Phenotypes associated with this allele

• Allele Symbol: Fgf21^tm1Emf
• Allele Name: targeted mutation 1, Eleftheria Maratos-Flier
• Allele ID: MGI:4436716
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fgf21^tm1Emf/Fgf21^tm1Emf	B6.Cg-Fgf21^tm1Emf	MGI:4436717

Genotype
MGI:4436717

hm1

• Allelic Composition: Fgf21^tm1Emf/Fgf21^tm1Emf
• Genetic Background: B6.Cg-Fgf21^tm1Emf

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

impaired adaptive thermogenesis ( J:181337 )

• cold exposed mice exhibit reduced body temperatures, reduced activity and increased circulating creatine kinase activity levels compared with wild-type mice

abnormal blood homeostasis ( J:157309 )

increased circulating leptin level ( J:157309 )

• in ketogenetic diet fed mice

decreased body temperature ( J:181337 )

• in cold-exposed mice

decreased oxygen consumption ( J:157309 )

• in the chow fed and fasted state, VO2 levels decreased

abnormal glucose homeostasis ( J:157309 )

increased circulating glucose level ( J:157309 )

• in ketogenetic diet fed mice

increased circulating insulin level ( J:157309 )

• in ketogenetic diet fed mice

impaired glucose tolerance ( J:157309 )

• markedly increased glucose excursion in response to an ip glucose load

increased liver glycogen level ( J:157309 )

• in the livers of mice fed a ketogenetic diet

abnormal lipid homeostasis ( J:157309 )

• exhibit abnormal expression of genes regulating lipid homeostasis in adipose tissue

abnormal lipid level ( J:157309 )

increased circulating cholesterol level ( J:157309 )

• in ketogenetic diet fed mice

increased circulating triglyceride level ( J:157309 )

• in ketogenetic diet fed mice

decreased fatty acids level ( J:157309 )

• decrease in the concentration of non-esterfied fatty acids in fasted mice

increased liver triglyceride level ( J:157309 )

• 2 fold increase in mice fed a ketogenetic diet

increased creatine kinase activity ( J:181337 )

• in cold-exposed mice

impaired lipolysis ( J:157309 )

• decreased levels of the liver ketoneogenesis enzyme beta-hydroxybutyrate in the blood of mice fed a ketogenetic diet

adipose tissue

abnormal adipose tissue morphology ( J:157309 )

increased brown adipose tissue amount ( J:157309 )

• when fed a ketogenetic diet for 14 days

increased total body fat amount ( J:157309 )

• at 24 weeks of age

• longitudinal analysis of body composition reveals a marked increase in body fat content

abnormal brown fat cell morphology ( J:157309 )

• an increase in lipid stores in brown adipose tissue

increased white fat cell size ( J:157309 )

• in perigonadal white adipose tissue

increased gonadal fat pad weight ( J:157309 )

• significant elevation of perigonadal white adipose tissue when fed a ketogenetic diet for 14 days

liver/biliary system

increased liver weight ( J:157309 )

• significant elevation of liver weight when fed a ketogenetic diet for 14 days

increased liver glycogen level ( J:157309 )

• in the livers of mice fed a ketogenetic diet

increased liver triglyceride level ( J:157309 )

• 2 fold increase in mice fed a ketogenetic diet

hepatic steatosis ( J:157309 )

• fatty appearance is reflected at a histological level with lipid accumulation encroaching across the hepatic lobule toward the central vein

abnormal liver physiology ( J:157309 )

• increase in liver glycogen in mice fed a ketogenetic diet

growth/size/body

increased lean body mass ( J:157309 )

• at 14 and 24 weeks of age

abnormal body weight ( J:157309 )

• fasting for 24 hours on leads to less weight loss

increased body weight ( J:157309 )

• at 24 weeks of age

• mice placed on a ketogenetic diet for 2 wk gain substantial weight, unlike wild-type mice that lose weight

increased liver weight ( J:157309 )

• significant elevation of liver weight when fed a ketogenetic diet for 14 days

behavior/neurological

abnormal food intake ( J:157309 )

• moderate increase in daily caloric intake of chow, even when corrected for the increased body weight mice on a ketogenetic diet have a greater caloric intake

decreased locomotor activity ( J:157309 , J:181337 )

• mice on a ketogenetic diet have lower physical activity (J:157309)

• in cold-exposed mice (J:181337)

nervous system

abnormal hypothalamus secretion ( J:157309 )

• hypothalamic neuropeptides revealed higher expression of proopiomelanocortin and lower expression of agouti- related peptide