All Phenotypes Vrk1<Gt(RRR178)Byg> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}	B6.129P2-Vrk1^{Gt(RRR178)Byg}	MGI:5910363
hm2	Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}	FVB.129P2(B6)-Vrk1^{Gt(RRR178)Byg}	MGI:6865660
hm3	Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}	involves: 129P2/OlaHsd * C57BL/6	MGI:4421834
cx4	Trp53^tm1Tyj/Trp53^tm1Tyj Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6	MGI:6865653

Allelic Composition	Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}
Genetic Background	B6.129P2-Vrk1^{Gt(RRR178)Byg}

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Vrk1^{Gt(RRR178)Byg} mutation (0 available); any Vrk1 mutation (42 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

early reproductive senescence ( J:171525 )

• each of the two males tested produced one litter at 42 days postpartum (dpp); however, both males failed to produce a second litter by 98 dpp

reproductive system

abnormal oocyte morphology ( J:207272 )

• 3-D transparent images of the antral follicle and the Graffian follicle showed that the sizes of oocytes are smaller than those in wild-type follicles

• at 6 weeks of age, oocyte volumes in antral and Graffian follicles are decreased by 42% and 37%, respectively, indicating defects in oocyte quality at the preovulatory stage

decreased secondary ovarian follicle number ( J:207272 )

• average number of pre-antral follicles (equal to or larger than 50um in diameter) per ovary is reduced by 36%, 43% and 38%, respectively, in 6-, 16- and 43-week-old females

decreased tertiary ovarian follicle number ( J:207272 )

• average number of antral follicles per ovary is reduced by 47%, 22% and 46%, respectively, in 6-, 16- and 43-week-old females

• however, the volume of the antrum cavity in Graffian follicles is not altered at any age

impaired ovarian folliculogenesis ( J:207272 )

• expression levels of 17 genes involved in both early and later folliculogenesis are significantly decreased, suggesting defects in folliculogenesis

abnormal ovary tissue architecture ( J:207272 )

• at 6 weeks of age, 3-D segmented images of the whole ovary showed that the volume fraction of the stroma is increased from 56% to 64% relative to the wild-type ovary

early reproductive senescence ( J:171525 )

• each of the two males tested produced one litter at 42 days postpartum (dpp); however, both males failed to produce a second litter by 98 dpp

female infertility ( J:171525 )

• six-week-old females fail to produce litters despite the presence of copulatory plugs

male infertility ( J:171525 )

• males fail to produce a second litter after 42 dpp

cellular

abnormal oocyte morphology ( J:207272 )

• 3-D transparent images of the antral follicle and the Graffian follicle showed that the sizes of oocytes are smaller than those in wild-type follicles

• at 6 weeks of age, oocyte volumes in antral and Graffian follicles are decreased by 42% and 37%, respectively, indicating defects in oocyte quality at the preovulatory stage

endocrine/exocrine glands

decreased secondary ovarian follicle number ( J:207272 )

• average number of pre-antral follicles (equal to or larger than 50um in diameter) per ovary is reduced by 36%, 43% and 38%, respectively, in 6-, 16- and 43-week-old females

decreased tertiary ovarian follicle number ( J:207272 )

• average number of antral follicles per ovary is reduced by 47%, 22% and 46%, respectively, in 6-, 16- and 43-week-old females

• however, the volume of the antrum cavity in Graffian follicles is not altered at any age

impaired ovarian folliculogenesis ( J:207272 )

• expression levels of 17 genes involved in both early and later folliculogenesis are significantly decreased, suggesting defects in folliculogenesis

abnormal ovary tissue architecture ( J:207272 )

• at 6 weeks of age, 3-D segmented images of the whole ovary showed that the volume fraction of the stroma is increased from 56% to 64% relative to the wild-type ovary

Allelic Composition	Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}
Genetic Background	FVB.129P2(B6)-Vrk1^{Gt(RRR178)Byg}

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Vrk1^{Gt(RRR178)Byg} mutation (0 available); any Vrk1 mutation (42 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

female infertility ( J:171525 )

• six-week-old females fail to produce litters despite the presence of copulatory plugs

male infertility ( J:171525 )

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

decreased male germ cell number ( J:156653 , J:171525 )

• progressive loss of meitotic cells and spermatids with age (J:156653)

• however, the earliest spermatogonia are retained (J:156653)

• significant loss of male germ cells at all stages of meiosis and differentiation by 14 weeks of age (J:171525)

abnormal oogenesis ( J:171525 )

