All Phenotypes Smpdl3b<tm1a(EUCOMM)Wtsi> MGI Mouse

increased neutrophil cell number ( J:224870 )

• at 6 hours after i.p. injection of LPS (50 ug), homozygotes display significantly higher neutrophil numbers in the peritoneal lavage fluid than similarly treated wild-type controls

abnormal macrophage morphology ( J:224870 )

• bone marrow-derived macrophages (BMDMs) show a decrease in membrane order (i.e. increase in membrane fluidity) relative to wild-type BMDMs

abnormal circulating chemokine level ( J:224870 )

• at 6 hours after i.p. injection of E. coli (1 x 10⁴ cfu), homozygotes display significantly higher serum KC/CXCL1 levels than similarly treated wild-type controls

increased circulating interleukin-12b level ( J:224870 )

• at 6 hours after i.p. injection of E. coli (1 x 10⁴ cfu), homozygotes display significantly higher serum IL-12p40 levels than similarly treated wild-type controls

• in contrast, serum levels of interleukin-10 (IL-10) and interleukin-1 beta (IL-1b) remain unaffected

increased circulating interleukin-6 level ( J:224870 )

• at 6 hours after i.p. injection of LPS (50 ug) or E. coli (1 x 10⁴ cfu), homozygotes display significantly higher serum IL-6 levels than similarly treated wild-type controls

increased circulating tumor necrosis factor level ( J:224870 )

• at 6 hours after i.p. injection of E. coli (1 x 10⁴ cfu), homozygotes display significantly higher serum TNF levels than similarly treated wild-type controls

abnormal chemokine secretion ( J:224870 )

• upon stimulation with TLR agonists (LPS or CpG), BMDMs show significantly higher expression and/or release of KC/CXCL1 than similarly treated wild-type BMDMs

• upon stimulation with LPS, CpG, imiquimod (IMQ), or Pam3Csk4 (P3C4), bone marrow-derived dendritic cells (BMDCs) show significantly higher expression and/or release of KC/CXCL1 than similarly treated wild-type BMDCs

increased susceptibility to endotoxin shock ( J:224870 )

• at 6 hours after i.p. injection of LPS (50 ug), homozygotes contain significantly more immune cells in the peritoneal lavage fluid, due to enhanced influx of neutrophils, and show significantly higher serum IL-6 levels than similarly treated wild-type controls

• however, serum TNF levels are not significantly altered