Analysis Tools
mortality/aging
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• mice die between E10.5 and E14.5
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• mice die between E10.5 and E14.5
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cardiovascular system
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• between E10.5 and E14.5, mice exhibit cardiovascular abnormalities including aberrant vascular beds with dilated blood vessels and pericardial effusion unlike wild-type mice
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• at E10.5, pericytes stretch away from the endothelium and only cover 23% of the endothelial area compared with 58% in wild-type mice
• at E10.5, pericytes fail to reach the growing vascular front unlike in wild-type mice
• at E13.5, pericytes are round instead of spread as in wild-type mice
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• yolk sac and placenta blood vessels exhibit poor mural cell coverage and impaired mural cell to endothelial cell association unlike in wild-type mice
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• mice exhibit increased sprouting in the embryonic vascular plexus compared with wild-type mice
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• yolk sac and placenta blood vessels exhibit poor mural cell coverage and impaired mural cell to endothelial cell association unlike in wild-type mice
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• at E13.5, smooth muscle actin positive cells from yolk sacs are round instead of spread as in wild-type mice
• in culture, smooth muscle actin positive cells from the yolk sac fail to spread and do not develop a single broad lamella unlike similarly treated wild-type cells
• in culture, smooth muscle actin positive cells exhibit accelerated rates of random chemokinetic migration but reduced migration efficiency in response to PDGF-BB compared with similarly treated wild-type cells
• in culture, smooth muscle actin positive cells exhibit high contractility with loss of persistent and directed migration compared with similarly treated wild-type cells
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• between E10.5 and E14.5 associated with reduced mural cell coverage
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• at E12.5, septation of the truncus arteriosus is defective leading to persistent single outflow tract
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• at E12.5 and E13.5, the number of cardiomyofibrils is decreased compared to in wild-type mice
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• at E12.5 and E13.5, cardiomyocytes are round rather than elongated as in wild-type mice
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• at E12.5 and E13.5, cardiomyocytes are distributed in a random pattern
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• between E10.5 and E14.5
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• between E10.5 and E14.5, mice exhibit multiple microaneurysms unlike wild-type mice
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hemorrhage
(
J:154437
)
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• by E12.5 due to ruptured blood vessels
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embryo
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• yolk sac and placenta blood vessels exhibit poor mural cell coverage and impaired mural cell to endothelial cell association unlike in wild-type mice
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• yolk sac and placenta blood vessels exhibit poor mural cell coverage and impaired mural cell to endothelial cell association unlike in wild-type mice
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• beginning at E10.5
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renal/urinary system
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• mice exhibit severe kidney agenesis/dysgenesis unlike wild-type mice
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• in some mice
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• at E12.5 and E13.5, ureteric branches are absent unlike in wild-type mice
• however, ureteric bud invasion of metanephros mesenchyme is normal
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growth/size/body
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• beginning at E10.5
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homeostasis/metabolism
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• between E10.5 and E14.5
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muscle
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• at E13.5, smooth muscle actin positive cells from yolk sacs are round instead of spread as in wild-type mice
• in culture, smooth muscle actin positive cells from the yolk sac fail to spread and do not develop a single broad lamella unlike similarly treated wild-type cells
• in culture, smooth muscle actin positive cells exhibit accelerated rates of random chemokinetic migration but reduced migration efficiency in response to PDGF-BB compared with similarly treated wild-type cells
• in culture, smooth muscle actin positive cells exhibit high contractility with loss of persistent and directed migration compared with similarly treated wild-type cells
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