All Phenotypes Zap70<tm2.1Weis> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Zap70^tm2.1Weis/Zap70^tm2.1Weis	B6.129S4-Zap70^tm2.1Weis	MGI:4399127
hm2	Zap70^tm2.1Weis/Zap70^tm2.1Weis	C.129S4-Zap70^tm2.1Weis	MGI:4399130
ht3	Zap70^m1Saka/Zap70^tm2.1Weis	involves: 129S4/SvJae * BALB/c	MGI:4399132
cx4	Zap70^tm2.1Weis/Zap70^tm2.1Weis Tg(DO11.10)10Dlo/?	involves: 129S4/SvJae * BALB/c * C3H * C57BL/6	MGI:4399134
cx5	Zap70^tm2.1Weis/Zap70^tm2.1Weis Tg(TcraH-Y,TcrbH-Y)71Vbo/?	involves: 129S4/SvJae * C57BL/6J * DBA/2J	MGI:4399133

Allelic Composition	Zap70^tm2.1Weis/Zap70^tm2.1Weis
Genetic Background	B6.129S4-Zap70^tm2.1Weis

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zap70^tm2.1Weis mutation (1 available); any Zap70 mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal thymus cell ratio ( J:154166 )

• significant decrease in the relative percentage and absolute number of CD4 and CD8 single positive subsets

• however, total thymic cellularity is only mildly decreased

abnormal CD8-positive, alpha-beta T cell differentiation ( J:154166 )

• the majority of CD8+ thymocytes are at the immature intermediate stage

decreased CD4-positive, alpha-beta T cell number ( J:154166 )

• the relative percentages and absolute numbers of the CD4 single positive subsets in the thymus as well as in the periphery are substantially decreased

decreased CD8-positive, alpha-beta T cell number ( J:154166 )

• the relative percentages and absolute numbers of the CD8 single positive subsets in the thymus as well as in the periphery are substantially decreased

hematopoietic system

abnormal thymus cell ratio ( J:154166 )

• significant decrease in the relative percentage and absolute number of CD4 and CD8 single positive subsets

• however, total thymic cellularity is only mildly decreased

abnormal CD8-positive, alpha-beta T cell differentiation ( J:154166 )

• the majority of CD8+ thymocytes are at the immature intermediate stage

decreased CD4-positive, alpha-beta T cell number ( J:154166 )

• the relative percentages and absolute numbers of the CD4 single positive subsets in the thymus as well as in the periphery are substantially decreased

decreased CD8-positive, alpha-beta T cell number ( J:154166 )

• the relative percentages and absolute numbers of the CD8 single positive subsets in the thymus as well as in the periphery are substantially decreased

endocrine/exocrine glands

abnormal thymus cell ratio ( J:154166 )

• significant decrease in the relative percentage and absolute number of CD4 and CD8 single positive subsets

• however, total thymic cellularity is only mildly decreased

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:154166 )

N	• despite defects in T cell selection, mice do not display signs of autoimmunity and do not develop substantial amounts of anti-double-stranded DNA (dsDNA) autoantibodies after zymosan stimulation.

decreased T cell proliferation ( J:154166 )

• poor proliferative responses to TCR and CD28 stimulation; however, the T cells partially overcome the proliferative defect in response to higher doses

abnormal thymus cell ratio ( J:154166 )

• significant decrease in the relative percentage and absolute number of CD4 and CD8 single positive subsets

• however, total thymic cellularity is only mildly decreased

abnormal thymocyte activation ( J:154166 )

• an attenuated TCR-induced increase in cytoplasmic free calcium in thymocytes and peripheral T cells

abnormal T cell clonal deletion ( J:154166 )

• defective clonal deletion of Vbeta5 and Vbeta11 thymocytes from endogenous viral superantigens, Mtv-8 and Mtv-9

abnormal positive T cell selection ( J:154166 )

• numbers of the HSA^lo TCRbeta^hi cells are substantially decreased, as compared with thymocytes from wild-type mice

decreased CD4-positive, alpha-beta T cell number ( J:154166 )

• absolute numbers of CD4 single positive thymocytes are profoundly reduced, and fewer mature CD4+ T cells are present in the periphery

decreased CD8-positive, alpha-beta T cell number ( J:154166 )

• absolute numbers of CD8 single positive thymocytes are profoundly reduced, and fewer mature CD8+ T cells are present in the periphery

increased memory T cell number ( J:154166 )

• increase in percentages of memory CD4 + T cells

decreased regulatory T cell number ( J:154166 )

• reduction in both thymic regulatory T-cells cell frequency as well as absolute regulatory T-cell numbers

increased activated T cell number ( J:154166 )

• increase in percentage of CD45RB^lo CD4+ T cells

abnormal splenic cell ratio ( J:154166 )

• significant decrease in the absolute number of CD4 and CD8 single positive subsets

abnormal regulatory T cell physiology ( J:154166 )

• regulatory T-cells are much less efficient at suppressing proliferation of wild-type naive T cells

abnormal lymph node cell ratio ( J:154166 )

• significant decrease in the absolute number of CD4 and CD8 single positive subsets

increased interleukin-17 secretion ( J:154166 )

• the percentages of IL-17 producing cells are 6- fold higher than in the wild-type controls

decreased interleukin-2 secretion ( J:154166 )

• IL-2 production after CD3 stimulation is greatly reduced

hematopoietic system

decreased T cell proliferation ( J:154166 )