• females exhibit multiple defects during oocyte development, including delays in meiotic progression and lagging chromosomes during metaphase II

• however, cultured germinal vesicle (GV) oocytes obtained from antral follicles show no gross defects in histone H2A Thr120 and histone H3 Ser10 phosphorylation

abnormal female meiosis ( J:171525 )

• in vitro, many oocytes obtained from the ampulla of superovulated females fail to reach MII arrest

• only 15.6% of GV stage oocytes reach the "parachute" stage versus 49.1% in wild-type females; however, normal numbers of oocytes are found at later stages (prometaphase and metaphase I)

• only 22.5% of GV stage oocytes ultimately reach metaphase II versus 54.5% in wild-type females

• 42.9% of those GV stage oocytes that reach metaphase II exhibit lagging chromosomes versus 2.7% in wild-type females; as a result, less than 13% of oocytes reach metaphase II properly

• however, spindle formation and spindle length remain normal and the overall chromosome number is unaffected

impaired polar body extrusion ( J:171525 )

• in vitro, only 27.9% of GV stage oocytes obtained from antral follicles of superovulated females show polar body extrusion versus 59.9% in wild-type females; 67.7% progress through germinal vesicle breakdown (GVBD) but fail to extrude a polar body versus only 39.2% in wild-type females, indicating meiotic delays

abnormal seminiferous tubule morphology ( J:156653 )

• by 11 weeks of age all tubules contain only Sertoli cells

• as early as 6 weeks of age, a striking loss of cellular density is seen in many tubules with only Sertoli cells present

seminiferous tubule degeneration ( J:156653 , J:171525 )

(J:156653)

• marked loss/degeneration of the seminiferous tubular epithelium by 14 weeks of age (J:171525)

small testis ( J:156653 )

• decrease in the size of the testis in post pubescent mice, although the overall body sizes of all mice are comparable

decreased testis weight ( J:171525 )

• testis weight is significantly decreased at all ages examined between 6-8 and >12 weeks of age

early reproductive senescence ( J:171525 )

• all males are fertile at the onset of sexual maturity but become sterile by 10 weeks of age

abnormal spermatogenesis ( J:156653 , J:171525 )

• largely devoid of GCNA+ cells in tubules, a marker of spermatogonial stem cells, mitotic spermatogonia, and cells in the prediplotene stages of meiosis I (J:156653)

• PCNA + cells, a marker of proliferating type A and B spermatogonia, are present at 4 weeks but by 14 weeks, 90% of tubules devoid (J:156653)

• adult mice are devoid of KIT+, an early spermatogonial differentiation marker, cells (J:156653)

• progressive reduction of all stages of spermatogenesis within testes, starting at 10 weeks of age (J:171525)

• no meiotic arrest or defects in synaptonemal complex formation are observed (J:171525)

azoospermia ( J:156653 )

abnormal spermatid morphology ( J:156653 )

• progressive loss of spermatids with age

abnormal spermatogonia proliferation ( J:156653 )

• progressive loss of proliferating spermatogonia

abnormal epididymis morphology ( J:156653 )

• at 6 weeks of age only 20% of lumens have sperm

• at 12 weeks of age, the lumens of the epididymal tubules are devoid of sperm

female infertility ( J:156653 , J:171525 )

(J:156653)

• females fail to produce litters or show any signs of pregnancy (J:171525)

• however, deposition of seminal fluid is always observed, and ovaries contain all stages of folliculogenesis (including corpora lutea) indicating successful ovulation (J:171525)

male infertility ( J:156653 , J:171525 )

• in fertility tests, only 1 of 9 males produce a single litter, all of the other males fail to produce any litters (J:156653)

• all males are sterile by 10 weeks of age; males never sire a litter after 69 days postpartum (dpp), despite continued presence of copulatory plugs (J:171525)

impaired fertilization ( J:171525 )

• in vitro, most oocytes obtained from the ampulla of superovulated females show absence of maternal and paternal pronuclei and no embryonic development, indicating failure of fertilization

• some oocytes progress to the 2-cell stage but not beyond, and all oocytes degenerate after 96 hours in culture

cellular

abnormal spermatid morphology ( J:156653 )

• progressive loss of spermatids with age

decreased male germ cell number ( J:156653 , J:171525 )

• progressive loss of meitotic cells and spermatids with age (J:156653)

