Analysis Tools
mortality/aging
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• mice surviving to weaning exhibit a high rate of mortality, with median survival of 48 weeks compared to 82 weeks for wild-type mice
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• newborns frequently die during the perinatal period
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growth/size/body
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• mice surviving to weaning are smaller
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• body weight is decreased in mice surviving to weaning
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skeleton
cellular
aneuploidy
(
J:325276
)
|
• MEFs exhibit genetic instability, with 56% of metaphases being neither diploid nor tetraploid, with chromosome counts ranging from 29 to 79
• however, no intrachromosomal rearrangements are detected in metaphase chromosomes, MEFs are not sensitized to DNA damage induced by either ionizing radiation or topotecan, mitotic spindle checkpoint is intact in MEFs and MEFs show no evidence of multipolar spindles or abnormal microtubule architecture
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• MEFS exhibit a defect in mitotic chromosome segregation
• however, no increase in DNA double strand breaks is seen indicating no defect in DNA repair
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• MEFs exhibit alternations in pericentric heterochromatin
• MEFS exhibit a high level of resistance to the anti-proliferative effects of the histone deacetylase inhibitor trichostatin A
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• early passage mouse embryonic fibroblasts (MEFs) exhibit a slower growth rate and reach plateau earlier than wild-type MEFs, with doubling time for cells of 1.6 days vs. 1.1 days for wild-type MEFs
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neoplasm
| N |
• a small hepatoma was seen in a mouse that died at 48 weeks of age but no malignancies were seen in 3 other mice that survived beyond 24 weeks of age
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reproductive system
| N |
• both males and females are fertile
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