Phenotypes associated with this allele

• Allele Symbol: Cux2^tm1.1Nieto
• Allele Name: targeted mutation 1.1, Marta Nieto
• Allele ID: MGI:4429541
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cux2^tm1.1Nieto/Cux2^tm1.1Nieto	involves: 129S4/SvJae * C57BL/6 * FVB/N * Swiss Webster	MGI:4429542
cx2	Cux1^tm2Ejn/Cux1^tm2Ejn Cux2^tm1.1Nieto/Cux2^tm1.1Nieto	involves: 129S4/SvJae * C57BL/6 * FVB/N * Swiss Webster	MGI:4429544

Genotype
MGI:4429542

hm1

• Allelic Composition: Cux2^tm1.1Nieto/Cux2^tm1.1Nieto
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * FVB/N * Swiss Webster

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal neuronal precursor proliferation ( J:156578 )

• increase in proliferation of neuronal precursors as measured by increased BrdU positive cells and diluted staining.

• an increase in number of double labeled cells at P21 when injected at E14.5 with IdU and 10 hours later with CldU indicating an increased proportion of neurons have been generated from precursors that reentered the cell cycle

increased brain size ( J:156578 )

• brains are moderately but consistently bigger in volume

abnormal cerebral cortex morphology ( J:156578 )

abnormal neocortex morphology ( J:156578 )

• both cell density and the thickness of upper cortical layers (II--IV) are greater

• selective increase in the number of upper layer neurons

thickened cerebral cortex ( J:156578 )

• moderate increase in the thickness of upper layers

abnormal embryonic/fetal subventricular zone morphology ( J:156578 )

• at E15.5 the number of Ki67+ or BrdU+ cells is dramatically increased

• the number of cells in basal mitosis is greatly increased in the subventricular zone at E15.5

• the number of intermediate neuronal progenitors in the proliferative regions at E15.5 is dramatically increased

increased neuron number ( J:156578 )

• an increase in the total number of upper layer neurons at E13.5, E14.5, and E16.5

cellular

abnormal neuronal precursor proliferation ( J:156578 )

• increase in proliferation of neuronal precursors as measured by increased BrdU positive cells and diluted staining.

• an increase in number of double labeled cells at P21 when injected at E14.5 with IdU and 10 hours later with CldU indicating an increased proportion of neurons have been generated from precursors that reentered the cell cycle

Genotype
MGI:4429544

cx2

• Allelic Composition: Cux1^tm2Ejn/Cux1^tm2Ejn; Cux2^tm1.1Nieto/Cux2^tm1.1Nieto
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * FVB/N * Swiss Webster

mortality/aging

lethality throughout fetal growth and development, incomplete penetrance ( J:156578 )

• most die between E9 and E15; some mice do develop to birth

nervous system

nervous system phenotype ( J:156578 )

N • in the small number that survive to P0, the cortex appears to be normally laminated

increased neuron number ( J:156578 )

• the number of E16.5-birthdated neurons is significantly increased but not different than Cux2^tm1.1Nieto