All Phenotypes Gatad2b<tm1a(EUCOMM)Hmgu> MGI Mouse

mortality/aging ( J:344365 )

N	• mice survive to adulthood

abnormal facial morphology ( J:344365 )

• 85% of mice exhibit increased curvature of the nasion vs 14% of wild-type controls

broad snout ( J:344365 )

• mice exhibit a significantly broader snout than wild-type controls, as measured by the distance between the most lateral 3rd row of vibrissae

• however, no significant difference is observed in head width

behavior/neurological phenotype ( J:344365 )

N	• mice do NOT exhibit spontaneous seizures at any age and show normal behavior in the grip strength test and the hanging test at 1 month of age

impaired contextual conditioning behavior ( J:344365 )

• in a contextual fear conditioning task, mice exhibit significantly less freezing than wild-type controls in contextual testing 24 h after conditioning

impaired cued conditioning behavior ( J:344365 )

• mice exhibit significantly less freezing in cued conditioning during pre-tone and tone

increased exploration in new environment ( J:344365 )

• mice exhibit novel environment-induced hyperactivity (total distance traveled) in the open field test

abnormal spatial reference memory ( J:344365 )

• in the Barnes maze task, 12-wk-old mice perform worse than wild-type controls at finding the target hole across the acquisition phase

increased thigmotaxis ( J:344365 )

• in the open field test, mice spend significantly more time exploring the periphery while avoiding the anxiety-provoking center

abnormal maternal nurturing ( J:344365 )

• dams are not good mothers; litters derived from wild-type males bred with heterozygous females are nursed by wild-type foster females

increased radial glial cell number ( J:344365 )

• at E16.5, the proportion of radial glial cells is significantly increased in the developing cortex

abnormal brain interneuron morphology ( J:344365 )

• at E16.5, proportions of interneurons are consistently reduced in the developing cortex

abnormal corpus callosum morphology ( J:344365 )

• at P0, significantly less callosal axons cross the midline and the number of midline astroglia cells is decreased

• decreased midline crossing of commissural axons is associated with an abnormal arrangement of GFAP+ guidepost glia cells and a widening of the interhemispheric fissure

abnormal stratification in cerebral cortex ( J:344365 )

• embryonic brains exhibit abnormal density of layer-specific neurons in the cortex

• at E16.5, the frequency of cells co-expressing Satb2 and Ctip2 is significantly higher than in wild-type controls

• at E18.5, the proportion of cells expressing Tbr1 in layer V (co-expressing Ctip2) is significantly higher than in wild-type controls

thin cerebral cortex ( J:344365 )

• embryonic brains exhibit reduced cortical thickness

decreased astrocyte number ( J:344365 )

• at E18, all GFAP+ glia, including the glial wedge (GW), indusium griseum glia (IGG) and midline zipper glia (MZG) subtypes, are generally reduced

abnormal embryonic/fetal subventricular zone morphology ( J:344365 )

• at E16.5, the proportion of progenitor-like cells in the subventricular zone is significantly increased in the developing cortex whereas the proportion of ganglionic eminence cells is reduced