All Phenotypes Usp22<tm1a(KOMP)Wtsi> MGI Mouse

mortality/aging ( J:233975 )

N	• contrary to expectation, homozygotes are born with the expected Mendelian frequency and are viable as adults

decreased body weight ( J:233975 )

• at 4 months of age, body weight is reduced by 40% relative to controls

postnatal growth retardation ( J:233975 )

• at 4 months of age, mice exhibit growth retardation relative to wild-type and heterozygous controls

abnormal intestinal enteroendocrine cell morphology ( J:233975 )

• adult mice exhibit ~50% and ~45% more chromogranin A (CGA)-positive enteroendocrine cells in the crypts and villi of the small intestine, respectively, relative to controls

abnormal intestinal epithelium morphology ( J:233975 )

• adult mice show impaired cell differentiation in the small intestine epithelium

abnormal small intestine morphology ( J:233975 )

• at 4 months of age, mice show a slight increase in the number of olfactomedin-positive stem cells located near the crypt base of the small intestine relative to controls

• detailed analysis of cellular composition revealed an increase of differentiated cell types in the small intestine

• however, gross morphology is normal with respect to villi length, number of crypts, and number of cells per crypt

abnormal intestinal villus goblet cell morphology ( J:233975 )

• adult mice show a ~2-fold increase in the number of MUC2-positive goblet cells in the villi of the small intestine relative to controls

• however, the number of goblet cells in the crypts of the small intestine are normal

increased Paneth cell number ( J:233975 )

• adult mice show an increased number of lysozyme (LYZ)-positive Paneth cells in the crypts of the small intestine (4-5 cells per crypt) relative to controls (3 cells per crypt)

impaired neuron differentiation ( J:233975 )

• adult mice exhibit impaired neuron differentiation in the cerebral cortex

abnormal cortical plate morphology ( J:233975 )

• at 4 months of age, later born (SATB2-positive) neurons appear more scattered within the cortical plate

abnormal cerebral cortex morphology ( J:233975 )

• adult mice display a smaller and less densely packed cerebral cortex relative to controls

• immunohistochemistry revealed less differentiated cortical cells and impaired layering on coronal sections

• however, the localization and distribution of intermediate precursor cells (IPC) in the subventricular zone is normal

abnormal stratification in cerebral cortex ( J:233975 )

• at 4 months of age, layering of the deep-layer (CTIP2-positive, layer V-VI) and upper-layer glutamatergic neurons (SATB2-positive, layer II-IV) is impaired relative to controls