Phenotypes associated with this allele

• Allele Symbol: Fgf8^tm1.1Jyhl
• Allele Name: targeted mutation 1.1, James Y H Li
• Allele ID: MGI:4430251
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fgf8^tm1.1Jyhl/Fgf8^tm1.1Jyhl	involves: CD-1	MGI:4430255
cn2	En1^tm7(cre/ESR1)Alj/0 Fgf8^tm1.1Jyhl/Fgf8^tm1.3Mrt	involves: 129P2/OlaHsd * 129S6/SvEvTac	MGI:4437222
cx3	Fgf17^tm1Dor/Fgf17^tm1Dor Fgf8^tm1.1Jyhl/Fgf8^tm1.1Jyhl	involves: 129S6/SvEvTac	MGI:4437230

Genotype
MGI:4430255

hm1

• Allelic Composition: Fgf8^tm1.1Jyhl/Fgf8^tm1.1Jyhl
• Genetic Background: involves: CD-1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

abnormal pharyngeal arch morphology ( J:135128 )

• malformed branchial arches at E9.5

abnormal developmental patterning ( J:135128 )

• the anteroposterior (AP) axis aligns with the short, rather than the long, transverse axis of the embryo at E6.5

• the AP axis of the embryo is parallel with the long uterine axis at E6.5

• normal long axis of the embryo in register with the long uterine axis at E6.5

abnormal mesoderm development ( J:135128 )

• abnormal embryonic mesoderm derived structures at E8.5 in 21.6% of the mutant (11/51)

• normal somites and cardiomesoderm at E8.5 in 78.4% of the mutant (40/51)

decreased embryo size ( J:135128 )

• smaller in size than wild-type at E9.5

open neural tube ( J:135128 )

• at E9.5

abnormal primitive streak morphology ( J:135128 )

• accumulation of mesodermal cells at the primitive streak at E7.5

absent primitive node ( J:135128 )

• lack of morphologically distinct node structure at E7.5

abnormal amnion morphology ( J:135128 )

• the amniotic membrane appears rough and fluffy

growth/size/body

decreased embryo size ( J:135128 )

• smaller in size than wild-type at E9.5

nervous system

open neural tube ( J:135128 )

• at E9.5

craniofacial

abnormal pharyngeal arch morphology ( J:135128 )

• malformed branchial arches at E9.5

cardiovascular system

abnormal heart tube morphology ( J:135128 )

• malformed heart tubes at E9.5

Genotype
MGI:4437222

cn2

• Allelic Composition: En1^{tm7(cre/ESR1)Alj}/0; Fgf8^tm1.1Jyhl/Fgf8^tm1.3Mrt
• Genetic Background: involves: 129P2/OlaHsd * 129S6/SvEvTac

mortality/aging

perinatal lethality, complete penetrance ( J:156717 )

• die at birth

nervous system

abnormal neural tube morphology ( J:156717 )

• a significant increase in the number of dead cells in the mesencephalon and to a less extent in rhombomere of the neural tube of embryos at the 13-somite stage

abnormal rhombomere morphology ( J:156717 )

• a significant increase in the number of dead cells in rhombomere of the neural tube of embryos at the 13-somite stage

abnormal Raphe nucleus morphology ( J:156717 )

• a reduction of serotonergic neurons in raphe nuclei

abnormal midbrain morphology ( J:156717 )

• E18.5 revealed a nearly complete loss of the midbrain and the cerebellum

• a significant increase in the number of dead cells in the mesencephalon at the 13-somite stage

abnormal substantia nigra morphology ( J:156717 )

• depletion of dopaminergic neurons at E18.5

small cerebellum ( J:156717 )

• E18.5 revealed a nearly complete loss of the midbrain and the cerebellum

decreased dopaminergic neuron number ( J:156717 )

• at E18.5 in the substantia nigra and ventral tegmental area

abnormal serotonergic neuron morphology ( J:156717 )

• at E18.5 a reduction of serotonergic neurons in the raphe nucleus

embryo

abnormal neural tube morphology ( J:156717 )

• a significant increase in the number of dead cells in the mesencephalon and to a less extent in rhombomere of the neural tube of embryos at the 13-somite stage

abnormal rhombomere morphology ( J:156717 )

• a significant increase in the number of dead cells in rhombomere of the neural tube of embryos at the 13-somite stage

Genotype
MGI:4437230

cx3

• Allelic Composition: Fgf17^tm1Dor/Fgf17^tm1Dor; Fgf8^tm1.1Jyhl/Fgf8^tm1.1Jyhl
• Genetic Background: involves: 129S6/SvEvTac

nervous system

decreased inferior colliculus size ( J:156717 )

• the tissue between the inferior colliculus and the anterior cerebellum is significantly reduced in adult mice

abnormal cerebellum anterior vermis morphology ( J:156717 )

• lobules I-III are truncated and considerably smaller in size in adult mice