Phenotypes associated with this allele

• Allele Symbol: Fkbpl^{tm1(KOMP)Wtsi}
• Allele Name: targeted mutation 1, Wellcome Trust Sanger Institute
• Allele ID: MGI:4431442
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fkbpl^tm1(KOMP)Wtsi/Fkbpl^tm1(KOMP)Wtsi	involves: C57BL/6N	MGI:5770093
ht2	Fkbpl^tm1(KOMP)Wtsi/Fkbpl^+	involves: C57BL/6N	MGI:5770092

Genotype
MGI:5770093

hm1

• Allelic Composition: Fkbpl^{tm1(KOMP)Wtsi}/Fkbpl^{tm1(KOMP)Wtsi}
• Genetic Background: involves: C57BL/6N
• Cell Lines: EPD0466_1_C01

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:226964 )

• homozygotes die before E8.5; only empty yolk sacs and resorbed embryos are observed at E8.5 and E9.5

Genotype
MGI:5770092

ht2

• Allelic Composition: Fkbpl^{tm1(KOMP)Wtsi}/Fkbpl⁺
• Genetic Background: involves: C57BL/6N
• Cell Lines: EPD0466_1_C01

cardiovascular system

abnormal intersomitic vessel morphology ( J:226964 )

• at E11.5, intersomitic vessel appear locally distended by a cluster of intraluminal cells

• however, subsequent development and overall organ histology are normal

abnormal tumor vascularization ( J:226964 )

• following intradermal implantation of Lewis lung carcinoma (LLC) tumors, heterozygotes show an increase in CD31-stained blood vessels with the endothelium appearing thicker and less organized than in similarly-treated wild-type controls, indicating enhanced tumor angiogenesis

abnormal vascular branching morphogenesis ( J:226964 )

• in an ex vivo aortic ring assay, heterozygous mutant aortae show enhanced sprouting and disordered branching with a ~2-fold increase in average vessel branching and vessel length relative to wild-type aortae

• treatment of the aortic rings with AD-01 (a therapeutic peptide derivative with potent antiangiogenic activity) abrogates the enhanced sprouting seen in mutant aortae

• treatment with VEGF has an additive enhancement effect on vessel sprouting in mutant aortae relative to similarly-treated wild-type aortae

increased angiogenesis ( J:226964 )

• in an ex vivo aortic ring assay, heterozygous mutant aortae show enhanced sprouting of vessels relative to wild-type aortae

• in an in vivo sponge assay, bFGF-treated polyether sponges implanted in mutant mice show increased recruitment of small (40%) and big (50%) vessels, with a significantly higher number (15% to 20%) of endomucin-stained vessels relative to wild-type controls

abnormal blood vessel physiology ( J:226964 )

• in an in vivo sponge assay, bFGF-treated polyether sponges implanted in mutant mice show increased vessel leakage relative to wild-type controls

neoplasm

abnormal tumor vascularization ( J:226964 )

• following intradermal implantation of Lewis lung carcinoma (LLC) tumors, heterozygotes show an increase in CD31-stained blood vessels with the endothelium appearing thicker and less organized than in similarly-treated wild-type controls, indicating enhanced tumor angiogenesis

increased tumor growth/size ( J:226964 )

• following intradermal implantation of LLC tumors, LLC cells grown on the rear dorsum of heterozygous mutant mice show an increased growth rate relative to those in similarly-treated wild-type controls

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:226964 )

• following intradermal implantation of LLC tumors, heterozygotes show a significantly shorter survival (defined as time to reach >600 mm3 in tumor volume) than similarly-treated wild-type controls