Phenotypes associated with this allele

• Allele Symbol: Miga2^{tm1a(KOMP)Wtsi}
• Allele Name: targeted mutation 1a, Wellcome Trust Sanger Institute
• Allele ID: MGI:4362858
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Miga2^tm1a(KOMP)Wtsi/Miga2^tm1a(KOMP)Wtsi	B6JTyr;B6N-Miga2^tm1a(KOMP)Wtsi/Wtsi	MGI:5781627
hm2	Miga2^tm1a(KOMP)Wtsi/Miga2^tm1a(KOMP)Wtsi	C57BL/6N-Miga2^tm1a(KOMP)Wtsi/Mmucd	MGI:7716931
cx3	Irf1^tm1Mak/Irf1^tm1Mak Miga2^tm1a(KOMP)Wtsi/Miga2^tm1a(KOMP)Wtsi	B6.Cg-Miga2^tm1a(KOMP)Wtsi Irf1^tm1Mak	MGI:7716957
cx4	Miga2^tm1a(KOMP)Wtsi/Miga2^tm1a(KOMP)Wtsi Pnp2^em1Smoc/Pnp2^em1Smoc	C57BL/6-Miga2^tm1a(KOMP)Wtsi Pnp2^em1Smoc	MGI:7716953

Genotype
MGI:5781627

hm1

• Allelic Composition: Miga2^{tm1a(KOMP)Wtsi}/Miga2^{tm1a(KOMP)Wtsi}
• Genetic Background: B6JTyr;B6N-Miga2^{tm1a(KOMP)Wtsi}/Wtsi
• Cell Lines: EPD0026_1_C12

Data Sources

IMPC Data for Miga2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

decreased total body fat amount ( J:175295 )

IMPC - WTSI

behavior/neurological

abnormal behavior ( J:175295 )

IMPC - WTSI

hyperactivity ( J:175295 )

IMPC - WTSI

craniofacial

abnormal cranium morphology ( J:175295 )

♂

IMPC - WTSI

abnormal tooth morphology ( J:175295 )

♂

IMPC - WTSI

abnormal maxilla morphology ( J:175295 )

♂

IMPC - WTSI

abnormal snout morphology ( J:175295 )

♂

IMPC - WTSI

growth/size/body

abnormal tooth morphology ( J:175295 )

♂

IMPC - WTSI

abnormal snout morphology ( J:175295 )

♂

IMPC - WTSI

decreased lean body mass ( J:175295 )

IMPC - WTSI

decreased body weight ( J:175295 )

IMPC - WTSI

decreased body length ( J:175295 )

IMPC - WTSI

hematopoietic system

decreased hematocrit ( J:175295 )

♂

IMPC - WTSI

decreased hemoglobin content ( J:175295 )

♂

IMPC - WTSI

homeostasis/metabolism

decreased circulating cholesterol level ( J:175295 )

IMPC - WTSI

decreased circulating HDL cholesterol level ( J:175295 )

IMPC - WTSI

decreased circulating LDL cholesterol level ( J:175295 )

IMPC - WTSI

decreased circulating aspartate transaminase level ( J:175295 )

IMPC - WTSI

decreased circulating serum albumin level ( J:175295 )

IMPC - WTSI

decreased circulating total protein level ( J:175295 )

IMPC - WTSI

decreased lactate dehydrogenase level ( J:175295 )

♂

IMPC - WTSI

skeleton

abnormal cranium morphology ( J:175295 )

♂

IMPC - WTSI

abnormal tooth morphology ( J:175295 )

♂

IMPC - WTSI

abnormal maxilla morphology ( J:175295 )

♂

IMPC - WTSI

decreased bone mineral content ( J:175295 )

IMPC - WTSI

decreased bone mineral density ( J:175295 )

♀

IMPC - WTSI

Genotype
MGI:7716931

hm2

• Allelic Composition: Miga2^{tm1a(KOMP)Wtsi}/Miga2^{tm1a(KOMP)Wtsi}
• Genetic Background: C57BL/6N-Miga2^{tm1a(KOMP)Wtsi}/Mmucd
• Cell Lines: EPD0026_1_C12

behavior/neurological

increased depression-related behavior ( J:339075 )

• mice stop struggling earlier in the tail suspension test, indicating depression-like behavior

increased anxiety-related response ( J:339075 )

• mice display decreased locomotor activity and spend much less time in the center of the open-field test

• mice are afraid to enter into the open arms in the elevated plus maze test

• in the dark-light transition test, mice exhibit fewer transitions from the dark zone to the light zone and spend less time in the light zone

• treatment of mice with VLA-4 or CD6 neutralizing antibodies has no effect on anxiety symptom

increased thigmotaxis ( J:339075 )

• mice spend much less time in the center of the open-field test

decreased social novelty preference ( J:339075 )

• in the social novelty task, mice spend reduced contact time with a stranger mouse, indicating less motivation and curiosity

hematopoietic system

abnormal CD4-positive, alpha beta T cell morphology ( J:339075 )

• naive CD4+ T cells exhibit highly fragmented mitochondria

• many small, diverse mitochondria dispersed in the cytoplasm of naive CD4+ T cells

immune system

abnormal CD4-positive, alpha beta T cell morphology ( J:339075 )

• naive CD4+ T cells exhibit highly fragmented mitochondria

• many small, diverse mitochondria dispersed in the cytoplasm of naive CD4+ T cells

Genotype
MGI:7716957

cx3

• Allelic Composition: Irf1^tm1Mak/Irf1^tm1Mak; Miga2^{tm1a(KOMP)Wtsi}/Miga2^{tm1a(KOMP)Wtsi}
• Genetic Background: B6.Cg-Miga2^{tm1a(KOMP)Wtsi} Irf1^tm1Mak

behavior/neurological

behavior/neurological phenotype ( J:339075 )

N • mice show restoration of most of the anxiety-like phenotypes seen in single Miga2^{tm1a(KOMP)Wtsi} homozygotes

Genotype
MGI:7716953

cx4

• Allelic Composition: Miga2^{tm1a(KOMP)Wtsi}/Miga2^{tm1a(KOMP)Wtsi}; Pnp2^em1Smoc/Pnp2^em1Smoc
• Genetic Background: C57BL/6-Miga2^{tm1a(KOMP)Wtsi} Pnp2^em1Smoc

hematopoietic system

abnormal CD4-positive, alpha-beta T cell physiology ( J:339075 )

• CD4+ T cells grown in culture show partial normalization of the hyperproduction of xanthine that is seen in single Miga2^{tm1a(KOMP)Wtsi} homozygotes but still greater than in wild-type mice

immune system

abnormal CD4-positive, alpha-beta T cell physiology ( J:339075 )

• CD4+ T cells grown in culture show partial normalization of the hyperproduction of xanthine that is seen in single Miga2^{tm1a(KOMP)Wtsi} homozygotes but still greater than in wild-type mice