Phenotypes associated with this allele

• Allele Symbol: Epha4^tm1.2Bzh
• Allele Name: targeted mutation 1.2, Binhai Zheng
• Allele ID: MGI:4430286
• Summary: 9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Epha4^tm1.2Bzh/Epha4^tm1.2Bzh	involves: 129S7/SvEvBrd	MGI:5614428
hm2	Epha4^tm1.2Bzh/Epha4^tm1.2Bzh	involves: 129S7/SvEvBrd * C57BL/6 * FVB/N	MGI:4430290
cn3	Epha4^tm1.1Bzh/Epha4^tm1.2Bzh Emx1^tm1(cre)Krj/Emx1^+	involves: 129S2/SvPas * 129S7/SvEvBrd	MGI:5614429
cn4	Epha4^tm1.1Bzh/Epha4^tm1.2Bzh Tg(Slc17a6-icre)1Oki/0	involves: 129S7/SvEvBrd * C57BL/6	MGI:5614433
cn5	Epha4^tm1.1Bzh/Epha4^tm1.2Bzh Tg(Hoxb8-cre)1403Uze/0	involves: 129S7/SvEvBrd * C57BL/6 * DBA/2	MGI:5614430
cx6	Epha4^tm1.2Bzh/Epha4^tm1.2Bzh Tg(Hlxb9-GFP)1Tmj/0	involves: 129S/Sv * C57BL/6J * CD-1 * FVB/N	MGI:6406414
cx7	Chn1^tm1.2Ece/Chn1^tm1.2Ece Epha4^tm1.2Bzh/Epha4^tm1.2Bzh Tg(Hlxb9-GFP)1Tmj/0	involves: 129S/Sv * C57BL/6J * CD-1 * FVB/N	MGI:6406415
cx8	Chn1^tm1.2Ece/Chn1^+ Epha4^tm1.2Bzh/Epha4^tm1.2Bzh Tg(Hlxb9-GFP)1Tmj/0	involves: 129S/Sv * C57BL/6J * CD-1 * FVB/N	MGI:6406417
cx9	Chn1^tm1.1Ece/Chn1^tm1.1Ece Epha4^tm1.2Bzh/Epha4^tm1.2Bzh Tg(Hlxb9-GFP)1Tmj/0	involves: 129S/Sv * C57BL/6J * CD-1 * FVB/N	MGI:6406444

Genotype
MGI:5614428

hm1

• Allelic Composition: Epha4^tm1.2Bzh/Epha4^tm1.2Bzh
• Genetic Background: involves: 129S7/SvEvBrd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal gait ( J:209606 )

• quadrupedal hopping gait is dominant at all locomotor frequencies

• occasional alternating gait is seen at lower frequencies

nervous system

abnormal spinal cord morphology ( J:209606 )

• significantly wider gap between the central canal and the ventral edge of the dorsal funiculus

abnormal spinal cord dorsal column morphology ( J:209606 )

• wider and the distance from the ventral edge to the surface of the spinal cord is shorter; however, the total area is not different from controls

Genotype
MGI:4430290

hm2

• Allelic Composition: Epha4^tm1.2Bzh/Epha4^tm1.2Bzh
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * FVB/N

behavior/neurological

abnormal gait ( J:157159 )

• hindlimb hopping gait

Genotype
MGI:5614429

cn3

• Allelic Composition: Epha4^tm1.1Bzh/Epha4^tm1.2Bzh; Emx1^tm1(cre)Krj/Emx1⁺
• Genetic Background: involves: 129S2/SvPas * 129S7/SvEvBrd

behavior/neurological

behavior/neurological phenotype ( J:209606 )

N • unlike in germ line null mice, the gait is similar to that in wild-type mice

nervous system

abnormal corticospinal tract morphology ( J:209606 )

• increased midline re-crossing of axons dorsal to the central canal in both the cervical enlargement and lumbar spinal cord

Genotype
MGI:5614433

cn4

• Allelic Composition: Epha4^tm1.1Bzh/Epha4^tm1.2Bzh; Tg(Slc17a6-icre)1Oki/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

behavior/neurological

abnormal gait ( J:209606 )

• quadrupedal hopping gait is dominant at all locomotor frequencies

• occasional alternating gait is seen at lower frequencies

nervous system

abnormal corticospinal tract morphology ( J:209606 )

• increase in axon midline crossing

abnormal spinal cord morphology ( J:209606 )

• significantly wider gap between the central canal and the ventral edge of the dorsal funiculus

abnormal spinal cord dorsal column morphology ( J:209606 )

• wider and the distance from the ventral edge to the surface of the spinal cord is shorter; however, the total area is not different from controls

abnormal nerve fiber response ( J:209606 )

