Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^{tm8(Map2k1*,EGFP)Rsky}
• Allele Name: targeted mutation 8, Klaus Rajewsky
• Allele ID: MGI:4430550
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Egr2^tm2(cre)Pch/Egr2^+ Gt(ROSA)26Sor^tm8(Map2k1*,EGFP)Rsky/? Ptpn11^tm1.1Wbm/Ptpn11^tm1.1Wbm	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6	MGI:5588145
cn2	Gt(ROSA)26Sor^tm8(Map2k1*,EGFP)Rsky/Gt(ROSA)26Sor^tm8(Map2k1*,EGFP)Rsky Igh-J^tm1Cgn/Igh^tm4Cgn Tg(Cr2-cre)3Cgn/0	involves: 129P2/OlaHsd * C57BL/6	MGI:4430561
cn3	Erbb3^tm3Cbm/Erbb3^tm3Cbm Gt(ROSA)26Sor^tm8(Map2k1*,EGFP)Rsky/? Tg(Dhh-cre)1Mejr/0	involves: 129P2/OlaHsd * C57BL/6 * FVB/N	MGI:5588163
cn4	Gt(ROSA)26Sor^tm8(Map2k1*,EGFP)Rsky/? Ptpn11^tm1.1Wbm/Ptpn11^tm1.1Wbm Tg(Dhh-cre)1Mejr/0	involves: 129P2/OlaHsd * C57BL/6 * FVB/N	MGI:5588150
cn5	Egr2^tm2(cre)Pch/Egr2^+ Gt(ROSA)26Sor^tm8(Map2k1*,EGFP)Rsky/?	involves: 129S2/SvPas * C57BL/6	MGI:5588113
cn6	Gt(ROSA)26Sor^tm8(Map2k1*,EGFP)Rsky/Gt(ROSA)26Sor^+ Tg(Cd4-cre)1Cwi/0 Traf3ip3^tm1c(KOMP)Wtsi/Traf3ip3^tm1c(KOMP)Wtsi	involves: C57BL/6 * C57BL/6N * DBA/2	MGI:5795855
cn7	Gt(ROSA)26Sor^tm8(Map2k1*,EGFP)Rsky/? Tg(Dhh-cre)1Mejr/0	involves: C57BL/6 * FVB/N	MGI:5588152

Genotype
MGI:5588145

cn1

• Allelic Composition: Egr2^tm2(cre)Pch/Egr2⁺; Gt(ROSA)26Sor^{tm8(Map2k1*,EGFP)Rsky}/?; Ptpn11^tm1.1Wbm/Ptpn11^tm1.1Wbm
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

hypermyelination ( J:207470 )

• mice show normal myelination at P15, however axons of peripheral nerves are hypermyelinated by P90

Genotype
MGI:4430561

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm8(Map2k1*,EGFP)Rsky}/Gt(ROSA)26Sor^{tm8(Map2k1*,EGFP)Rsky}; Igh-J^tm1Cgn/Igh^tm4Cgn; Tg(Cr2-cre)3Cgn/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

decreased mature B cell number ( J:157297 )

• mice lack mature BCR positive B cells

hematopoietic system

decreased mature B cell number ( J:157297 )

• mice lack mature BCR positive B cells

Genotype
MGI:5588163

cn3

• Allelic Composition: Erbb3^tm3Cbm/Erbb3^tm3Cbm; Gt(ROSA)26Sor^{tm8(Map2k1*,EGFP)Rsky}/?; Tg(Dhh-cre)1Mejr/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * FVB/N

nervous system

decreased myelin sheath thickness ( J:207470 )

• at P15, the myelin of peripheral axons is thinner but axons are hypermyelinated at P90

abnormal myelination ( J:207470 )

• at P15 and P90, 36.7% and 17.8% of axons with diameters >1 um are nonmyelinated, respectively compared to 3.5% and 0.8% in controls

hypermyelination ( J:207470 )

• axons are hypermyelinated at P90

• however, mice show rescue of Schwann cell development and Schwan cells are able to myelinate

Genotype
MGI:5588150

cn4

• Allelic Composition: Gt(ROSA)26Sor^{tm8(Map2k1*,EGFP)Rsky}/?; Ptpn11^tm1.1Wbm/Ptpn11^tm1.1Wbm; Tg(Dhh-cre)1Mejr/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * FVB/N

nervous system

hypermyelination ( J:207470 )

• axons of peripheral nerves are hypermyelinated at P90

• however, mice show normal numbers of Schwann cells

Genotype
MGI:5588113

cn5

• Allelic Composition: Egr2^tm2(cre)Pch/Egr2⁺; Gt(ROSA)26Sor^{tm8(Map2k1*,EGFP)Rsky}/?
• Genetic Background: involves: 129S2/SvPas * C57BL/6

nervous system

abnormal myelin sheath morphology ( J:207470 )

• onion bulb structures are seen indicating myelin degeneration and regeneration

• however, stacking of myelin sheaths in compact myelin is unaffected

• noncompact myelin structures are more abundant

increased myelin sheath thickness ( J:207470 )

• myelin sheaths are thicker at P15 and P30 and myelin continues to grow radially such that the mean myelin area in peripheral nerves at P90 is increased 6-fold

• increase in myelin thickness is particularly pronounced in small-diameter fibers

tomacula ( J:207470 )

• aberrant paranodal myelin (tomaculi) and infolded myelin loops

abnormal sciatic nerve morphology ( J:207470 )

• increase in the diameter of sciatic nerves due to massive hypermyelination and reduced axonal packing

hypermyelination ( J:207470 )

• myelination begins prematurely and mice show continuous myelin growth

• increase in the number of myelinated fibers per field at P0

• myelin abnormalities are seen at P30-P90 but not at P15, indicating that they result from massive hypermylination

• treatment with the mTORC1 inhibitor everolimus dampens myelin growth in the nerves of mutants and completely rescues the decreased axonal packing

abnormal nerve conduction ( J:207470 )

• ratio of compound action potentials in myelinated A and nonmyelinated C fibers (A/C fiber waves) is reduced and there is a reduction in numbers of active mechanoreceptive units after P60 indicating that many myelinated axons are not conducting

increased nerve conduction velocity ( J:207470 )

• D-hair mechanoreceptors show increased conduction velocities at P30, however no differences were seen after P60

• however, conduction velocities of other mechanosensory fibers are unchanged

Genotype
MGI:5795855

cn6

• Allelic Composition: Gt(ROSA)26Sor^{tm8(Map2k1*,EGFP)Rsky}/Gt(ROSA)26Sor⁺; Tg(Cd4-cre)1Cwi/0; Traf3ip3^{tm1c(KOMP)Wtsi}/Traf3ip3^{tm1c(KOMP)Wtsi}
• Genetic Background: involves: C57BL/6 * C57BL/6N * DBA/2
• Cell Lines: EPD0093_2_B04

immune system

immune system phenotype ( J:226431 )

N • constitutively active Map2k1(MEK) rescues thymocyte development restoring single positive thymocyte and splenic T cell populations

Genotype
MGI:5588152

cn7

• Allelic Composition: Gt(ROSA)26Sor^{tm8(Map2k1*,EGFP)Rsky}/?; Tg(Dhh-cre)1Mejr/0
• Genetic Background: involves: C57BL/6 * FVB/N

nervous system

nervous system phenotype ( J:207470 )

N • rarely observe aberrantly myelinated small axons in mutants and the ratio of nonmyelinated to myelinated axons is unchanged