All Phenotypes Tg(Gnrh1-cre)35Awo MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Kiss1r^tm1.1Hjn/Kiss1r^tm1.1Hjn Tg(Gnrh1-cre)35Awo/0	involves: 129 * C57BL/6 * CD-1	MGI:5660828
cn2	Jak2^tm1Kuw/Jak2^tm1Kuw Tg(Gnrh1-cre)35Awo/0	involves: 129X1/SvJ * CD-1	MGI:6856578
tg3	Tg(Gnrh1-cre)35Awo/0	Not Specified	MGI:4442244

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

oligozoospermia ( J:212351 )

• males display decreased numbers of sperm in the seminiferous tubules and epididymis

abnormal oogenesis ( J:212351 )

• females exhibit disrupted oogenesis, with the presence of primary follicles, preantral follicles, and antral follicles but no evidence of corpora lutea

abnormal anogenital distance ( J:212351 )

• males exhibit a decreased anogenital distance relative to controls

absent corpus luteum ( J:212351 )

• females show no evidence of corpora lutea formation

absent mature ovarian follicles ( J:212351 )

• females do not contain follicles past the antral stage

small ovary ( J:212351 )

decreased ovary weight ( J:212351 )

• females show a ~70% reduction in ovarian weight relative to controls

decreased testis weight ( J:212351 )

• males show a ~70% reduction in testis weight relative to controls

small penis ( J:212351 )

• males exhibit microphallus

small uterus ( J:212351 )

• females display a decrease in uterine size relative to controls

absent sexual maturation ( J:212351 )

• 80% of males fail to exhibit preputial gland separation, unlike control males

delayed sexual maturation ( J:212351 )

• pubertal onset is delayed in both sexes

• however, no differences in body weight are observed relative to controls

delayed vaginal opening ( J:212351 )

• in females, vaginal opening is significantly delayed by ~9 days relative controls

• however, no external anatomic abnormalities are observed

delayed balanopreputial separation ( J:212351 )

• 20% of males show a delay of 7 to 11 days in preputial separation relative to controls

abnormal estrous cycle ( J:212351 )

• females display an extensively abnormal pattern of estrous cyclicity relative to controls

• females were found in metestrus/diestrus 83% of the time, with occasional evidence of proestrus (4%)

• 1 of 6 females was found in proestrus 67% of the time

female infertility ( J:212351 )

male infertility ( J:212351 )

homeostasis/metabolism

abnormal follicle stimulating hormone level ( J:212351 )

• ovariectomized females fail to exhibit a significant increase in serum FSH levels relative to intact females, unlike similarly-treated female controls

decreased circulating follicle stimulating hormone level ( J:212351 )

• basal serum FSH levels are significantly lower in both sexes relative to controls

abnormal luteinizing hormone level ( J:212351 , J:212351 )

	• following exogenous kisspeptin stimulation, serum LH values remain unchanged in both sexes, unlike in similarly-treated controls (J:212351)
	• although gonadotroph function is preserved in both sexes, females, but not males, show a 2-fold increase in GnRH-induced LH serum levels relative to similarly-treated female controls (J:212351) • ovariectomized females fail to exhibit an increase in serum LH levels relative to intact females, unlike similarly-treated female controls (J:212351)

decreased circulating luteinizing hormone level ( J:212351 )

• basal serum LH values are significantly lower in both sexes relative to controls

endocrine/exocrine glands

absent corpus luteum ( J:212351 )

• females show no evidence of corpora lutea formation

absent mature ovarian follicles ( J:212351 )

• females do not contain follicles past the antral stage

small ovary ( J:212351 )

decreased ovary weight ( J:212351 )

• females show a ~70% reduction in ovarian weight relative to controls

decreased testis weight ( J:212351 )

• males show a ~70% reduction in testis weight relative to controls

digestive/alimentary system

abnormal anogenital distance ( J:212351 )

• males exhibit a decreased anogenital distance relative to controls

renal/urinary system

small penis ( J:212351 )