• chemically and electrically stimulated locomotor-like activity in isolated spinal cord preparations induces a synchronous "hopping" locomotor-like activity rather than an alternating locomotor-like activity

Genotype
MGI:5614430

cn5

• Allelic Composition: Epha4^tm1.1Bzh/Epha4^tm1.2Bzh; Tg(Hoxb8-cre)1403Uze/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * DBA/2

behavior/neurological

abnormal gait ( J:209606 )

• dominant hopping hindlimb gait at all frequencies of locomotion with occasional alternating gait at frequencies around and less than 4 Hz

nervous system

abnormal spinal cord morphology ( J:209606 )

• significantly wider gap between the central canal and the ventral edge of the dorsal funiculus

abnormal spinal cord dorsal column morphology ( J:209606 )

• wider and the distance from the ventral edge to the surface of the spinal cord is shorter; however, the total area is not different from controls

Genotype
MGI:6406414

cx6

• Allelic Composition: Epha4^tm1.2Bzh/Epha4^tm1.2Bzh; Tg(Hlxb9-GFP)1Tmj/0
• Genetic Background: involves: 129S/Sv * C57BL/6J * CD-1 * FVB/N

nervous system

abnormal axon fasciculation ( J:243785 )

• abducens nerves appear less defasciculated than in Chn1^tm1.2Ece homozygotes

abnormal innervation ( J:243785 )

• aberrant abducens nerve fascicles wander more than in Chn1^tm1.2Ece homozygotes and some aberrant abducens nerve fascicles track with the mandibular and cervical branches of the facial nerve, rather than the buccal branch

• embryos show minor aberrant innervation of the lateral rectus from the oculomotor nerve

• however, trochlear nerve and first spine projections are unaffected

decreased motor neuron number ( J:243785 )

• E13.5 mutants have approximately half the number of abducens motor neurons than in wild-type

abnormal abducens nerve morphology ( J:243785 )

• in orbits, the lateral rectus muscle is appropriately innervated by the abducens nerve, although occasionally the nerve appears thinner

cellular

abnormal axon fasciculation ( J:243785 )

• abducens nerves appear less defasciculated than in Chn1^tm1.2Ece homozygotes

Genotype
MGI:6406415

cx7

• Allelic Composition: Chn1^tm1.2Ece/Chn1^tm1.2Ece; Epha4^tm1.2Bzh/Epha4^tm1.2Bzh; Tg(Hlxb9-GFP)1Tmj/0
• Genetic Background: involves: 129S/Sv * C57BL/6J * CD-1 * FVB/N

nervous system

abnormal innervation ( J:243785 )

• 2 of 5 embryos show orbital innervation failure by abducens nerve

• abducens nerve shows enhanced wandering of abducens bundles

• however, embryos show normalized abducens exit and fasciculation

• embryos show increased abducens nerve stalling compared to homozygous Chn1^tm1.1Ece homozygotes and similar abducens nerve wandering compared to Epha4^tm1.2Bzh homozygotes

• stalled projections do not stay within normal nerve boundaries and instead wander to track with the mandibular and cervical branches of the facial nerve

• the abducens nerve fails to innervate the lateral rectus at E16.5, which instead is innervated by aberrant branches of the oculomotor nerve; this phenotype is worse than seen in single Epha4^tm1.2Bzh homozygotes

abnormal abducens nerve morphology ( J:243785 )

• embryos show a reduction in abducens nerve length and diameter at the orbit

Genotype
MGI:6406417

cx8

• Allelic Composition: Chn1^tm1.2Ece/Chn1⁺; Epha4^tm1.2Bzh/Epha4^tm1.2Bzh; Tg(Hlxb9-GFP)1Tmj/0
• Genetic Background: involves: 129S/Sv * C57BL/6J * CD-1 * FVB/N

nervous system

abnormal innervation ( J:243785 )

• while embryos show normalized abducens exit and fasciculation, abducens nerve stalling is increased in the abducens fasciculation region, with both reduced abducens nerve length and thinner nerve diameter near the orbit

• stalled projections do not stay within normal nerve boundaries and instead wander to track with the mandibular and cervical branches of the facial nerve

abnormal abducens nerve morphology ( J:243785 )

• abducens nerve length is reduced in embryos and nerve diameter is thinner near the orbit

Genotype
MGI:6406444

cx9

• Allelic Composition: Chn1^tm1.1Ece/Chn1^tm1.1Ece; Epha4^tm1.2Bzh/Epha4^tm1.2Bzh; Tg(Hlxb9-GFP)1Tmj/0
• Genetic Background: involves: 129S/Sv * C57BL/6J * CD-1 * FVB/N

nervous system

abnormal innervation ( J:243785 )

• embryos exhibit trochlear nerve branching abnormalities similar to those of Chn1^tm1.1Ece homozygous embryos

• however, the first cervical spine projections are normal