Allele Symbol Allele Name Allele ID |
Usp22tm1a(KOMP)Wtsi targeted mutation 1a, Wellcome Trust Sanger Institute MGI:4364127 |
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Summary |
3 genotypes
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Data Sources
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♀ | phenotype observed in females |
♂ | phenotype observed in males |
N | normal phenotype |
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♀ | phenotype observed in females |
♂ | phenotype observed in males |
N | normal phenotype |
N |
• contrary to expectation, homozygotes are born with the expected Mendelian frequency and are viable as adults
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• at 4 months of age, body weight is reduced by 40% relative to controls
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• at 4 months of age, mice exhibit growth retardation relative to wild-type and heterozygous controls
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• adult mice exhibit ~50% and ~45% more chromogranin A (CGA)-positive enteroendocrine cells in the crypts and villi of the small intestine, respectively, relative to controls
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• adult mice show impaired cell differentiation in the small intestine epithelium
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• at 4 months of age, mice show a slight increase in the number of olfactomedin-positive stem cells located near the crypt base of the small intestine relative to controls
• detailed analysis of cellular composition revealed an increase of differentiated cell types in the small intestine
• however, gross morphology is normal with respect to villi length, number of crypts, and number of cells per crypt
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• adult mice show a ~2-fold increase in the number of MUC2-positive goblet cells in the villi of the small intestine relative to controls
• however, the number of goblet cells in the crypts of the small intestine are normal
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• adult mice show an increased number of lysozyme (LYZ)-positive Paneth cells in the crypts of the small intestine (4-5 cells per crypt) relative to controls (3 cells per crypt)
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• adult mice exhibit impaired neuron differentiation in the cerebral cortex
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• at 4 months of age, later born (SATB2-positive) neurons appear more scattered within the cortical plate
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• adult mice display a smaller and less densely packed cerebral cortex relative to controls
• immunohistochemistry revealed less differentiated cortical cells and impaired layering on coronal sections
• however, the localization and distribution of intermediate precursor cells (IPC) in the subventricular zone is normal
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• at 4 months of age, layering of the deep-layer (CTIP2-positive, layer V-VI) and upper-layer glutamatergic neurons (SATB2-positive, layer II-IV) is impaired relative to controls
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• adult mice show a ~2-fold increase in the number of MUC2-positive goblet cells in the villi of the small intestine relative to controls
• however, the number of goblet cells in the crypts of the small intestine are normal
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• adult mice exhibit impaired cell differentiation in the small intestine epithelium and in neurons in the cerebral cortex
• however, levels of histone H2B and monoubiquitination of histone H2B (H2Bub1) remain constant in protein samples isolated from mutant tissues
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• adult mice exhibit impaired neuron differentiation in the cerebral cortex
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• adult mice show an increased number of lysozyme (LYZ)-positive Paneth cells in the crypts of the small intestine (4-5 cells per crypt) relative to controls (3 cells per crypt)
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Data Sources
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♀ | phenotype observed in females |
♂ | phenotype observed in males |
N | normal phenotype |
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IMPC - WTSI
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IMPC - WTSI
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IMPC - WTSI
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IMPC - WTSI
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 08/21/2024 MGI 6.24 |
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