Phenotypes associated with this allele

• Allele Symbol: Prickle1^tm1Nue
• Allele Name: targeted mutation 1, Naoto Ueno
• Allele ID: MGI:4356107
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prickle1^tm1Nue/Prickle1^tm1Nue	involves: C57BL/6 * CBA	MGI:4356108
ht2	Prickle1^tm1Nue/Prickle1^+	B6.Cg-Prickle1^tm1Nue	MGI:4941932
cx3	Prickle1^tm1Nue/Prickle1^+ Vangl2^Lp/Vangl2^+	involves: C57BL/6J * CBA * LPT/LeJ	MGI:4356109

Genotype
MGI:4356108

hm1

• Allelic Composition: Prickle1^tm1Nue/Prickle1^tm1Nue
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between somite formation and embryo turning, complete penetrance ( J:151993 )

• all die between E7.5 and E8.5

embryo

increased embryonic tissue cell apoptosis ( J:151993 )

• increase in apoptotic cells in the distal epiblast and visceral endoderm

abnormal anterior visceral endoderm cell migration ( J:151993 )

• data suggest that anterior to posterior cell movement does not occur in the proximal epiblast

• at E6.5, in the distal visceral endoderm cells positive for Hek, Dkk1, and Nodal fail to migrate toward the anterior

embryonic growth arrest ( J:151993 )

• arrest between E6.0 and E6.5

abnormal embryonic epiblast morphology ( J:151993 )

• the Wnt3 and T expression domains are expanded anteriorly

• nuclei in the epiblast are more spherical rather than ellipsoid and elongated as in controls

• the epiblast is disorganized with ectopic mitotic cells

• expression analysis indicates that apical-basal cell polarity is disrupted

absent mesoderm ( J:151993 )

failure of primitive streak formation ( J:151993 )

abnormal extraembryonic endoderm formation ( J:151993 )

• at E6.5, in the distal visceral endoderm cells positive for Hek, Dkk1, and Nodal fail to migrate toward the anterior

• at E7.5 Lefty1+ cells are dispersed rather than migrating as a cohort

• the visceral endoderm fails to form squamous cells in the distal egg cylinder

cellular

abnormal mitotic spindle morphology ( J:151993 )

• many mitotic spindles are oriented obliquely or parallel rather than perpendicular

decreased mitotic index ( J:151993 )

• on the apical side of the epiblast

• however, the overall mitotic index is only slightly lower than in controls

increased embryonic tissue cell apoptosis ( J:151993 )

• increase in apoptotic cells in the distal epiblast and visceral endoderm

abnormal anterior visceral endoderm cell migration ( J:151993 )

• data suggest that anterior to posterior cell movement does not occur in the proximal epiblast

• at E6.5, in the distal visceral endoderm cells positive for Hek, Dkk1, and Nodal fail to migrate toward the anterior

Genotype
MGI:4941932

ht2

• Allelic Composition: Prickle1^tm1Nue/Prickle1⁺
• Genetic Background: B6.Cg-Prickle1^tm1Nue

behavior/neurological

abnormal seizure response to electrical stimulation ( J:169136 )

• mice exhibit decreased seizure threshold in a maximal electroconvulsive seizure threshold test compared with wild-type mice

nervous system

abnormal seizure response to electrical stimulation ( J:169136 )

• mice exhibit decreased seizure threshold in a maximal electroconvulsive seizure threshold test compared with wild-type mice

Genotype
MGI:4356109

cx3

• Allelic Composition: Prickle1^tm1Nue/Prickle1⁺; Vangl2^Lp/Vangl2⁺
• Genetic Background: involves: C57BL/6J * CBA * LPT/LeJ

embryo

abnormal embryonic epiblast morphology ( J:151993 )

• nuclei in the epiblast are more spherical and randomly aligned along the apical basal axis

• expression analysis indicates that apical-basal cell polarity is disrupted similar to the phenotype of single homozygous Prickle1 mutant mice