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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Nfil3tm1Look
targeted mutation 1, A Thomas Look
MGI:4361260
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Nfil3tm1Look/Nfil3tm1Look B6.129X1-Nfil3tm1Look MGI:4418329
hm2
 		Nfil3tm1Look/Nfil3tm1Look involves: 129X1/SvJ MGI:5576254
cx3
 		Il10tm1Cgn/Il10tm1Cgn
Nfil3tm1Look/Nfil3tm1Look 		involves: 129P2/OlaHsd * 129X1/SvJ MGI:5576253


Genotype
MGI:4418329
hm1
Allelic
Composition		 Nfil3tm1Look/Nfil3tm1Look
Genetic
Background		 B6.129X1-Nfil3tm1Look
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nfil3tm1Look mutation (0 available); any Nfil3 mutation (32 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal NK cell differentiation ( J:155677 )
• in vitro cultures of Lin- bone marrow cells fail to give rise to NK 1.1 expressing cells given the appropriate cytokine stiumuli
• analysis of in vivo NK cell development by flow cytometry shows development stalled at the iNK stage (NK1.1+ DX5-)
decreased NK cell number ( J:155677 )
• in the spleen, lung, and liver
impaired natural killer cell mediated cytotoxicity ( J:155677 )
• significantly impaired cytolytic activity against Yac-1 target cells in vitro
• unable to reject transferred MHC-class I deficient cells in vivo

immune system
abnormal NK cell differentiation ( J:155677 )
• in vitro cultures of Lin- bone marrow cells fail to give rise to NK 1.1 expressing cells given the appropriate cytokine stiumuli
• analysis of in vivo NK cell development by flow cytometry shows development stalled at the iNK stage (NK1.1+ DX5-)
decreased NK cell number ( J:155677 )
• in the spleen, lung, and liver
impaired natural killer cell mediated cytotoxicity ( J:155677 )
• significantly impaired cytolytic activity against Yac-1 target cells in vitro
• unable to reject transferred MHC-class I deficient cells in vivo
decreased interferon-gamma secretion ( J:155677 )
• decreased intracellular interferon-gamma production by NK cells




Genotype
MGI:5576254
hm2
Allelic
Composition		 Nfil3tm1Look/Nfil3tm1Look
Genetic
Background		 involves: 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nfil3tm1Look mutation (0 available); any Nfil3 mutation (32 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:209365 )
N
• mice do not develop colitis up to 6-8 months of age




Genotype
MGI:5576253
cx3
Allelic
Composition		 Il10tm1Cgn/Il10tm1Cgn
Nfil3tm1Look/Nfil3tm1Look
Genetic
Background		 involves: 129P2/OlaHsd * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il10tm1Cgn mutation (15 available); any Il10 mutation (45 available)
Nfil3tm1Look mutation (0 available); any Nfil3 mutation (32 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
colitis ( J:209365 )
• mice develop colitis
increased interleukin-12 secretion ( J:209365 )
• IL-12b (IL-12p40) production from activated macrophages is increased compared to single mutant

digestive/alimentary system
colitis ( J:209365 )
• mice develop colitis




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory