Phenotypes associated with this allele

• Allele Symbol: Mcph1^tm1.2Kali
• Allele Name: targeted mutation 1.2, Kaiyi Li
• Allele ID: MGI:4429492
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mcph1^tm1.2Kali/Mcph1^tm1.2Kali	involves: 129S7/SvEvBrd * C57BL/6J	MGI:4429495

Genotype
MGI:4429495

hm1

• Allelic Composition: Mcph1^tm1.2Kali/Mcph1^tm1.2Kali
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Mcph1^tm1.2Kali/Mcph1^tm1.2Kali males are infertile and exhibit meiotic defects

mortality/aging

increased mortality induced by gamma-irradiation ( J:156371 )

• when exposed to 7 Gy ionizing radiation, all mice die within 9 days, where 80% of wild-type mice survive

perinatal lethality, incomplete penetrance ( J:156371 )

• birth rate is lower than expected, no time of lethality provided

reproductive system

abnormal spermatid morphology ( J:156371 )

♂ • at P56, absence of spermatids in seminiferous tubules

decreased male germ cell number ( J:156371 )

♂ • fewer spermatocytes are produced in seminiferous tubules

abnormal male meiosis ( J:156371 )

♂ • interstitial initiation of synapsis with asynapsis on either side of the contact

♂ • incomplete, dashed-line shape bivalents in many homologs during zygotene or zygotene/pachytene

♂ • double-strand breaks cannot be appropriately repaired

arrest of male meiosis ( J:156371 )

♂ • meiotic dysregulation at P14, P21, and P56, spermatocytes arrest prior to the pachytene stage

♂ • increase in zygotene spermatocytes and a complete absence of postmeitotic cells

increased male germ cell apoptosis ( J:156371 )

♂ • increase in TUNEL+ cells in tubules

absent ovarian follicles ( J:156371 )

♀

decreased ovary weight ( J:156371 )

♀

abnormal seminiferous tubule morphology ( J:156371 )

♂ • significantly smaller and thinner than those of wild-type at P28

small testis ( J:156371 )

♂ • testes are consistently smaller especially after 3-weeks of age

female infertility ( J:156371 )

♀

male infertility ( J:156371 )

♂

endocrine/exocrine glands

absent ovarian follicles ( J:156371 )

♀

decreased ovary weight ( J:156371 )

♀

abnormal seminiferous tubule morphology ( J:156371 )

♂ • significantly smaller and thinner than those of wild-type at P28

small testis ( J:156371 )

♂ • testes are consistently smaller especially after 3-weeks of age

cellular

abnormal spermatid morphology ( J:156371 )

♂ • at P56, absence of spermatids in seminiferous tubules

decreased male germ cell number ( J:156371 )

♂ • fewer spermatocytes are produced in seminiferous tubules

abnormal male meiosis ( J:156371 )

♂ • interstitial initiation of synapsis with asynapsis on either side of the contact

♂ • incomplete, dashed-line shape bivalents in many homologs during zygotene or zygotene/pachytene

♂ • double-strand breaks cannot be appropriately repaired

arrest of male meiosis ( J:156371 )

♂ • meiotic dysregulation at P14, P21, and P56, spermatocytes arrest prior to the pachytene stage

♂ • increase in zygotene spermatocytes and a complete absence of postmeitotic cells

increased cellular sensitivity to gamma-irradiation ( J:156371 )

• MEFs are more sensitive to ionizing radiation, as measure by number of cells surviving after 6 days

increased male germ cell apoptosis ( J:156371 )

♂ • increase in TUNEL+ cells in tubules

induced chromosome breakage ( J:156371 )

• increased chromosomal breakage in MEFs 3 and 20 hrs after ionizing radiation treatment

growth/size/body

decreased body weight ( J:156371 )

• at P56 weight is only 80% of wild-type

immune system

abnormal T cell physiology ( J:156371 )

• increase in number of chromosomal aberrations in T cells

hematopoietic system

abnormal T cell physiology ( J:156371 )

• increase in number of chromosomal aberrations in T cells

homeostasis/metabolism

increased mortality induced by gamma-irradiation ( J:156371 )

• when exposed to 7 Gy ionizing radiation, all mice die within 9 days, where 80% of wild-type mice survive