatal lethality, complete penetrance ( J:218150 )

• homozygotes exhibit perinatal lethality, with no mutants identified at P1

growth/size/body

decreased birth weight ( J:218150 )

• significantly reduced body weight at P0

decreased fetal weight ( J:218150 )

• significantly reduced body weight at E18.5

prenatal growth retardation ( J:218150 )

• at E12.5, E14.5, and E18.5, homozygotes display significantly decreased intrauterine growth relative to controls

• however, no gross anatomical abnormalities are detected at E14.5

respiratory system

atelectasis ( J:218150 )

• whereas control lungs float in water, lungs dissected from mutant newborns are dense and sink

• H&E staining revealed that the alveolar space is markedly reduced at E18.5 and P0

respiratory failure ( J:218150 )

• homozygotes die soon after birth as a result of respiratory failure

cellular

aneuploidy ( J:218150 )

• failure to complete cytokinesis ultimately causes aneuploidy

abnormal mitosis ( J:218150 )

• mutant MEFs display mitotic defects and downregulation of mitotic regulators

• mitotic defects include cytokinesis failure and binucleation, increased lagging chromosomes, and micronuclei formation

abnormal mitotic cytokinesis ( J:218150 )

• 18 of 53 mutant MEFs fail to complete cytokinesis and result in binucleated cells

decreased fibroblast proliferation ( J:218150 )

• MEFs isolated from mid-gestation (13.5 dpc) mutant embryos exhibit reduced growth rates at passages 4 and 7 relative to wild-type MEFs

• mutant MEFs show reduced population-doubling time relative to wild-type MEFs

• reduced growth rate leads to senescence caused by mitotic defects

• decreased cell growth is not due to increased apoptosis, as shown by annexin-V staining

early cellular replicative senescence ( J:218150 )

• mutant MEFs exhibit enlarged and flattened cell morphology, indicative of senescence

chromosomal instability ( J:218150 )

• mutant MEFs fail to maintain genomic stability, followed by abnormal chromosome segregation and cytokinesis failure