Phenotypes associated with this allele

• Allele Symbol: Atg4d^{Gt(OST254045)Lex}
• Allele Name: gene trap OST254045, Lexicon Genetics
• Allele ID: MGI:4252827
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Atg4d^Gt(OST254045)Lex/Atg4d^Gt(OST254045)Lex Tg(CAG-EGFP/Map1lc3b)53Nmz/0	involves: 129S5/SvEvBrd * C57BL/6J * C57BL/6NCrlj * DBA/2J	MGI:6827352

Genotype
MGI:6827352

cx1

• Allelic Composition: Atg4d^{Gt(OST254045)Lex}/Atg4d^{Gt(OST254045)Lex}; Tg(CAG-EGFP/Map1lc3b)53Nmz/0
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J * C57BL/6NCrlj * DBA/2J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal Purkinje cell morphology ( J:313858 )

• misaligned and reduced size cell body with abnormally shaped nucleus and high numbers of autophagosomes

abnormal cerebellar molecular layer ( J:313858 )

• abnormal at 2 months persisting at 5 months

thin cerebellar molecular layer ( J:313858 )

• with reactive gliosis and synaptic clusters of GABA-A receptors

gliosis ( J:313858 )

• in the cerebellar molecular layer

neurodegeneration ( J:313858 )

• age-dependent cerebellar neurodegeneration

Purkinje cell degeneration ( J:313858 )

• in aged mice without increased apoptosis

behavior/neurological

impaired long-term object recognition memory ( J:313858 )

• impaired

increased thigmotaxis ( J:313858 )

limb grasping ( J:313858 )

• at 2 and 5 months, more pronounced with age

ataxia ( J:313858 )

• cerebellar ataxia

impaired coordination ( J:313858 )

• at 2 and 5 months of age on a rotarod and balance beam, more pronounced with age

decreased grip strength ( J:313858 )

• at 2 and 5 months, more pronounced with age

long stride length ( J:313858 )

• at 2 and 5 months, more pronounced with age

homeostasis/metabolism

abnormal autophagosome formation ( J:313858 )

• increased and smaller size GFP-LC3B+ structures in mouse embryonic fibroblasts

• increased ATG8-like proteins in GFP-LCB3 puncta in the liver, heart, and skeletal muscle of mice fed ad libitum or after 24 h fast

• however, autophagy flux is normal

cellular

abnormal autophagosome formation ( J:313858 )

• increased and smaller size GFP-LC3B+ structures in mouse embryonic fibroblasts

• increased ATG8-like proteins in GFP-LCB3 puncta in the liver, heart, and skeletal muscle of mice fed ad libitum or after 24 h fast

• however, autophagy flux is normal