Phenotypes associated with this allele

• Allele Symbol: Kcnu1^tm1Cmsa
• Allele Name: targeted mutation 1, Celia M Santi
• Allele ID: MGI:4437059
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Kcnu1^tm1Cmsa/Kcnu1^tm1Cmsa	Not Specified	MGI:4437060

Genotype
MGI:4437060

hm1

• Allelic Composition: Kcnu1^tm1Cmsa/Kcnu1^tm1Cmsa
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

decreased sperm progressive motility ( J:157522 )

♂ • markedly deficient in progressive motility relative to sperm from wild-type mice

impaired sperm capacitation ( J:157522 )

♂ • after the capacitation incubating period, 70% of mutant sperm show flagellar angularity between the midpiece and the principal piece, compared to only 30% of the wild-type sperm

♂ • many of the mutant sperm have a "hairpin" configuration

♂ • mutant sperm fail to hyperpolarize their membrane potential after capacitation

♂ • depolarization after the in vitro capacitation

male infertility ( J:157522 )

♂ • mating with multiple wild-type females (2 females per male) produce no offspring in a period of approximately 4 months

impaired fertilization ( J:157522 )

• mutant sperm are unable to fertilize the eggs even after removal of the zona pellucida in in vitro fertilization

impaired acrosome reaction ( J:157522 )

♂ • deficient in the acrosome reaction (AR) treated with the calcium ionophore A23187

♂ • mutant AR phenotype is substantially rescued by the inclusion of the potassium ionophore valinomycin

cellular

decreased sperm progressive motility ( J:157522 )

♂ • markedly deficient in progressive motility relative to sperm from wild-type mice