All Phenotypes Themis<tm1Gasc> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Themis^tm1Gasc/Themis^tm1Gasc	B6.129S-Themis^tm1Gasc	MGI:4353111
cx2	Tg(TcraTcrb)1100Mjb/? Themis^tm1Gasc/Themis^tm1Gasc	involves: 129S6/SvEvTac * C57BL/6	MGI:4353114
cx3	H2^b/H2^d Themis^tm1Gasc/Themis^tm1Gasc	involves: 129S6/SvEvTac * C57BL/6 * C57BL/10 * DBA/2	MGI:4353112
cx4	Tg(TcraH-Y,TcrbH-Y)71Vbo/? Themis^tm1Gasc/Themis^tm1Gasc	involves: 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:4353113
cx5	Tg(TcrAND)53Hed/? Themis^tm1Gasc/Themis^tm1Gasc	involves: 129S6/SvEvTac * C57BL/6 * SJL	MGI:4353115

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased T cell proliferation ( J:151074 )

• T cell proliferation in response to anti-CD3 stimulation is very poor compared to controls

abnormal thymus medulla morphology ( J:151074 )

• thymus medulla is smaller and more fragmented than controls

decreased thymocyte number ( J:151074 )

• the number of thymocytes with an intermediate expression of CD69 and TCR is decreased in these mice

• there is considerable depletion of TCR^hiCD69^hi thymocytes

• these TCR^hiCD69^hi thymocytes are double-positive or CD8-single positive compared to wild-type controls where this population is almost exclusively single-positive

abnormal positive T cell selection ( J:151074 )

• TCR and CD69 expression levels suggest positive selection is mostly blocked at its earliest stage during which CD8 downregulation is initiated

• bone marrow chimera experiments demonstrate that positive selection is intrinsic to the thymocytes

increased double-positive T cell number ( J:151074 )

• the proportion and number of double-positive thymocytes is slightly but significantly greater

decreased CD4-positive, alpha-beta T cell number ( J:151074 )

• CD4⁺ single-positive thymocyte numbers are reduced by about half

• CD4⁺ T cells in the periphery are reduced about 10-fold

decreased CD8-positive, alpha-beta T cell number ( J:151074 )

• CD8⁺ single-positive thymocyte numbers are reduced by about half

• CD8⁺ T cells in the periphery are reduced about 5-fold

abnormal memory T cell morphology ( J:151074 )

• the relative frequency of memory T cells in the periphery is increased, probably indicating an expansion of a small population of nave T cells

abnormal regulatory T cell morphology ( J:151074 )

• FoxP3⁺ regulatory T cells are increased in frequency (21%) but only have half the absolute numbers as wild-type controls

hematopoietic system

decreased T cell proliferation ( J:151074 )

• T cell proliferation in response to anti-CD3 stimulation is very poor compared to controls

abnormal thymus medulla morphology ( J:151074 )

• thymus medulla is smaller and more fragmented than controls

decreased thymocyte number ( J:151074 )

• the number of thymocytes with an intermediate expression of CD69 and TCR is decreased in these mice

• there is considerable depletion of TCR^hiCD69^hi thymocytes

• these TCR^hiCD69^hi thymocytes are double-positive or CD8-single positive compared to wild-type controls where this population is almost exclusively single-positive

abnormal positive T cell selection ( J:151074 )

• TCR and CD69 expression levels suggest positive selection is mostly blocked at its earliest stage during which CD8 downregulation is initiated

• bone marrow chimera experiments demonstrate that positive selection is intrinsic to the thymocytes

increased double-positive T cell number ( J:151074 )

• the proportion and number of double-positive thymocytes is slightly but significantly greater

decreased CD4-positive, alpha-beta T cell number ( J:151074 )

• CD4⁺ single-positive thymocyte numbers are reduced by about half

• CD4⁺ T cells in the periphery are reduced about 10-fold

decreased CD8-positive, alpha-beta T cell number ( J:151074 )

• CD8⁺ single-positive thymocyte numbers are reduced by about half

• CD8⁺ T cells in the periphery are reduced about 5-fold

abnormal memory T cell morphology ( J:151074 )

• the relative frequency of memory T cells in the periphery is increased, probably indicating an expansion of a small population of nave T cells

abnormal regulatory T cell morphology ( J:151074 )

• FoxP3⁺ regulatory T cells are increased in frequency (21%) but only have half the absolute numbers as wild-type controls

endocrine/exocrine glands

abnormal thymus medulla morphology ( J:151074 )

• thymus medulla is smaller and more fragmented than controls

decreased thymocyte number ( J:151074 )