• poor proliferative responses to TCR and CD28 stimulation; however, the T cells partially overcome the proliferative defect in response to higher doses

abnormal thymus cell ratio ( J:154166 )

• significant decrease in the relative percentage and absolute number of CD4 and CD8 single positive subsets

• however, total thymic cellularity is only mildly decreased

abnormal thymocyte activation ( J:154166 )

• an attenuated TCR-induced increase in cytoplasmic free calcium in thymocytes and peripheral T cells

abnormal T cell clonal deletion ( J:154166 )

• defective clonal deletion of Vbeta5 and Vbeta11 thymocytes from endogenous viral superantigens, Mtv-8 and Mtv-9

abnormal positive T cell selection ( J:154166 )

• numbers of the HSA^lo TCRbeta^hi cells are substantially decreased, as compared with thymocytes from wild-type mice

decreased CD4-positive, alpha-beta T cell number ( J:154166 )

• absolute numbers of CD4 single positive thymocytes are profoundly reduced, and fewer mature CD4+ T cells are present in the periphery

decreased CD8-positive, alpha-beta T cell number ( J:154166 )

• absolute numbers of CD8 single positive thymocytes are profoundly reduced, and fewer mature CD8+ T cells are present in the periphery

increased memory T cell number ( J:154166 )

• increase in percentages of memory CD4 + T cells

decreased regulatory T cell number ( J:154166 )

• reduction in both thymic regulatory T-cells cell frequency as well as absolute regulatory T-cell numbers

increased activated T cell number ( J:154166 )

• increase in percentage of CD45RB^lo CD4+ T cells

abnormal splenic cell ratio ( J:154166 )

• significant decrease in the absolute number of CD4 and CD8 single positive subsets

abnormal regulatory T cell physiology ( J:154166 )

• regulatory T-cells are much less efficient at suppressing proliferation of wild-type naive T cells

endocrine/exocrine glands

abnormal thymus cell ratio ( J:154166 )

• significant decrease in the relative percentage and absolute number of CD4 and CD8 single positive subsets

• however, total thymic cellularity is only mildly decreased

abnormal thymocyte activation ( J:154166 )

• an attenuated TCR-induced increase in cytoplasmic free calcium in thymocytes and peripheral T cells

cellular

decreased T cell proliferation ( J:154166 )

• poor proliferative responses to TCR and CD28 stimulation; however, the T cells partially overcome the proliferative defect in response to higher doses

Allelic Composition	Zap70^m1Saka/Zap70^tm2.1Weis
Genetic Background	involves: 129S4/SvJae * BALB/c

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zap70^m1Saka mutation (1 available); any Zap70 mutation (55 available)
Zap70^tm2.1Weis mutation (1 available); any Zap70 mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal T cell clonal deletion ( J:154166 )

• defects in the effectiveness of Mtv-8 and Mtv-9 induced clonal deletion in thymocyte cells bearing Vbeta5 and Vbeta11

immune system

abnormal T cell clonal deletion ( J:154166 )

• defects in the effectiveness of Mtv-8 and Mtv-9 induced clonal deletion in thymocyte cells bearing Vbeta5 and Vbeta11

decreased susceptibility to induced arthritis ( J:154166 )

• only 1 of 6 heterozygous mice develop mild arthritis during the 12-wk observation compared to the Zap70^m1Saka homozygous mice which all have severe arthritis

skeleton

decreased susceptibility to induced arthritis ( J:154166 )

• only 1 of 6 heterozygous mice develop mild arthritis during the 12-wk observation compared to the Zap70^m1Saka homozygous mice which all have severe arthritis

Allelic Composition	Zap70^tm2.1Weis/Zap70^tm2.1Weis Tg(DO11.10)10Dlo/?
Genetic Background	involves: 129S4/SvJae * BALB/c * C3H * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(DO11.10)10Dlo mutation (7 available)
Zap70^tm2.1Weis mutation (1 available); any Zap70 mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal positive T cell selection ( J:154166 )

• a marked reduction in the proportion and absolute numbers of CD4 SP thymocytes, and lower percentages and lower absolute numbers of CD4+ T cells expressing the transgene in the spleens and lymph nodes

immune system

abnormal positive T cell selection ( J:154166 )

Allelic Composition	Zap70^tm2.1Weis/Zap70^tm2.1Weis Tg(TcraH-Y,TcrbH-Y)71Vbo/?
Genetic Background	involves: 129S4/SvJae * C57BL/6J * DBA/2J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcraH-Y,TcrbH-Y)71Vbo mutation (4 available)
Zap70^tm2.1Weis mutation (1 available); any Zap70 mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal T cell clonal deletion ( J:154166 )

• a substantial number of double positive and CD8 single positive thymocytes develop in male mutant mice unlike in transgenic mice wild type for Zap70

• three fold increase in total numbers of thymocytes in male mice with a nearly 40-fold increase in double positive thymocytes

abnormal positive T cell selection ( J:154166 )

• there were substantially fewer CD8+ HY-TCR+ thymocytes in female mice

immune system

abnormal T cell clonal deletion ( J:154166 )

• a substantial number of double positive and CD8 single positive thymocytes develop in male mutant mice unlike in transgenic mice wild type for Zap70

• three fold increase in total numbers of thymocytes in male mice with a nearly 40-fold increase in double positive thymocytes

abnormal positive T cell selection ( J:154166 )

• there were substantially fewer CD8+ HY-TCR+ thymocytes in female mice

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