All Phenotypes Tg(Gnrh1-cre)35Awo MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Kiss1r^tm1.1Hjn/Kiss1r^tm1.1Hjn Tg(Gnrh1-cre)35Awo/0	involves: 129 * C57BL/6 * CD-1	MGI:5660828
cn2	Jak2^tm1Kuw/Jak2^tm1Kuw Tg(Gnrh1-cre)35Awo/0	involves: 129X1/SvJ * CD-1	MGI:6856578
tg3	Tg(Gnrh1-cre)35Awo/0	Not Specified	MGI:4442244

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

oligozoospermia ( J:212351 )

• males display decreased numbers of sperm in the seminiferous tubules and epididymis

abnormal oogenesis ( J:212351 )

• females exhibit disrupted oogenesis, with the presence of primary follicles, preantral follicles, and antral follicles but no evidence of corpora lutea

abnormal anogenital distance ( J:212351 )

• males exhibit a decreased anogenital distance relative to controls

absent corpus luteum ( J:212351 )

• females show no evidence of corpora lutea formation

absent mature ovarian follicles ( J:212351 )

• females do not contain follicles past the antral stage

small ovary ( J:212351 )

decreased ovary weight ( J:212351 )

• females show a ~70% reduction in ovarian weight relative to controls

decreased testis weight ( J:212351 )

• males show a ~70% reduction in testis weight relative to controls

small penis ( J:212351 )

• males exhibit microphallus

small uterus ( J:212351 )

• females display a decrease in uterine size relative to controls

absent sexual maturation ( J:212351 )

• 80% of males fail to exhibit preputial gland separation, unlike control males

delayed sexual maturation ( J:212351 )

• pubertal onset is delayed in both sexes

• however, no differences in body weight are observed relative to controls

delayed vaginal opening ( J:212351 )

• in females, vaginal opening is significantly delayed by ~9 days relative controls

• however, no external anatomic abnormalities are observed

delayed balanopreputial separation ( J:212351 )

• 20% of males show a delay of 7 to 11 days in preputial separation relative to controls

abnormal estrous cycle ( J:212351 )

• females display an extensively abnormal pattern of estrous cyclicity relative to controls

• females were found in metestrus/diestrus 83% of the time, with occasional evidence of proestrus (4%)

• 1 of 6 females was found in proestrus 67% of the time

female infertility ( J:212351 )

male infertility ( J:212351 )

homeostasis/metabolism

abnormal follicle stimulating hormone level ( J:212351 )

• ovariectomized females fail to exhibit a significant increase in serum FSH levels relative to intact females, unlike similarly-treated female controls

decreased circulating follicle stimulating hormone level ( J:212351 )

• basal serum FSH levels are significantly lower in both sexes relative to controls

abnormal luteinizing hormone level ( J:212351 , J:212351 )

	• following exogenous kisspeptin stimulation, serum LH values remain unchanged in both sexes, unlike in similarly-treated controls (J:212351)
	• although gonadotroph function is preserved in both sexes, females, but not males, show a 2-fold increase in GnRH-induced LH serum levels relative to similarly-treated female controls (J:212351) • ovariectomized females fail to exhibit an increase in serum LH levels relative to intact females, unlike similarly-treated female controls (J:212351)

decreased circulating luteinizing hormone level ( J:212351 )

• basal serum LH values are significantly lower in both sexes relative to controls

endocrine/exocrine glands

absent corpus luteum ( J:212351 )

• females show no evidence of corpora lutea formation

absent mature ovarian follicles ( J:212351 )

• females do not contain follicles past the antral stage

small ovary ( J:212351 )

decreased ovary weight ( J:212351 )

• females show a ~70% reduction in ovarian weight relative to controls

decreased testis weight ( J:212351 )

• males show a ~70% reduction in testis weight relative to controls

digestive/alimentary system

abnormal anogenital distance ( J:212351 )

