Phenotypes associated with this allele

• Allele Symbol: Ebag9^tm1Rehm
• Allele Name: targeted mutation 1, Armin Rehm
• Allele ID: MGI:4359722
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ebag9^tm1Rehm/Ebag9^tm1Rehm	either: (involves: 129P2/OlaHsd * BALB/c) or (involves: 129P2/OlaHsd * C57BL/6)	MGI:4359735

Genotype
MGI:4359735

hm1

• Allelic Composition: Ebag9^tm1Rehm/Ebag9^tm1Rehm
• Genetic Background: either: (involves: 129P2/OlaHsd * BALB/c) or (involves: 129P2/OlaHsd * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Altered size of secretory lysosomes in Ebag9^tm1Rehm/Ebag9^tm1Rehm cytotoxic T lymphocytes

immune system

abnormal CD8-positive, alpha beta T cell morphology ( J:152534 )

• lytic granules in CD8 ⁺ T cells have a 30% decrease in volume

abnormal cytotoxic T cell physiology ( J:152534 )

• CD8 ⁺ T cells have 3-fold more cytolytic activity than controls in an allogenic cytotoxicity assay

• this enhanced cytotoxicity results from increased release of granzyme-containing lytic granules

• granzyme B is sorted to secretory lysosomes more efficiently than controls

• CTLs eliminate twice as many targets as controls when TAG-loaded splenocytes are transferred into mutant mice

• significantly lower Listeria monocytogenes titers are found after infection also indicating enhanced CTL activity

abnormal memory T cell physiology ( J:152534 )

• there is an enhanced secondary CD8 ⁺ T cell response to antigen loaded splenocytes after secondary challenge with antigen

decreased susceptibility to bacterial infection ( J:152534 )

• bacterial titers of Listeria monocytogenes are more than a log unit lower than controls 6 days after infection

hematopoietic system

abnormal CD8-positive, alpha beta T cell morphology ( J:152534 )

• lytic granules in CD8 ⁺ T cells have a 30% decrease in volume

abnormal cytotoxic T cell physiology ( J:152534 )

• CD8 ⁺ T cells have 3-fold more cytolytic activity than controls in an allogenic cytotoxicity assay

• this enhanced cytotoxicity results from increased release of granzyme-containing lytic granules

• granzyme B is sorted to secretory lysosomes more efficiently than controls

• CTLs eliminate twice as many targets as controls when TAG-loaded splenocytes are transferred into mutant mice

• significantly lower Listeria monocytogenes titers are found after infection also indicating enhanced CTL activity

abnormal memory T cell physiology ( J:152534 )

• there is an enhanced secondary CD8 ⁺ T cell response to antigen loaded splenocytes after secondary challenge with antigen