Analysis Tools
mortality/aging
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• embryos die between E12.5 and E13.5, similar to published Hes1tm1Fgu knockouts
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Allele Symbol Allele Name Allele ID |
Hes1tm1(cre/ERT2)Lcm targeted mutation 1, L Charles Murtaugh MGI:4412375 |
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| Summary |
4 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• embryos die between E12.5 and E13.5, similar to published Hes1tm1Fgu knockouts
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• tamoxifen-treated mice exhibit normal pancreatic ductal morphology and pancreata mass
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• tamoxifen-treated mice exhibit a decrease in YFP+ centroacinar cells compared with control mice
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• tamoxifen-treated mice exhibit an increase in YFP+ acinar cells compared with control mice
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• tamoxifen-treated mice exhibit a rapid transformation of YFP+ centroacinar cells into acinar cells compared with control mice
• however, tamoxifen-treated mice exhibit normal centroacinar and acinar cells proliferation and apoptosis
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• tamoxifen-treated mice exhibit a decrease in YFP+ centroacinar cells compared with control mice
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• tamoxifen-treated mice exhibit an increase in YFP+ acinar cells compared with control mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• pancreatic progenitor cells (GFP +ve) form cystic structures, not narrow, branched structures, that lack endocrine or acinar marker expression with tamoxifen treatment (2 mg) at E9.5 or 11.5
• treatment at E13.5 or 15.5 blocks islet differentiation, but some exocrine differentiation occurs, with labeled cells integrating into normal acini and ducts
• treatment with 0.5 mg tamoxifen at E9.5 still results in formation of abnormal cystic tubules as seen with the higher dose
• Notch 1 activation at E13.5 results in most GFP +ve cells adopting a ductal, rather than acinar, fate; activation at E15.5 reverses the proportions with most cells taking an acinar fate
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 06/12/2024 MGI 6.13 |
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