• however, the earliest spermatogonia are retained (J:156653)

• significant loss of male germ cells at all stages of meiosis and differentiation by 14 weeks of age (J:171525)

azoospermia ( J:156653 )

abnormal oogenesis ( J:171525 )

• females exhibit multiple defects during oocyte development, including delays in meiotic progression and lagging chromosomes during metaphase II

• however, cultured germinal vesicle (GV) oocytes obtained from antral follicles show no gross defects in histone H2A Thr120 and histone H3 Ser10 phosphorylation

abnormal female meiosis ( J:171525 )

• in vitro, many oocytes obtained from the ampulla of superovulated females fail to reach MII arrest

• only 15.6% of GV stage oocytes reach the "parachute" stage versus 49.1% in wild-type females; however, normal numbers of oocytes are found at later stages (prometaphase and metaphase I)

• only 22.5% of GV stage oocytes ultimately reach metaphase II versus 54.5% in wild-type females

• 42.9% of those GV stage oocytes that reach metaphase II exhibit lagging chromosomes versus 2.7% in wild-type females; as a result, less than 13% of oocytes reach metaphase II properly

• however, spindle formation and spindle length remain normal and the overall chromosome number is unaffected

impaired polar body extrusion ( J:171525 )

abnormal spermatogonia proliferation ( J:156653 )

• progressive loss of proliferating spermatogonia

mortality/aging

early reproductive senescence ( J:171525 )

• all males are fertile at the onset of sexual maturity but become sterile by 10 weeks of age

endocrine/exocrine glands

abnormal seminiferous tubule morphology ( J:156653 )

• as early as 6 weeks of age, a striking loss of cellular density is seen in many tubules with only Sertoli cells present

• by 11 weeks of age all tubules contain only Sertoli cells

seminiferous tubule degeneration ( J:156653 , J:171525 )

(J:156653)

• marked loss/degeneration of the seminiferous tubular epithelium by 14 weeks of age (J:171525)

small testis ( J:156653 )

• decrease in the size of the testis in post pubescent mice, although the overall body sizes of all mice are comparable

decreased testis weight ( J:171525 )

• testis weight is significantly decreased at all ages examined between 6-8 and >12 weeks of age

Allelic Composition	Trp53^tm1Tyj/Trp53^tm1Tyj Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}
Genetic Background	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Trp53^tm1Tyj mutation (12 available); any Trp53 mutation (240 available)
Vrk1^{Gt(RRR178)Byg} mutation (0 available); any Vrk1 mutation (42 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

decreased tumor-free survival time ( J:171525 )

• mice begin to succumb to tumors around 3 months of age, as seen in single Trp53^tm1Tyj homozygotes

early reproductive senescence ( J:171525 )

• all three males tested produced one litter between 35 and 50 days postpartum (dpp), and two sired litters after 50 dpp (one at 68 dpp and one at 69 dpp); however, males become sterile after 69 dpp, similar to single Vrk1^{Gt(RRR178)Byg} homozygotes

neoplasm

increased tumor incidence ( J:171525 )

reproductive system

seminiferous tubule degeneration ( J:171525 )

• marked loss/degeneration of the seminiferous tubular epithelium by 12 weeks of age

decreased testis weight ( J:171525 )

• testis weight is significantly decreased at all ages examined between 6-8 and >12 weeks of age, similar to single Vrk1^{Gt(RRR178)Byg} homozygotes

early reproductive senescence ( J:171525 )

abnormal spermatogenesis ( J:171525 )

• progressive decline in spermatogenesis, starting at 8 weeks of age

• loss of spermatogenesis occurs earlier than in single Vrk1^{Gt(RRR178)Byg} homozygotes

female infertility ( J:171525 )

• females fail to produce litters or show any signs of pregnancy, similar to single Vrk1^{Gt(RRR178)Byg} female homozygotes

• however, copulatory plugs are observed

male infertility ( J:171525 )

• males fail to sire a litter after 69 days postpartum (dpp), despite continued presence of copulatory plugs

endocrine/exocrine glands

seminiferous tubule degeneration ( J:171525 )

• marked loss/degeneration of the seminiferous tubular epithelium by 12 weeks of age

decreased testis weight ( J:171525 )

• testis weight is significantly decreased at all ages examined between 6-8 and >12 weeks of age, similar to single Vrk1^{Gt(RRR178)Byg} homozygotes

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 12/10/2024 MGI 6.24