Phenotypes associated with this allele

• Allele Symbol: Daam1^{Gt(RRT390)Byg}
• Allele Name: gene trap RRT390, BayGenomics
• Allele ID: MGI:4338561
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Daam1^Gt(RRT390)Byg/Daam1^Gt(RRT390)Byg	involves: 129P2/OlaHsd * C57BL/6J	MGI:4880767
cn2	Daam1^Gt(RRT390)Byg/Daam1^Gt(RRT390)Byg Nkx2-5^tm1(cre)Rjs/Nkx2-5^+	involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6J	MGI:4880768

Genotype
MGI:4880767

hm1

• Allelic Composition: Daam1^{Gt(RRT390)Byg}/Daam1^{Gt(RRT390)Byg}
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased survivor rate ( J:167714 )

lethality throughout fetal growth and development, incomplete penetrance ( J:167714 )

• partial lethality between E14.5 and E16.5

neonatal lethality, incomplete penetrance ( J:167714 )

• partial

homeostasis/metabolism

pericardial effusion ( J:167714 )

• present in most mice

cyanosis ( J:167714 )

• in mice that die neonatally

impaired wound healing ( J:167714 )

• in MEFs

cardiovascular system

cardiovascular system phenotype ( J:167714 )

N • despite showing some cardiac abnormalities, no defects in cardiac systolic function are detected in survivors at 2 or 6 months of age

abnormal fetal cardiomyocyte morphology ( J:167714 )

• at E12.5 in vivo less F-actin is present in the ventricular myocardia and myocardia around the outflow tract

fetal cardiomyocyte disarray ( J:167714 )

• in cultured E12.5 cardiomyocytes randomly orientated and aggregated striated F-actin is seen

double outlet right ventricle ( J:167714 )

abnormal heart ventricle morphology ( J:167714 )

• ventricular noncompaction in embryos

• all survivors display varying degrees of ventricular noncompaction

ventricular septal defect ( J:167714 )

abnormal heart left ventricle morphology ( J:167714 )

• surviving mice show an increase in diastolic left ventricular wall thickness

pericardial effusion ( J:167714 )

• present in most mice

muscle

abnormal sarcomere morphology ( J:167714 )

• exhibit weaker and more diffuse staining patterns for both alpha actin and alpha actinin

• exhibit fewer striations and randomized or chaotic sarcomeric organization

• sparsely distributed and significantly shorter in width

abnormal muscle physiology ( J:167714 )

• defects in cardiomyocyte cell-cell adhesion with adherens junctions that display prominent plaques and misalignment alongside the plasma membrane

• however, desmosomes appear normal

cellular

abnormal cell adhesion ( J:167714 )

• impaired in MEFs

abnormal fibroblast physiology ( J:167714 )

• lamellipodia formation in MEFs at 2 hours after serum restoration is reduced

growth/size/body

decreased fetal size ( J:167714 )

• at E14.5

Genotype
MGI:4880768

cn2

• Allelic Composition: Daam1^{Gt(RRT390)Byg}/Daam1^{Gt(RRT390)Byg}; Nkx2-5^tm1(cre)Rjs/Nkx2-5⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6J

mortality/aging

mortality/aging ( J:167714 )

N • cre mediated reversion rescues the lethality seen in homozygous mutants lacking cre expression in the heart

cardiovascular system

cardiovascular system phenotype ( J:167714 )

N • cre mediated reversion rescues cardiac defects seen in homozygous mutants lacking cre expression in the heart

embryo

decreased embryo size ( J:167714 )

• most embryos are smaller than wild-type littermates

growth/size/body

decreased embryo size ( J:167714 )

• most embryos are smaller than wild-type littermates

decreased body size ( J:167714 )

• most pups are smaller than wild-type littermates