Phenotypes associated with this allele

• Allele Symbol: Tg(tetO-LRRK2)C7874Cai
• Allele Name: transgene insertion C7874, Huaibin Cai
• Allele ID: MGI:4420758
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(Camk2a-tTA)1Mmay/0 Tg(tetO-LRRK2)C7874Cai/0	involves: C57BL/6	MGI:4421001
cx2	Tg(Camk2a-tTA)1Mmay/0 Tg(tetO-LRRK2)C7874Cai/0 Tg(tetO-SNCA*A53T)E2Cai/0	involves: C57BL/6	MGI:4421002

Genotype
MGI:4421001

cx1

• Allelic Composition: Tg(Camk2a-tTA)1Mmay/0; Tg(tetO-LRRK2)C7874Cai/0
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

nervous system phenotype ( J:156512 )

N • no gross neuropathological phenotypes are observed in 12-month old animals

N • no significant changes in neuron counts are observed compared to non-transgenic controls at the frontal cortex or dorsal striatum in 6- or 20-month old mice

abnormal neuron morphology ( J:156512 )

• the Golgi complex is drastically altered in neurons at 1 month, appearing thinner and fragmented

• at 6 months fragmentation of the cis-Golgi apparatus is similar to that in LRRK2WT and A53T/LRRK2 neurons

• at 1 month, the medial/trans-Golgi in neurons is significantly altered with ratio of fragmented trans-Golgi significantly increased compared to non-transgenic animals

Genotype
MGI:4421002

cx2

• Allelic Composition: Tg(Camk2a-tTA)1Mmay/0; Tg(tetO-LRRK2)C7874Cai/0; Tg(tetO-SNCA*A53T)E2Cai/0
• Genetic Background: involves: C57BL/6

nervous system

abnormal nervous system morphology ( J:156512 )

• increasing accumulation of alpha-synuclein is detected in cell bodies at 1 month compared to A53T/LRRK2WT-L animals

microgliosis ( J:156512 )

• increased microglial activation is observed in the brain

abnormal dorsal striatum morphology ( J:156512 )

• neuron loss is detected at 6 months and later

abnormal cerebral cortex morphology ( J:156512 )

astrocytosis ( J:156512 )

• A53T/LRRK2WT mice show significant elevation of GFAP-positive astrocytes in the striatum compared to A53T mice

• more GFAP-positive astrocytes are found in the brain than in A53T/LRRK2WT-L brains

abnormal neuron morphology ( J:156512 )

• the Golgi complex is drastically altered in neurons at 1 month with severely fragmented cis-Golgi

• at 1 month, the medial/trans-Golgi in neurons is severely fragmented

neurodegeneration ( J:156512 )

• neurodegeneration is accelerated in the striatum

• dramatic loss (>80%) of striatal neurons is seen in the dorsal striatum

• most degenerating neurons are striatal medium-sized spiny neurons (MSN)

abnormal nervous system physiology ( J:156512 )

• neuropathology is accelerated in mice compared to A53T mice

• in 6 month old mice, elevated levels of ubiquitin are detected in somas and nuclei of cortical neurons; levels of alpha-synuclein and ubiquitin are slightly higher than in A53T mutant mice

• brain homogenates contain significantly elevated levels of ubiquitinated proteins at 3 months

immune system

microgliosis ( J:156512 )

• increased microglial activation is observed in the brain

hematopoietic system

microgliosis ( J:156512 )

• increased microglial activation is observed in the brain