Analysis Tools
muscle
| N |
• gastrocnemius and soleus muscle mass are not different from wild type
• no differences in muscle fiber morphology or in number of centrally located myofiber nuclei in gastrocnemius muscle compared to wild type
|
|
• lipid oxidative damage is elevated in TA muscles of young mice
|
|
• mitochondria isolated from glycolytic skeletal muscle of young mice release greater than two-fold more superoxide than those from glycolytic muscle of wild type controls
|
|
• lactate levels differ from wild type significantly after 40 minutes of running
|
|
• blood glucose levels are not significantly different up to 40 minutes of running but mutant mice utilize glucose at a fourfold faster rate than wild type from 40 minutes of running until exhaustion
|
|
• glycolytic muscle function and running capacity are reduced in young mice compared to wild type
• maximum contractile force production in glycolytic and oxidative muscle is not different from wild type, while a significant decline in force during repeated contractions of the extensor digitorum longus or gastrocnemius is observed beyond that measured in wild type mice
• average minimum force produced by gastrocnemius muscle is 10% less than that in wild type, but that produced by the soleus muscle is not different from wild type
|
growth/size/body
| N |
• body mass in young female (5- to 8-month old) and male (3- to 4-month old) mice are not different from wild type; lean body mass is not different from wild type
|
cellular
|
• mitochondria isolated from glycolytic skeletal muscle of young mice release greater than two-fold more superoxide than those from glycolytic muscle of wild type controls
|
|
• blood glucose levels are not significantly different up to 40 minutes of running but mutant mice utilize glucose at a fourfold faster rate than wild type from 40 minutes of running until exhaustion
|
|
• rate of peroxide (H2O2) production is reduced by about 33% with complex II-linked substrate (succinate+rotenone) but increased (by 56%) in the presence of a complex III inhibitor
|
adipose tissue
| N |
• no differences in body fat are detected compared with wild type
|
homeostasis/metabolism
| N |
• blood lactate and glucose fasting levels and nonfasted levels before exercise are not different from wild type
|
|
• distance run on the treadmill is 55% less than the distance run by wild type
|
|
• average time of exhaustion in treadmill running is about 63 minutes compared to about 139 minutes in wild type animals
|
|
• the manganese-superoxide dismutase (Mn-SOD) intensity/fiber type area in type IIB muscle fibers is reduced by 70% relative to wild type
• aconitase protein in homogenates of glycolytic skeletal muscle from young female mice is reduced by 50%, whereas levels in soleus muscle is not different from controls
|
|
• aconitase activity in homogenates of glycolytic skeletal muscle is reduced by 56% and 52% in young male and female mice compared to wild type, whereas levels in soleus muscle is not different from controls
|
behavior/neurological
|
• distance run on the treadmill is 55% less than the distance run by wild type
|
|
• average time of exhaustion in treadmill running is about 63 minutes compared to about 139 minutes in wild type animals
|
limbs/digits/tail
