Phenotypes associated with this allele

• Allele Symbol: Tg(Nes-cre)Wme
• Allele Name: transgene insertion, Werner Muller
• Allele ID: MGI:4412413
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pkd1^tm2Ggg/Pkd1^tm2Ggg Tg(Nes-cre)Wme/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:5430604
cn2	Gt(ROSA)26Sor^tm1(B4galt1)Clib/Gt(ROSA)26Sor^tm1(B4galt1)Clib Tg(Nes-cre)Wme/?	involves: 129S6/SvEvTac * C57BL/6 * CBA	MGI:5582815
cn3	Tcra^tm1Mass/Tcra^+ Tg(Nes-cre)Wme/0	involves: C57BL/6 * CBA	MGI:5532668

Genotype
MGI:5430604

cn1

• Allelic Composition: Pkd1^tm2Ggg/Pkd1^tm2Ggg; Tg(Nes-cre)Wme/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

renal/urinary system

increased kidney apoptosis ( J:185855 )

• cyst-lining epithelial cells are highly apoptotic

increased kidney cell proliferation ( J:185855 )

• cyst-lining epithelial cells are highly proliferative

• -morphologically normal tubules and interstitial cells exhibit increased proliferation

abnormal kidney morphology ( J:185855 )

• kidneys show extensive deposition of collagen

kidney cyst ( J:185855 )

• mutants develop renal cysts by 49 days of age

• renal cysts arise predominately from collecting duct/distal tubules

• mutants treated with rapamycin exhibit an inhibition of renal cyst growth, a decrease in plasma blood urea nitrogen, fibrosis, and proliferation of cystic cells but show an increases cystic cell apoptosis (but not in normal tubule epithelial cells)

enlarged kidney ( J:185855 )

• kidneys are enlarged in 49 day old mutants

renal fibrosis ( J:185855 )

homeostasis/metabolism

increased blood urea nitrogen level ( J:185855 )

• mutants exhibit highly elevated blood urea nitrogen

cellular

increased kidney apoptosis ( J:185855 )

• cyst-lining epithelial cells are highly apoptotic

increased kidney cell proliferation ( J:185855 )

• cyst-lining epithelial cells are highly proliferative

• -morphologically normal tubules and interstitial cells exhibit increased proliferation

growth/size/body

kidney cyst ( J:185855 )

• mutants develop renal cysts by 49 days of age

• renal cysts arise predominately from collecting duct/distal tubules

• mutants treated with rapamycin exhibit an inhibition of renal cyst growth, a decrease in plasma blood urea nitrogen, fibrosis, and proliferation of cystic cells but show an increases cystic cell apoptosis (but not in normal tubule epithelial cells)

enlarged kidney ( J:185855 )

• kidneys are enlarged in 49 day old mutants

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
polycystic kidney disease 1		DOID:0110858	OMIM:173900	J:185855

Genotype
MGI:5582815

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm1(B4galt1)Clib}/Gt(ROSA)26Sor^{tm1(B4galt1)Clib}; Tg(Nes-cre)Wme/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CBA

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:209737 )

• mice exhibit resistance to TNF-induced inflammatory shock with increased survival compared with control mice

digestive/alimentary system

digestive/alimentary phenotype ( J:209737 )

N • mice exhibit normal gut mucosa morphology

abnormal Paneth cell morphology ( J:209737 )

• reduced granule content

abnormal gut flora balance ( J:209737 )

• higher Firmicutes to Bacteroidetes ratio

decreased susceptibility to induced colitis ( J:209737 )

• DSS-treated mice exhibit less severe colitis symptoms (reduced rectal bleeding, diarrhea, colon shortening and increase in gut permeability without loss of crypt architecture or reduction in goblet cells) compared with control mice

immune system

decreased susceptibility to induced colitis ( J:209737 )

• DSS-treated mice exhibit less severe colitis symptoms (reduced rectal bleeding, diarrhea, colon shortening and increase in gut permeability without loss of crypt architecture or reduction in goblet cells) compared with control mice

decreased circulating interleukin-6 level ( J:209737 )

• in TNF-injected mice

decreased acute inflammation ( J:209737 )

• mice exhibit resistance to TNF-induced inflammatory shock with increased survival, faster recovery from initial drop in body temperature and reduced serum IL6 levels compared with control mice

• however, infiltration capacity of leukocytes is not altered

homeostasis/metabolism

abnormal homeostasis ( J:209737 )

• mice exhibit increase in galactose terminating N-glycans in ileum and colon mucus compared with control mice

decreased circulating interleukin-6 level ( J:209737 )

• in TNF-injected mice

hematopoietic system

hematopoietic system phenotype ( J:209737 )

N • mice exhibit normal blood composition

endocrine/exocrine glands

abnormal Paneth cell morphology ( J:209737 )

• reduced granule content

Genotype
MGI:5532668

cn3

• Allelic Composition: Tcra^tm1Mass/Tcra⁺; Tg(Nes-cre)Wme/0
• Genetic Background: involves: C57BL/6 * CBA

immune system

decreased thymocyte number ( J:201417 )

abnormal positive T cell selection ( J:201417 )

• perturbed

increased double-negative T cell number ( J:201417 )

decreased NK T cell number ( J:201417 )

• in the thymus and spleen

hematopoietic system

decreased thymocyte number ( J:201417 )

abnormal positive T cell selection ( J:201417 )

• perturbed

increased double-negative T cell number ( J:201417 )

decreased NK T cell number ( J:201417 )

• in the thymus and spleen

endocrine/exocrine glands

decreased thymocyte number ( J:201417 )