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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Slc25a32Gt(IST11480G10)Tigm
gene trap IST11480G10, Texas A&M Institute for Genomic Medicine
MGI:4011881
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Slc25a32Gt(IST11480G10)Tigm/Slc25a32Gt(IST11480G10)Tigm involves: C57BL/6N MGI:6158642


Genotype
MGI:6158642
hm1
Allelic
Composition
Slc25a32Gt(IST11480G10)Tigm/Slc25a32Gt(IST11480G10)Tigm
Genetic
Background
involves: C57BL/6N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc25a32Gt(IST11480G10)Tigm mutation (0 available); any Slc25a32 mutation (20 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• initial heterozygote crosses failed to produce any viable homozygous mutant pups
• in utero calcium formate supplementation resulted in 78.1% of embryos completing NTC; however, no fetuses survived beyond E15.5

nervous system
• maternal 5-methyltetrahydrofolate (5-mTHF) supplementation failed to prevent NTDs with no identifiable embryos completing NTC until E12.5
• at E10.5-E12.5, all embryos (60 out of 60 analyzed) failed to complete neural tube closure (NTC); 18 out of 60 embryos showed NTC defects from the forebrain to the midbrain, 7 embryos had open midbrain NTDs, 26 embryos showed an open neural tube extending from the midbrain to the hindbrain, and 9 embryos exhibited craniorachischisis
• maternal calcium formate supplementation of heterozygous dams partially rescued NTDs, with 78.1% of embryos completing NTC at E10-E15.5, while the remaining 21.9% fetuses displayed open NTDs
• all embryos showed open rostral NTDs at either E10.5 or E12.5
• H&E staining revealed that E11.5 embryos had unfused neural folds at the level of the forebrain and the midbrain, unlike wild-type and heterozygous controls
• at E10.5, a subset of embryos showed open neural tubes extending the length from the forebrain to the hindbrain, as well as craniofacial defects
• at E10.5, some embryos exhibited craniorachischisis, with an open neural tube extending from the forebrain to the cervical boundary
• at E10.5-E12.5, nine out of 60 embryos with NTDs showed craniorachischisis
• at E10.5, most embryos with neural tube defects (NTDs) exhibited exencephaly
• at E12.5, NTD-affected embryos primarily showed exencephaly

embryo
• at E10.5-E12.5, all embryos (60 out of 60 analyzed) failed to complete neural tube closure (NTC); 18 out of 60 embryos showed NTC defects from the forebrain to the midbrain, 7 embryos had open midbrain NTDs, 26 embryos showed an open neural tube extending from the midbrain to the hindbrain, and 9 embryos exhibited craniorachischisis
• maternal calcium formate supplementation of heterozygous dams partially rescued NTDs, with 78.1% of embryos completing NTC at E10-E15.5, while the remaining 21.9% fetuses displayed open NTDs
• maternal 5-methyltetrahydrofolate (5-mTHF) supplementation failed to prevent NTDs with no identifiable embryos completing NTC until E12.5
• all embryos showed open rostral NTDs at either E10.5 or E12.5
• H&E staining revealed that E11.5 embryos had unfused neural folds at the level of the forebrain and the midbrain, unlike wild-type and heterozygous controls
• at E10.5, a subset of embryos showed open neural tubes extending the length from the forebrain to the hindbrain, as well as craniofacial defects
• at E10.5, some embryos exhibited craniorachischisis, with an open neural tube extending from the forebrain to the cervical boundary
• at E10.5-E12.5, nine out of 60 embryos with NTDs showed craniorachischisis

craniofacial
• at E10.5, a subset of embryos showed open neural tubes extending the length from the forebrain to the hindbrain, as well as craniofacial defects





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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory