Analysis Tools
mortality/aging
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• homozygotes are viable throughout embryogenesis and at E18.5; however, no homozygotes are recovered by P11
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growth/size/body
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• at E18.5, homozygotes are markedly smaller than wild-type controls
• body size is significantly reduced at E13.5 and E14.5
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• at E13.5 and E14.5, homozygotes are developmentally delayed by 1 day relative to wild-type controls, as noted by eye pigmentation and limb development
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limbs/digits/tail
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• at E14.5, limb development is delayed relative to that in wild-type controls
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nervous system
| N |
• at E18.5, brain size is proportionally reduced relative to body length, suggesting that brain development is largely normal
• although cortical thickness, number of TBR2+ intermediate neural progenitors, and thickness of the TUJ1+ neuronal layer are reduced at E14.5, these differences are largely due to developmental delay, as E14.5 values are similar to those in E13.5 wild-type brains
• no significant apoptosis is noted in the cortex at E14.5
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