Phenotypes associated with this allele

• Allele Symbol: Gad2^tm2(cre)Zjh
• Allele Name: targeted mutation 2, Z Josh Huang
• Allele ID: MGI:4418713
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gad2^tm2(cre)Zjh/Gad2^tm2(cre)Zjh	involves: 129S4/SvJae * C57BL/6 * SJL	MGI:4418723
cn2	Gad2^tm2(cre)Zjh/? Rai1^tm2.1Luo/Rai1^tm2.1Luo	either: (involves: 129S1/Sv * 129S4/SvJae * C57BL/6) or (involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6)	MGI:5817645
cn3	Gigyf1^em1Xio/Gigyf1^em1Xio Gad2^tm2(cre)Zjh/Gad2^+	involves: 129S4/SvJae * C57BL/6	MGI:7779934
cn4	Gabrd^tm1.1Jmag/Gabrd^+ Gad2^tm2(cre)Zjh/Gad2^+	involves: 129S4/SvJae * C57BL/6 * SJL	MGI:5527106

Genotype
MGI:4418723

hm1

• Allelic Composition: Gad2^tm2(cre)Zjh/Gad2^tm2(cre)Zjh
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:151755 )

• mice are viable, fertile, normal in size and display no gross physical or behavioral abnormalities

Genotype
MGI:5817645

cn2

• Allelic Composition: Gad2^tm2(cre)Zjh/?; Rai1^tm2.1Luo/Rai1^tm2.1Luo
• Genetic Background: either: (involves: 129S1/Sv * 129S4/SvJae * C57BL/6) or (involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6)

behavior/neurological

abnormal associative learning ( J:237687 )

♂

impaired contextual conditioning behavior ( J:237687 )

♂ • decreased freezing behavior in contextual memory recall

impaired cued conditioning behavior ( J:237687 )

♂ • decreased freezing behavior in cued recall

Genotype
MGI:7779934

cn3

• Allelic Composition: Gigyf1^em1Xio/Gigyf1^em1Xio; Gad2^tm2(cre)Zjh/Gad2⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6

behavior/neurological

abnormal object recognition memory ( J:335507 )

♂ • mice exhibit an impairment in cognitive performance in the novel object recognition test

♂ • however, mice do not show changes in sociability, social novelty, rearing, marble burying, elevated plus maze, or T-maze

increased thigmotaxis ( J:335507 )

♂ • mice exhibit elevated anxiety levels, spending less time in centers of the open field

decreased grooming behavior ( J:335507 )

♂ • mice exhibit reduced grooming behavior

Genotype
MGI:5527106

cn4

• Allelic Composition: Gabrd^tm1.1Jmag/Gabrd⁺; Gad2^tm2(cre)Zjh/Gad2⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * SJL

nervous system

decreased susceptibility to pharmacologically induced seizures ( J:203336 )

• decreased seizure susceptibility in response 20mg/kg kainic acid as compared to controls

• decreased epilepitform activity over 120 min recording period

impaired ability to fire action potentials ( J:203336 )

• decreased average number of action potentials fired in CA1 pyramidal neurons in response to current injections above 40 pA

• decreased average number of action potentials fired in dentate gyrus granule cells in response to current injections above 60 pA

abnormal GABAergic neuron physiology ( J:203336 )

• decrease in tonic GABAergic currents recorded in interneurons from the molecular layer of the hippocampus as compared to controls

• decrease in tonic GABAergic currents recorded in interneurons from the stratum radiatum layer of the hippocampus as compared to controls

• increase in tonic GABAergic currents recorded in CA1 pyramidal neurons, however, no difference is observed in dentate gyrus granule cells as compared to controls

abnormal CNS synaptic transmission ( J:203336 )

• increase in spontaneous firing rate of stratum radiatum layer interneurons as compared to controls

• increase in firing rate of stratum radiatum layer interneurons in response to a single 1 s, 100 pA current injection as compared to controls

• increase in firing rate of molecular layer interneurons in response to a single 1 s, 100 pA current injection as compared to controls

• no differences are observed in resting potential or input resistance

increased excitatory postsynaptic current amplitude ( J:203336 )

• half maximal current amplitude (I₅₀) is increased in CA1 pyramidal neurons and dentate gyrus granule cells as compared to controls

abnormal inhibitory postsynaptic currents ( J:203336 )

• frequency of spontaneous IPSCs is increased in dentate gyrus granule cells (DGGCs) and CA1 pyramidal neurons

• peak amplitude of spontaneous IPSCs is increased in DGGCs and CA1 pyramidal neurons

• decrease in average inter-event interval (IEI) of spontaneous IPSCs

• increase in percentage of DGGCs and CA1 pyramidal cells following bursts of inhibitory postsynaptic currents as compared to control

behavior/neurological

decreased susceptibility to pharmacologically induced seizures ( J:203336 )

• decreased seizure susceptibility in response 20mg/kg kainic acid as compared to controls

• decreased epilepitform activity over 120 min recording period