Phenotypes associated with this allele

• Allele Symbol: Dgcr8^Gt(XG058)Byg
• Allele Name: gene trap XG058, BayGenomics
• Allele ID: MGI:4125742
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dgcr8^Gt(XG058)Byg/Dgcr8^Gt(XG058)Byg	involves: 129P2/OlaHsd * C57BL/6	MGI:5522555
ht2	Dgcr8^Gt(XG058)Byg/Dgcr8^+	B6.129P2-Dgcr8^Gt(XG058)Byg	MGI:5559172
ht3	Dgcr8^Gt(XG058)Byg/Dgcr8^+	involves: 129P2/OlaHsd * C57BL/6	MGI:5522556

Genotype
MGI:5522555

hm1

• Allelic Composition: Dgcr8^Gt(XG058)Byg/Dgcr8^Gt(XG058)Byg
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:198659 )

• mice die around E5 due to a generalized cell proliferation defects

Genotype
MGI:5559172

ht2

• Allelic Composition: Dgcr8^Gt(XG058)Byg/Dgcr8⁺
• Genetic Background: B6.129P2-Dgcr8^Gt(XG058)Byg

nervous system

abnormal neuronal migration ( J:201966 )

• decrease in the chemotactic responsiveness of medial ganglionic eminence derived cells to CXCL12

• migration defect in dentate precursor cells in the hippocampus

abnormal brain interneuron morphology ( J:201966 )

• distribution is altered at E18.5

decreased dentate gyrus size ( J:201966 )

• decrease in volume at P0

cellular

abnormal neuronal migration ( J:201966 )

• decrease in the chemotactic responsiveness of medial ganglionic eminence derived cells to CXCL12

• migration defect in dentate precursor cells in the hippocampus

Genotype
MGI:5522556

ht3

• Allelic Composition: Dgcr8^Gt(XG058)Byg/Dgcr8⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

behavior/neurological

behavior/neurological phenotype ( J:198659 )

N • mice exhibit normal locomotor activity in an open field test

impaired spatial learning ( J:198659 )

♂ • male mice exhibit impaired performance in the Morris Water maze compared with wild-type mice

abnormal spatial working memory ( J:198659 )

♂ • male, but not female, mice exhibit reduced spontaneous alternation in a Y-maze compared with wild-type mice

♂ • however, number of arm entries is normal and treatment with IGF2 rescues phenotype

behavioral despair ( J:198659 )

♂ • male mice exhibit increased immobility in a forced swim test compared with wild-type mice

abnormal nest building behavior ( J:198659 )

• impaired

nervous system

nervous system phenotype ( J:198659 )

N • mice exhibit normal morphological development of the brain

N • mice exhibit normal apoptosis in the lateral ventricle and hippocampal dentate gyrus

abnormal neuronal precursor proliferation ( J:198659 )

• adult neural progenitor cells exhibit decreased proliferation in the hippocampal dentate gyrus compared to in wild-type mice

• IGF2-stimulated neural progenitor cell proliferation is increased in vitro and in vivo compared with wild-type cell

• however, proliferation in the subventricular zone is normal

cellular

abnormal neuronal precursor proliferation ( J:198659 )

• adult neural progenitor cells exhibit decreased proliferation in the hippocampal dentate gyrus compared to in wild-type mice

• IGF2-stimulated neural progenitor cell proliferation is increased in vitro and in vivo compared with wild-type cell

• however, proliferation in the subventricular zone is normal