Phenotypes associated with this allele

• Allele Symbol: Hsd11b1^{tm1a(KOMP)Wtsi}
• Allele Name: targeted mutation 1a, Wellcome Trust Sanger Institute
• Allele ID: MGI:4362580
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hsd11b1^tm1a(KOMP)Wtsi/Hsd11b1^tm1a(KOMP)Wtsi	involves: C57BL/6Jcl * C57BL/6N	MGI:5698871
ht2	Hsd11b1^tm1a(KOMP)Wtsi/Hsd11b1^+	involves: C57BL/6Jcl * C57BL/6N	MGI:5698872

Genotype
MGI:5698871

hm1

• Allelic Composition: Hsd11b1^{tm1a(KOMP)Wtsi}/Hsd11b1^{tm1a(KOMP)Wtsi}
• Genetic Background: involves: C57BL/6Jcl * C57BL/6N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

abnormal corticosterone level ( J:214146 )

• at 1 year of age, homozygotes display a significant decrease in skin corticosterone levels relative to wild-type controls, while plasma corticosterone levels are relatively normal

increased circulating corticosterone level ( J:214146 )

• at 3 months of age, homozygotes display increased plasma corticosterone levels relative to wild-type controls

• however, no significant differences in plasma corticosterone levels are detected at 1 year of age

decreased collagen level ( J:214146 )

• expression of Col1A1 is significantly decreased while expression of MMP-13 is significantly increased in homozygous mutant dermal fibroblasts relative to wild-type controls, suggesting decreased collagen production and increased collagen degradation

• at 1 year of age, homozygotes display decreased collagen type 1 (Col1A1 and Col1A2) expression relative to wild-type controls

• however, no significant differences in collagen type 1 expression are detected at 3 months of age

cellular

abnormal fibroblast proliferation ( J:214146 )

• in response to a 3-day treatment with a selective 11beta-HSD1 inhibitor (10 uM), cell proliferation is mildly enhanced in wild-type dermal fibroblasts but not in homozygous mutant mouse fibroblasts relative to vehicle-treated controls

increased fibroblast proliferation ( J:214146 )

• on days 2 and 3, in vitro proliferation of dermal fibroblasts derived from homozygous mutant mice is mildly increased relative to wild-type controls, as shown by an MTS assay

integument

increased subcutaneous adipose tissue amount ( J:214146 )

• at 1 year of age, homozygotes show a slight increase in subcutaneous adipose tissue relative to wild-type controls

• however, no significant differences in dermal thickness are noted at 3 months or 1 year of age

adipose tissue

increased subcutaneous adipose tissue amount ( J:214146 )

• at 1 year of age, homozygotes show a slight increase in subcutaneous adipose tissue relative to wild-type controls

• however, no significant differences in dermal thickness are noted at 3 months or 1 year of age

Genotype
MGI:5698872

ht2

• Allelic Composition: Hsd11b1^{tm1a(KOMP)Wtsi}/Hsd11b1⁺
• Genetic Background: involves: C57BL/6Jcl * C57BL/6N

homeostasis/metabolism

abnormal corticosterone level ( J:214146 )

• at 1 year of age, heterozygotes show a significant decrease in skin corticosterone levels relative to wild-type controls, while plasma corticosterone levels are relatively normal

increased circulating corticosterone level ( J:214146 )

• at 3 months of age, heterozygotes display increased plasma corticosterone levels relative to wild-type controls

• however, no significant differences in plasma corticosterone levels are detected at 1 year of age

decreased collagen level ( J:214146 )

• expression of Col1A1 is significantly decreased while expression of MMP-13 is significantly increased in heterozygous mutant dermal fibroblasts relative to wild-type controls, suggesting decreased collagen production and increased collagen degradation

• however, no significant differences in dermal thickness or collagen type 1 (Col1A1 and Col1A2) expression are detected at 3 months or 1 year of age relative to wild-type controls

cellular

increased fibroblast proliferation ( J:214146 )

• on day 3, in vitro proliferation of dermal fibroblasts derived from heterozygous mutant mice is mildly increased relative to wild-type controls, as shown by an MTS assay