Phenotypes associated with this allele

• Allele Symbol: Sh3glb2^{Gt(OST224737)Lex}
• Allele Name: gene trap OST224737, Lexicon Genetics
• Allele ID: MGI:4240757
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sh3glb2^Gt(OST224737)Lex/Sh3glb2^Gt(OST224737)Lex	B6.129S5-Sh3glb2^Gt(OST224737)Lex	MGI:7489685

Genotype
MGI:7489685

hm1

• Allelic Composition: Sh3glb2^{Gt(OST224737)Lex}/Sh3glb2^{Gt(OST224737)Lex}
• Genetic Background: B6.129S5-Sh3glb2^{Gt(OST224737)Lex}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

cellular phenotype ( J:246421 )

N • MEFs transduced with lentivirus encoding the BH3-only protein BCL2 modifying factor (BMF) show no significant differences in cell viability relative to BMF-treated wild-type MEFs

N • following treatment with potent apoptosis inducers actinomycin D or staurosporine, MEFs show no significant differences in cell death relative to similarly treated wild-type MEFs

impaired autophagy ( J:246421 )

• MEFs exhibit a significant reduction in starvation-induced autophagic flux

abnormal endocytosis ( J:246421 )

• in vitro, MEFs exhibit impaired endosomal maturation, as shown by suppressed endosomal acidification during fluid-phase endocytosis, and a significant decrease in starvation-induced autophagic flux

• consistent with impaired endosome acidification, MEFs infected with mouse-adapted influenza A virus H1N1 strain A/PR 8/34 (PR8) (a member of the Orthomyxoviridiae family of enveloped viruses that is internalized through receptor-mediated endocytosis and trafficked to late endosomes) show a significant delay in both the nuclear and cytoplasmic accumulation of viral RNA-binding nucleoprotein relative to wild-type MEFs, suggesting a delay in viral RNA nuclear entry and replication

• however, internalization of influenza A virus into host cells is normal

homeostasis/metabolism

impaired autophagy ( J:246421 )

• MEFs exhibit a significant reduction in starvation-induced autophagic flux

abnormal pH regulation ( J:246421 )

• following incubation with pHrodo-Red dextran, MEFs show a normal time-dependent increase in fluorescence intensity that reaches a plateau ~5 min after addition of the dye; however, maximal fluorescence intensity of pHrodo-Red dextran is significantly lower than that in wild-type MEFs, suggesting impaired endosome acidification

• in contrast, MEFs exhibit normal uptake of FITC-dextran, indicating normal plasma membrane internalization during fluid-phase endocytosis

immune system

abnormal response to infection ( J:246421 )

• in vitro, MEFs infected with mouse-adapted influenza A virus H1N1 strain A/PR 8/34 (PR8) show delayed nuclear translocation and replication of the viral RNA genome

mortality/aging

mortality/aging ( J:246421 )

N • pups are born with normal ratios of Mendelian inheritance

embryo

embryo phenotype ( J:246421 )

N • pups are indistinguishable from wild-type controls, indicating normal embryonic development