Phenotypes associated with this allele

• Allele Symbol: Skap2^{Gt(VICTR20)21Lex}
• Allele Name: gene trap 21, Lexicon Genetics
• Allele ID: MGI:4353991
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Skap2^Gt(VICTR20)21Lex/Skap2^Gt(VICTR20)21Lex	B6.129S5-Skap2^Gt(VICTR20)21Lex	MGI:4353994

Genotype
MGI:4353994

hm1

• Allelic Composition: Skap2^{Gt(VICTR20)21Lex}/Skap2^{Gt(VICTR20)21Lex}
• Genetic Background: B6.129S5-Skap2^{Gt(VICTR20)21Lex}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal B cell activation ( J:101373 )

• B cell activation fails to increase binding of cells to fibronectin or ICAM-1

decreased B cell proliferation ( J:101373 )

• B cell proliferation in response to anti-IgM or LPS is significantly impaired compared to controls

• response to PMA-ionomycin or anti-CD40 + IL-4 was normal ruling out a general B cell defect

decreased IgG2a level ( J:101373 )

• IgG2a levels are about a third of that in controls

decreased IgM level ( J:101373 )

• IgM levels are less than half that of wild-type controls

abnormal dendritic cell physiology ( J:151350 )

• dendritic cells have enhanced migration to lymph nodes under inflammatory conditons

abnormal dendritic cell antigen presentation ( J:151350 )

• the establishment of antigen-specific stable conjugates between DCs and T cells is delayed significantly with mutant DCs

• this defect in immunological synapse formation results in defective T cell activation and proliferation when mutant DC present T cells with its cognate antigen

decreased susceptibility to experimental autoimmune encephalomyelitis ( J:101373 )

• mice develop the first clinical signs of MOG induced EAE 2-3 days earlier than controls

• the maximal mean clinical score of EAE is 1.2 compared to 2.2 in wild-type controls

• levels of MOG-specific IgM and IgG are decreased in mutant mice compared to controls

hematopoietic system

abnormal B cell activation ( J:101373 )

• B cell activation fails to increase binding of cells to fibronectin or ICAM-1

decreased B cell proliferation ( J:101373 )

• B cell proliferation in response to anti-IgM or LPS is significantly impaired compared to controls

• response to PMA-ionomycin or anti-CD40 + IL-4 was normal ruling out a general B cell defect

decreased IgG2a level ( J:101373 )

• IgG2a levels are about a third of that in controls

decreased IgM level ( J:101373 )

• IgM levels are less than half that of wild-type controls

behavior/neurological

abnormal behavioral response to addictive substance ( J:216839 )

• mutants exhibit sensitization after repeated amphetamine application as wild-type mice, however in reaction to amphetamine challenge, wild-type mice show an enhanced reaction compared to mutant mice

abnormal learning/memory/conditioning ( J:216839 )

• in the social memory test, mutants spend a similar amount of time with the novel unfamiliar mouse as with the former unfamiliar mouse, indicating that social memory is impaired

• however, sociability is similar to wild-type mice and spatial learning and memory in the Morris water maze are normal

increased anxiety-related response ( J:216839 )

• prior to application of conditioned stimulus, mutants show more freezing in reaction to the test environment

fast extinction of fear memory ( J:216839 )

• extinction of conditioned fear is accelerated, indicating a more rapid decline in fear memory

• however, fear conditioning is comparable to wild-type mice

cellular

decreased B cell proliferation ( J:101373 )

• B cell proliferation in response to anti-IgM or LPS is significantly impaired compared to controls

• response to PMA-ionomycin or anti-CD40 + IL-4 was normal ruling out a general B cell defect