• males exhibit a decreased anogenital distance relative to controls

renal/urinary system

small penis ( J:212351 )

• males exhibit microphallus

cellular

oligozoospermia ( J:212351 )

• males display decreased numbers of sperm in the seminiferous tubules and epididymis

abnormal oogenesis ( J:212351 )

• females exhibit disrupted oogenesis, with the presence of primary follicles, preantral follicles, and antral follicles but no evidence of corpora lutea

Allelic Composition	Jak2^tm1Kuw/Jak2^tm1Kuw Tg(Gnrh1-cre)35Awo/0
Genetic Background	involves: 129X1/SvJ * CD-1

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Jak2^tm1Kuw mutation (1 available); any Jak2 mutation (59 available)
Tg(Gnrh1-cre)35Awo mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

decreased corpora lutea number ( J:167849 )

• number of corpora lutea is severely decreased at 4 months of age

decreased ovary weight ( J:167849 )

• ovary weight is significantly decreased at 4 months of age

delayed female fertility ( J:167849 )

• when mated with wild-type male mice for 90 days, females bear their first litter significantly later than control females

delayed sexual maturation ( J:167849 )

• females display delayed puberty onset: both the time of vaginal opening and the time of first estrus (defined as 100% cornified cells) are significantly delayed

delayed vaginal opening ( J:167849 )

• time of vaginal opening is significantly delayed

decreased ovulation rate ( J:167849 )

abnormal estrous cycle ( J:167849 )

• during a 15-day analysis, only 50% of females exhibit one complete cycle versus 95% in control females; furthermore, only 25% exhibit two complete cycles versus 82% in control females

• while females frequently show persistent diestrus, a number of females go through proestrus

delayed estrous cycle ( J:167849 )

• first estrus (defined as 100% cornified cells) is significantly delayed

prolonged diestrus ( J:167849 )

• acyclic females remain predominantly in persistent metestrus/diestrus

prolonged metestrus ( J:167849 )

• acyclic females remain predominantly in persistent metestrus/diestrus

short proestrus ( J:167849 )

• time spent in the proestrus phase is significantly shortened

reduced female fertility ( J:167849 )

• when mated with wild-type male mice for 90 days, none of females (0 of 8) are able to produce 4 litters whereas 4 of 6 control females do; total number of litters per female is only 2.5 +/- 0.33 versus 3.67 +/- 0.21 in controls

decreased litter size ( J:167849 )

• during 90 days of mating, females produce a significantly lower number of pups per litter relative to control females (5.6 +/- 0.6 versus 12.0 +/- 1.76)

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:167849 )

N	• females show normal basal serum follicle stimulating hormone (FSH) levels; no differences in serum FSH levels are noted after GnRH stimulation or after ovariectomy relative to controls

decreased circulating luteinizing hormone level ( J:167849 )

• females have significantly lower basal serum LH levels than controls; however, serum LH levels are relatively normal after GnRH stimulation or after ovariectomy

• in an LH surge induction paradigm, LH surge levels are significantly blunted relative to controls

• in cycling females, proestrus LH levels are >6-fold lower than those in controls

nervous system

nervous system phenotype ( J:167849 )

N	• mice show no differences in total gonadotropin-releasing hormone (GnRH) neuron number or GnRH cell body distribution across the hypothalamus relative to controls

abnormal neuron physiology ( J:167849 )

• GnRH neuronal activation is significantly attenuated during LH surge induction, as assessed by c-Fos labeling

• however, activation of GnRH neurons after release from estrogen-negative feedback is retained

endocrine/exocrine glands

decreased corpora lutea number ( J:167849 )

• number of corpora lutea is severely decreased at 4 months of age

decreased ovary weight ( J:167849 )

• ovary weight is significantly decreased at 4 months of age

Allelic Composition	Tg(Gnrh1-cre)35Awo/0
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

reproductive system phenotype ( J:157600 )

N	• mice can breed for several generations and show normal reproductive function (fertility)

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