Phenotypes associated with this allele

• Allele Symbol: Ins2^tm1Mnt
• Allele Name: targeted mutation 1, Massimo Trucco
• Allele ID: MGI:4361633
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ins1^tm1Jja/Ins1^tm1Jja Ins2^tm1Mnt/Ins2^tm1Mnt Tg(Aire-cre)1Mnt/0	involves: 129S2/SvPas * 129S4/SvJaeSor * C57BL/6 * FVB/N	MGI:4361713

Genotype
MGI:4361713

cn1

• Allelic Composition: Ins1^tm1Jja/Ins1^tm1Jja; Ins2^tm1Mnt/Ins2^tm1Mnt; Tg(Aire-cre)1Mnt/0
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJaeSor * C57BL/6 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

decreased pancreatic beta cell number ( J:152798 )

• by 4 weeks after birth, few insulin positive beta-cells are present in pancreata

abnormal pancreatic beta cell physiology ( J:152798 )

• function is impaired as indicated by lower circulating insulin levels

pancreas inflammation ( J:152798 )

• islets in pancreata of diabetic mice contain CD8+ and CD4+ T cells, B-cells, and macrophages at 3 weeks

homeostasis/metabolism

increased circulating glucose level ( J:152798 )

• after postnatal day 10, mice begin to develop elevated blood sugar level compare to controls

hyperglycemia ( J:152798 )

• by 3 weeks of age, both males and females exhibit severe hyperglycemia

abnormal circulating insulin level ( J:152798 )

• levels are significantly lower at 3 weeks compared to controls

immune system

abnormal immune system physiology ( J:152798 )

• mice show impaired immune tolerance towards insulin; tolerance towards other autoantigens is unimpaired

pancreas inflammation ( J:152798 )

• islets in pancreata of diabetic mice contain CD8+ and CD4+ T cells, B-cells, and macrophages at 3 weeks

abnormal T cell physiology ( J:152798 )

• upon stimulation with insulin, interferon-gamma secreting T cells are detected;

• pancreata of nude mice that received transplants of thymi from diabetic mutants display insulitis with T cell infiltrates at 16 weeks after transplantation

• Rag1 mice that received transfer of splenocytes from diabetic mutants show elevated blood glucose at 1-2 weeks after transfer with autoreactive T cells detected; at 4 weeks after transfer of CD4+ or CD8+ T cells, pancreata of Rag1 mice show insulitis and beta-cell destruction

abnormal interferon-gamma secretion ( J:152798 )

• insulin-stimulated T cells show high production of interferon-gamma

increased anti-insulin autoantibody level ( J:152798 )

• levels of anti-insulin autoantibodies in sera of 3-8 week old mice are significantly higher than in controls

hematopoietic system

abnormal T cell physiology ( J:152798 )

• upon stimulation with insulin, interferon-gamma secreting T cells are detected;

• pancreata of nude mice that received transplants of thymi from diabetic mutants display insulitis with T cell infiltrates at 16 weeks after transplantation

• Rag1 mice that received transfer of splenocytes from diabetic mutants show elevated blood glucose at 1-2 weeks after transfer with autoreactive T cells detected; at 4 weeks after transfer of CD4+ or CD8+ T cells, pancreata of Rag1 mice show insulitis and beta-cell destruction