Phenotypes associated with this allele

• Allele Symbol: Klrk1^tm1.1Bpol
• Allele Name: targeted mutation 1.1, Bojan Polic
• Allele ID: MGI:4355860
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Klrk1^tm1.1Bpol/Klrk1^tm1.1Bpol	C57BL/6-Klrk1^tm1.1Bpol	MGI:4355862

Genotype
MGI:4355862

hm1

• Allelic Composition: Klrk1^tm1.1Bpol/Klrk1^tm1.1Bpol
• Genetic Background: C57BL/6-Klrk1^tm1.1Bpol

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased susceptibility to Herpesvirales infection induced morbidity/mortality ( J:151878 )

• when infected with a LD50 dose of wild-type MCMV, about 90% of mice survive compared to 50-60% of wild-type

• when higher doses are given, mutant mice continue to have a higher survival rate than controls

immune system

abnormal NK cell differentiation ( J:151878 )

• there is a significant reduction of the NK cell population expressing c-Kit in the bone marrow as well as in the spleen

• the reduction of c-Kit⁺ NK cells occurs in the transition from immature to mature NK cells rather than earlier

• NK cells expressing Ly49A are reduced in the spleen and bone marrow

• in addition, NK populations in the bone marrow expressing CD11b^hi and CD43^hi are reduced as well as NK cells expressing Klrg1

• immature NK cells in the bone marrow have a higher proliferation rate and also have a faster differentiation and transition from the stage III to the more mature stage V

decreased B cell number ( J:151878 )

• T and B cell numbers are reduced in the spleen but not in other organs (i.e. lymph nodes, thymus, etc.)

decreased T cell number ( J:151878 )

• T and B cell numbers are reduced in the spleen but not in other organs (i.e. lymph nodes, thymus, etc.)

spleen hypoplasia ( J:151878 )

• spleen cellularity is reduced by a third to a half due to smaller numbers of T and B cells

• reduced lymphocytes numbers are restricted to the spleen as lymph node numbers are normal

abnormal NK cell physiology ( J:151878 )

• immature NK cells in the bone marrow have a higher proliferation rate

• NK cells are more susceptible to apoptosis in culture, and less responsive to the anti-apoptotic effects of IL-2 and IL-15

• NK cells produce more IFN-gamma than controls in response to Chinese Hamster Ovary cells, anti-NK1.1 stimulation, and to MCMV infection

impaired natural killer cell mediated cytotoxicity ( J:151878 )

• NK cells are less able to kill tumor cell lines expressing an NG2GD ligand

• NK cells are able to kill cells lacking MHC-I expression at the same efficiency as wild-type NK cells

increased interferon-gamma secretion ( J:151878 )

• NK cells have increased IFN-gamma secretion in response to Chinese Hamster Ovary cells, anti-NK1.1 stimulation, and to MCMV infection

decreased susceptibility to Herpesvirales infection ( J:151878 )

• mice are resistant to MCMV infection

• when infected with a strain of MCMV that won't trigger Ly49H-mediated cytolysis, mice have a 10-fold lower viral titer 4 days after infection

• NK cells in infected mice show faster maturation and/or migration in the spleen compared to controls

decreased susceptibility to Herpesvirales infection induced morbidity/mortality ( J:151878 )

• when infected with a LD50 dose of wild-type MCMV, about 90% of mice survive compared to 50-60% of wild-type

• when higher doses are given, mutant mice continue to have a higher survival rate than controls

hematopoietic system

abnormal NK cell differentiation ( J:151878 )

• there is a significant reduction of the NK cell population expressing c-Kit in the bone marrow as well as in the spleen

• the reduction of c-Kit⁺ NK cells occurs in the transition from immature to mature NK cells rather than earlier

• NK cells expressing Ly49A are reduced in the spleen and bone marrow

• in addition, NK populations in the bone marrow expressing CD11b^hi and CD43^hi are reduced as well as NK cells expressing Klrg1

• immature NK cells in the bone marrow have a higher proliferation rate and also have a faster differentiation and transition from the stage III to the more mature stage V

decreased B cell number ( J:151878 )

• T and B cell numbers are reduced in the spleen but not in other organs (i.e. lymph nodes, thymus, etc.)

decreased T cell number ( J:151878 )

• T and B cell numbers are reduced in the spleen but not in other organs (i.e. lymph nodes, thymus, etc.)

spleen hypoplasia ( J:151878 )

• spleen cellularity is reduced by a third to a half due to smaller numbers of T and B cells

• reduced lymphocytes numbers are restricted to the spleen as lymph node numbers are normal

abnormal NK cell physiology ( J:151878 )

• immature NK cells in the bone marrow have a higher proliferation rate

• NK cells are more susceptible to apoptosis in culture, and less responsive to the anti-apoptotic effects of IL-2 and IL-15

• NK cells produce more IFN-gamma than controls in response to Chinese Hamster Ovary cells, anti-NK1.1 stimulation, and to MCMV infection

impaired natural killer cell mediated cytotoxicity ( J:151878 )

• NK cells are less able to kill tumor cell lines expressing an NG2GD ligand

• NK cells are able to kill cells lacking MHC-I expression at the same efficiency as wild-type NK cells