• males exhibit microphallus

cellular

oligozoospermia ( J:212351 )

• males display decreased numbers of sperm in the seminiferous tubules and epididymis

abnormal oogenesis ( J:212351 )

• females exhibit disrupted oogenesis, with the presence of primary follicles, preantral follicles, and antral follicles but no evidence of corpora lutea

Allelic Composition	Jak2^tm1Kuw/Jak2^tm1Kuw Tg(Gnrh1-cre)35Awo/0
Genetic Background	involves: 129X1/SvJ * CD-1

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Jak2^tm1Kuw mutation (1 available); any Jak2 mutation (57 available)
Tg(Gnrh1-cre)35Awo mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

decreased corpora lutea number ( J:167849 )

• number of corpora lutea is severely decreased at 4 months of age

decreased ovary weight ( J:167849 )

• ovary weight is significantly decreased at 4 months of age

delayed female fertility ( J:167849 )

• when mated with wild-type male mice for 90 days, females bear their first litter significantly later than control females

delayed sexual maturation ( J:167849 )

• females display delayed puberty onset: both the time of vaginal opening and the time of first estrus (defined as 100% cornified cells) are significantly delayed

delayed vaginal opening ( J:167849 )

• time of vaginal opening is significantly delayed

decreased ovulation rate ( J:167849 )

abnormal estrous cycle ( J:167849 )

• during a 15-day analysis, only 50% of females exhibit one complete cycle versus 95% in control females; furthermore, only 25% exhibit two complete cycles versus 82% in control females

• while females frequently show persistent diestrus, a number of females go through proestrus

delayed estrous cycle ( J:167849 )

• first estrus (defined as 100% cornified cells) is significantly delayed

prolonged diestrus ( J:167849 )

• acyclic females remain predominantly in persistent metestrus/diestrus

prolonged metestrus ( J:167849 )

• acyclic females remain predominantly in persistent metestrus/diestrus

short proestrus ( J:167849 )

• time spent in the proestrus phase is significantly shortened

reduced female fertility ( J:167849 )

• when mated with wild-type male mice for 90 days, none of females (0 of 8) are able to produce 4 litters whereas 4 of 6 control females do; total number of litters per female is only 2.5 +/- 0.33 versus 3.67 +/- 0.21 in controls

decreased litter size ( J:167849 )

• during 90 days of mating, females produce a significantly lower number of pups per litter relative to control females (5.6 +/- 0.6 versus 12.0 +/- 1.76)

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:167849 )

N	• females show normal basal serum follicle stimulating hormone (FSH) levels; no differences in serum FSH levels are noted after GnRH stimulation or after ovariectomy relative to controls

decreased circulating luteinizing hormone level ( J:167849 )

• females have significantly lower basal serum LH levels than controls; however, serum LH levels are relatively normal after GnRH stimulation or after ovariectomy

• in an LH surge induction paradigm, LH surge levels are significantly blunted relative to controls

• in cycling females, proestrus LH levels are >6-fold lower than those in controls

nervous system

nervous system phenotype ( J:167849 )

N	• mice show no differences in total gonadotropin-releasing hormone (GnRH) neuron number or GnRH cell body distribution across the hypothalamus relative to controls

abnormal neuron physiology ( J:167849 )

• GnRH neuronal activation is significantly attenuated during LH surge induction, as assessed by c-Fos labeling

• however, activation of GnRH neurons after release from estrogen-negative feedback is retained

endocrine/exocrine glands

decreased corpora lutea number ( J:167849 )

• number of corpora lutea is severely decreased at 4 months of age

decreased ovary weight ( J:167849 )

• ovary weight is significantly decreased at 4 months of age

Allelic Composition	Tg(Gnrh1-cre)35Awo/0
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

reproductive system phenotype ( J:157600 )

N	• mice can breed for several generations and show normal reproductive function (fertility)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 08/02/2024 MGI 6.24