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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Obscntm1Chen
targeted mutation 1, Ju Chen
MGI:4361272
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Obscntm1Chen/Obscntm1Chen involves: 129 * Black Swiss * C57BL/6 * SJL MGI:6476961
hm2
Obscntm1Chen/Obscntm1Chen involves: 129S1/Sv * 129X1/SvJ MGI:4361274
cn3
Obscntm1Chen/Obscntm1Chen
Obsl1tm1.1Slan/Obsl1tm1.1Slan
Tg(Myog-cre)1Eno/0
involves: 129 * Black Swiss * C57BL/6 * SJL MGI:6476968


Genotype
MGI:6476961
hm1
Allelic
Composition
Obscntm1Chen/Obscntm1Chen
Genetic
Background
involves: 129 * Black Swiss * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Obscntm1Chen mutation (1 available); any Obscn mutation (420 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
muscle
N
• mice exhibit normal sarcomerogenesis in tibialis anterior (TA) muscles; Z-disc and M-band structures are not significantly altered
• at 4 months of age, muscle weight to tibia-length ratios for TA, soleus, gastrocnemius and extensor digitorum longus (EDL) muscles are normal
• no significant changes are noted in the number of centralized nuclei in TA muscle at 4 months of age
• analysis of subsarcolemmal dystrophin localization in longitudinal sections of TA muscles revealed a patchy and abnormal distribution of dystrophin, indicating increased membrane fragility even at baseline
• TA muscles show a decrease in protein levels of small ankyrin-1.5 (sAnk1.5, a binding partner for the obscurin-A C terminus) due to increased turnover of the protein
• analysis of cross-sectional areas in TA muscles revealed an increase in fiber size
• analysis of twitch parameters in EDL muscles revealed increased time-to-peak (TtP) values relative to control and Obsl1 skeletal muscle-knockouts
• however, half-relaxation times remain normal




Genotype
MGI:4361274
hm2
Allelic
Composition
Obscntm1Chen/Obscntm1Chen
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Obscntm1Chen mutation (1 available); any Obscn mutation (420 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
muscle
N
• skeletal muscle physiology are normal
• mice exhibit fewer fully extended longitudinal sarcoplasmic reticulum compared with wild-type mice
• mild in aged mice




Genotype
MGI:6476968
cn3
Allelic
Composition
Obscntm1Chen/Obscntm1Chen
Obsl1tm1.1Slan/Obsl1tm1.1Slan
Tg(Myog-cre)1Eno/0
Genetic
Background
involves: 129 * Black Swiss * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Obscntm1Chen mutation (1 available); any Obscn mutation (420 available)
Obsl1tm1.1Slan mutation (0 available); any Obsl1 mutation (100 available)
Tg(Myog-cre)1Eno mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• mice are viable and develop normally

muscle
N
• analysis of myofilament structure using sarcomeric alpha-actinin 2 indicated normal sarcomerogenesis in tibialis anterior (TA) muscles
• immunofluorescence analysis of sarcomeric alpha-actinin 2 and titin-M8 localization revealed that Z-disc and M-band structures are not significantly altered
• at 4 months of age, muscle weight to tibia-length ratios for TA, soleus, gastrocnemius and extensor digitorum longus (EDL) muscles are normal
• no significant changes are observed in the number of centralized nuclei in TA muscle at 4 months of age
• skeletal muscles show a slight decrease in protein levels of utrophin and other dystrophin-sarcoglycan (DSG) components, including alpha-dystrobrevin, whereas levels of tubulin are slightly increased
• although overall dystrophin levels are normal, subsarcolemmal dystrophin localization in longitudinal sections of TA muscles is more patchy and abnormally distributed than in single Obscntm1Chen knockouts, indicating exacerbated membrane fragility
• a significant portion of TA muscle fibers are mouse IgG-positive, unlike in single Obscntm1Chen knockouts or Obsl1 skeletal muscle-knockouts, suggesting impaired sarcolemmal integrity
• increased membrane fragility leads to upregulation of dysferlin and filamin C proteins (involved in muscle repair), reduced caveolin-1 and TRPC1 protein levels, but normal levels of neuronal nitric oxidase (nNOS) and L-type calcium channel DHPR alpha-2 subunit, indicating potential changes to DSG-associated, but not T-tubule-based ion channels
• TA muscles show a decrease in small ankyrin-1.5 (sAnk1.5) protein levels similar to that in single Obscntm1Chen knockouts
• skeletal muscles show alterations to levels of lumenal SR calcium binding proteins (sarcalumenin and Casq2) as well as sarcoendoplasmic reticulum Ca2+ ATPase (Serca)
• proteome and expression data revealed increases in junctional SR proteins like triadin, junction and ryanodine receptors
• analysis of cross-sectional areas of TA muscle revealed a decrease in fiber size
• analysis of twitch parameters in EDL muscles revealed increased time-to-peak (TtP) values relative to control and Obsl1 skeletal muscle-knockouts
• however, half-relaxation times remain normal

homeostasis/metabolism
• monoamine oxidase A and B levels are significantly decreased in TA muscles
• catalase levels are significantly increased in soleus muscle
• muscle glycogen phosphorylase, the rate determining enzyme responsible for glycogen breakdown, is significantly decreased

cellular
• almost all identified mitochondrial electron transport chain proteins are downregulated in soleus and TA muscles
• analysis of whole TA and/or soleus muscle extracts using oxphos antibody cocktail or a Uqcrb antibody revealed slightly or significantly reduced levels of mitochondrial complex I, II, III, IV, and V proteins

reproductive system
N
• mice are fertile





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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory