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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Cyth3tm2a(KOMP)Wtsi
targeted mutation 2a, Wellcome Trust Sanger Institute
MGI:4365249
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Cyth3tm2a(KOMP)Wtsi/Cyth3tm2a(KOMP)Wtsi C57BL/6N-Cyth3tm2a(KOMP)Wtsi MGI:6362991


Genotype
MGI:6362991
hm1
Allelic
Composition
Cyth3tm2a(KOMP)Wtsi/Cyth3tm2a(KOMP)Wtsi
Genetic
Background
C57BL/6N-Cyth3tm2a(KOMP)Wtsi
Cell Lines EPD0064_1_E05
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cyth3tm2a(KOMP)Wtsi mutation (1 available); any Cyth3 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
N
• on a chow diet, young adult (7-12 weeks old) male and female show normal total body weight and organ weights relative to wild-type controls
• aged mice are generally leaner than wild-type mice
• at >21 weeks of age, body weight of males is significantly lower than that in wild-type controls
• however, body weight is not due to reduced body size, as determined by tibia length of young adult mice
• chow-fed males show a reduction in age-induced weight gain with no significant changes in inguinal or gonadal WAT mass (WATi + 6% and WATg +19%) relative to wild-type controls (WATi + 28% and WATg +102%) at 2 years of age
• following a high-fat diet (HFD, 60 kJ% fat) for 6 weeks, young adult males show a significantly lower body weight gain and fat mass than HFD-fed wild-type controls; however, lean mass is unaffected

liver/biliary system
• at 2 years of age, liver glycogen levels of males are 50% lower than those in age-matched wild-type controls
• on a normal-fat control diet (CD, 13 kJ% fa), liver glycogen levels of males are significantly lower than those in CD-fed wild-type controls
• following a HFD for 6 weeks, young adult males fail to show a significant reduction of the already low liver glycogen levels, unlike HFD-fed wild-type controls
• following a HFD for 6 weeks, young adult males fail to show an increase in liver lipid content and liver triglyceride levels are significantly lower than those in HFD-fed wild-type controls
• after i.p. insulin injection of chow-fed young adult mice, livers show a significantly lower activation of the insulin receptor (IR)-downstream targets AKT and ERK1/2 relative to wild-type controls; however, phosphorylation of IRbeta is normal
• glucokinase (Gck) expression is significantly reduced in livers from mice fed ad libitum as well as in livers from mice re-fed following a starvation period
• Pepck gene expression is not significantly induced in livers following starvation due to an already high expression of Pepck with food ad libitum; furthermore, repression by re-feeding is significantly lower than that in wild-type controls

adipose tissue
• at 2 years of age, mice show a significantly smaller increase in gonadal WAT mass (WATg +19%) relative to wild-type controls (WATg +102%)
• following a HFD for 6 weeks, young adult males show a significantly lower gonadal fat pad weight than HFD-fed wild-type controls
• at 2 years of age, mice show a significantly smaller increase in inguinal WAT mass (WATi + 6%) relative to wild-type controls (WATi + 28%)
• following a HFD for 6 weeks, young adult males show a significantly lower inguinal fat pad weight than HFD-fed wild-type controls
• after i.p. insulin injection of chow-fed young adult mice, subcutaneous inguinal white adipose tissue (WATi) shows a 40-50% reduction in AKT and ERK1/2 activation relative to wild-type controls
• at 2 years of age, expression of Pnpla2 (an insulin dependent triglyceride lipase in adipose tissue), is significantly increased in gonadal WAT (WATg), indicating reduced insulin sensitivity

homeostasis/metabolism
N
• on a chow diet, young adult (7-12 weeks old) male and female mice show no overt signs of diabetes, with no differences in non-fasted blood glucose levels, serum levels of insulin, glucagon, triglycerides, leptin and liver glycogen relative to wild-type controls
• despite reduced IR-signaling in liver and adipose tissue, young adult mice are both glucose tolerant and insulin tolerant with no significant differences in serum levels of insulin and glucagon following starvation or re-feeding relative to wild-type controls
• aged (2-yr-old) mice show normal serum levels of insulin, glucagon, triglycerides, and leptin relative to wild-type controls
• following a HFD for 6 weeks, young adult males show a normal metabolic rate, with no significant changes in oxygen consumption, CO2 production, energy expenditure or body temperature relative to HFD-fed wild-type controls
• HFD-fed and aged mice show significantly increased lipid secretion induced by decreased bile acid synthesis
• following a high-fat diet (HFD, 60 kJ% fat) for 6 weeks, young adult males show a significantly lower body weight gain and fat mass than HFD-fed wild-type controls; however, lean mass is unaffected
• at 2 years of age, blood glucose levels of males are significantly lower than those in wild-type controls
• on a CD and HDF diet, blood glucose levels of males are 11% (CD) and 13% (HFD) lower than those in wild-type controls
• after 4 hrs of re-feeding, young adult mice show significantly lower serum triglyceride levels than re-fed wild-type controls
• at 2 years of age, liver glycogen levels of males are 50% lower than those in age-matched wild-type controls
• on a normal-fat control diet (CD, 13 kJ% fa), liver glycogen levels of males are significantly lower than those in CD-fed wild-type controls
• following a HFD for 6 weeks, young adult males fail to show a significant reduction of the already low liver glycogen levels, unlike HFD-fed wild-type controls
• following a HFD for 6 weeks, young adult males develop insulin resistance but are significantly lighter than HFD-fed wild-type controls
• on a CD diet, glucose clearance is significantly reduced 10 min after insulin administration compared to CD-fed wild-type controls
• HFD-fed and aged males show a significantly lower expression of bile acid synthetic enzymes (Cyp8b1 and Cyp27a1) relative to wild-type controls
• following a HFD for 6 weeks, young adult males fail to show an increase in liver lipid content and liver triglyceride levels are significantly lower than those in HFD-fed wild-type controls
• neither Glut4 nor Fasn (genes coding for de novo lipogenesis) are significantly up-regulated in WATi of young adult mice after re-feeding, unlike in wild-type controls; Srebp1c expression in WATi is by trend lower than that in wild-type controls after re-feeding

digestive/alimentary system
• HFD-fed and aged mice show significantly increased lipid secretion induced by decreased bile acid synthesis

behavior/neurological
N
• mice fed a CD or a HFD diet show normal food intake and locomotor activity in metabolic cages relative to similarly fed wild-type controls





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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory