All Phenotypes Mfap2<tm1a(KOMP)Wtsi> MGI Mouse

abnormal bone marrow adipose tissue morphology ( J:236092 )

• by 10 months of age, homozygotes show 5-fold increase in bone marrow osmium-stained lipid volume within the decalcified proximal tibia relative to wild-type controls, indicating pathological marrow adipose tissue (MAT) expansion

• however, bone marrow adiposity is normal at 2 and 6 months of age, and bone marrow cellularity is not significantly reduced at 10 months of age despite MAT expansion

increased bone marrow adipose tissue amount ( J:236092 )

• by 10 months of age, homozygotes exhibit a 5-fold increase in bone marrow fat volume relative to wild-type controls

• however, MAT expansion is not coincident with bone loss or excess peripheral adiposity

decreased bone mineral density ( J:236092 )

• at 2 and 6 months of age, homozygotes show a significant reduction in trabecular bone mineral density (BMD) relative to wild-type controls

• however, no significant differences are noted in trabecular BMD at 10 months of age

• cortical BMD is normal at all ages and unchanged by MAT expansion

decreased trabecular bone volume ( J:236092 )

• at 2 and 6 months of age, homozygotes show a significant reduction in the ratio of trabecular bone volume to total volume (BV/TV) relative to wild-type controls

• however, this phenotype does not progress with age or MAT expansion

abnormal bone marrow adipose tissue morphology ( J:236092 )

• by 10 months of age, homozygotes show 5-fold increase in bone marrow osmium-stained lipid volume within the decalcified proximal tibia relative to wild-type controls, indicating pathological marrow adipose tissue (MAT) expansion

• however, bone marrow adiposity is normal at 2 and 6 months of age, and bone marrow cellularity is not significantly reduced at 10 months of age despite MAT expansion

increased bone marrow adipose tissue amount ( J:236092 )

• by 10 months of age, homozygotes exhibit a 5-fold increase in bone marrow fat volume relative to wild-type controls

• however, MAT expansion is not coincident with bone loss or excess peripheral adiposity

increased gonadal fat pad weight ( J:236092 )

• at 2 months of age, gonadal fat pad mass is only modestly increased by 15% relative to wild-type controls

• gonadal fat pad mass is increased by 2.7-fold at 6 months and by 1.8-fold at 10 months of age, indicating that the differential in fat mass stabilizes with age

increased body weight ( J:236092 )

• although normal at 2 months of age, total body weight is significantly increased by 17% at both 6 and 10 months of age relative to wild-type controls, indicating that the weight differential stabilizes with age

increased fasting circulating glucose level ( J:236092 )

• at 2 months of age, 6-hour fasting blood glucose levels are increased by 12% relative to wild-type controls

• at 6 months of age, fasted blood glucose levels are 63% higher than in wild-type controls

• by 10 months of age, fasted blood glucose levels are only 35% higher than in wild-type controls

hyperglycemia ( J:236092 )

• homozygotes are severely hyperglycemic by 6 months of age

insulin resistance ( J:236092 )

• homozygotes develop insulin resistance by 10 months of age, coincident with bone marrow adipose tissue expansion

abnormal hemopoiesis ( J:236092 )

• at 10 months of age, homozygotes show modest alterations in basal hematopoiesis, including a shift from granulopoiesis to B lymphopoiesis

increased myeloid dendritic cell number ( J:236092 )

• at 10 months of age, myeloid-derived dendritic cell numbers are modestly increased in the bone marrow (+78%) and significantly increased in the spleen (+250%) relative to wild-type controls

increased B cell number ( J:236092 )

• at 10 months of age, B cell numbers are modestly increased in the bone marrow (+18%) relative to wild-type controls; a trend toward increased B-cells is noted in spleen

increased macrophage cell number ( J:236092 )

• at 10 months of age, macrophage cell numbers are modestly increased in the bone marrow (+61%) relative to wild-type controls

increased monocyte cell number ( J:236092 )

• at 10 months of age, blood monocyte numbers are significantly increased (+216%) relative to wild-type controls

decreased leukocyte cell number ( J:236092 )

• at 10 months of age, homozygotes show a significant decrease in the number of circulating WBCs relative to wild-type controls

decreased neutrophil cell number ( J:236092 )

• at 10 months of age, neutrophil cell numbers are modestly decreased in the bone marrow (-13%) and significantly reduced in spleen (-72%) relative to wild-type controls

decreased B cell number ( J:236092 )

• at 10 months of age, blood B cell numbers are modestly decreased (-15%) relative to wild-type controls

increased splenocyte number ( J:236092 )

• at 10 months of age, homozygotes show a slight increase in spleen cellularity relative to wild-type controls