• there is considerable depletion of TCR^hiCD69^hi thymocytes

• the number of thymocytes with an intermediate expression of CD69 and TCR is decreased in these mice

• these TCR^hiCD69^hi thymocytes are double-positive or CD8-single positive compared to wild-type controls where this population is almost exclusively single-positive

cellular

decreased T cell proliferation ( J:151074 )

• T cell proliferation in response to anti-CD3 stimulation is very poor compared to controls

Allelic Composition	Tg(TcraTcrb)1100Mjb/? Themis^tm1Gasc/Themis^tm1Gasc
Genetic Background	involves: 129S6/SvEvTac * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcraTcrb)1100Mjb mutation (6 available)
Themis^tm1Gasc mutation (1 available); any Themis mutation (57 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal positive T cell selection ( J:151074 )

• thymocytes fail to exit the CD4^hiCD8^intstage to the CD8 single-positive stage

increased double-positive T cell number ( J:151074 )

• the proportion and number of double-positive thymocytes is greater than controls

decreased CD8-positive, alpha-beta T cell number ( J:151074 )

• very few single-positive thymocytes are generated in the thymus

hematopoietic system

abnormal positive T cell selection ( J:151074 )

• thymocytes fail to exit the CD4^hiCD8^intstage to the CD8 single-positive stage

increased double-positive T cell number ( J:151074 )

• the proportion and number of double-positive thymocytes is greater than controls

decreased CD8-positive, alpha-beta T cell number ( J:151074 )

• very few single-positive thymocytes are generated in the thymus

Allelic Composition	H2^b/H2^d Themis^tm1Gasc/Themis^tm1Gasc
Genetic Background	involves: 129S6/SvEvTac * C57BL/6 * C57BL/10 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2^b mutation (30 available); any H2 mutation (282 available)
H2^d mutation (23 available); any H2 mutation (282 available)
Themis^tm1Gasc mutation (1 available); any Themis mutation (57 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal negative T cell selection ( J:151074 )

• superantigen-mediated deletion of T cells expressing Vbeta5, Vbeta11, and Vbeta12 is less efficient in these mice

immune system

abnormal negative T cell selection ( J:151074 )

• superantigen-mediated deletion of T cells expressing Vbeta5, Vbeta11, and Vbeta12 is less efficient in these mice

Allelic Composition	Tg(TcraH-Y,TcrbH-Y)71Vbo/? Themis^tm1Gasc/Themis^tm1Gasc
Genetic Background	involves: 129S6/SvEvTac * C57BL/6 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcraH-Y,TcrbH-Y)71Vbo mutation (4 available)
Themis^tm1Gasc mutation (1 available); any Themis mutation (57 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal negative T cell selection ( J:151074 )

• the negative selection that normally occurs to transgenic thymocytes in male mice is incomplete in these mice with some single-positive thymocytes present in the thymus

abnormal positive T cell selection ( J:151074 )

• the positive selection that normally occurs to transgeneic thymocytes in female mice is incomplete in these mice with few CD8 T cells being generated

hematopoietic system

abnormal negative T cell selection ( J:151074 )

• the negative selection that normally occurs to transgenic thymocytes in male mice is incomplete in these mice with some single-positive thymocytes present in the thymus

abnormal positive T cell selection ( J:151074 )

• the positive selection that normally occurs to transgeneic thymocytes in female mice is incomplete in these mice with few CD8 T cells being generated

Allelic Composition	Tg(TcrAND)53Hed/? Themis^tm1Gasc/Themis^tm1Gasc
Genetic Background	involves: 129S6/SvEvTac * C57BL/6 * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcrAND)53Hed mutation (2 available)
Themis^tm1Gasc mutation (1 available); any Themis mutation (57 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal positive T cell selection ( J:151074 )

• positive selection is almost completely blocked in these mice

increased double-positive T cell number ( J:151074 )

• the proportion and number of double-positive thymocytes is greater than controls

decreased CD4-positive, alpha-beta T cell number ( J:151074 )

• very few single-positive thymocytes are generated in the thymus

• peripheral CD4 T cell numbers are severely reduced

decreased CD8-positive, alpha-beta T cell number ( J:151074 )

• very few single-positive thymocytes are generated in the thymus

hematopoietic system

abnormal positive T cell selection ( J:151074 )

• positive selection is almost completely blocked in these mice

increased double-positive T cell number ( J:151074 )

• the proportion and number of double-positive thymocytes is greater than controls

decreased CD4-positive, alpha-beta T cell number ( J:151074 )

• very few single-positive thymocytes are generated in the thymus

• peripheral CD4 T cell numbers are severely reduced

decreased CD8-positive, alpha-beta T cell number ( J:151074 )

• very few single-positive thymocytes are generated in the thymus

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