Phenotypes associated with this allele

• Allele Symbol: Bclaf1^tm1Raz
• Allele Name: targeted mutation 1, Razqallah Hakem
• Allele ID: MGI:4438380
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Bclaf1^tm1Raz/Bclaf1^tm1Raz	involves: 129P2/OlaHsd * C57BL/6	MGI:4438381
ht2	Bclaf1^tm1Raz/Bclaf1^+	involves: 129P2/OlaHsd * C57BL/6	MGI:4438382

Genotype
MGI:4438381

hm1

• Allelic Composition: Bclaf1^tm1Raz/Bclaf1^tm1Raz
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:158087 )

• 95% of mice die between 24 and 48 hours with remaining mice dying at 3 weeks of age

respiratory system

abnormal lung development ( J:158087 )

• at P1, mice exhibit arrested development of the terminal air sacs with mesenchymal thickening unlike in wild-type mice

• however, lung development at E15.5 to E18.5 is normal

abnormal mesenchymal cell differentiation involved in lung development ( J:158087 )

• smooth muscle alpha-actin staining indicates defects in pulmonary mesenchymal differentiation compared to in wild-type mice

thick lung-associated mesenchyme ( J:158087 )

• at P1

immune system

decreased T cell proliferation ( J:158087 )

• peripheral T cells fail to proliferate in response to anti-CD3 and anti-CD28 or anti-CD3 and IL2 unlike similarly treated wild-type cells

increased B cell number ( J:158087 )

• in a Rag2 null reconstitution experiment, B220+ cells are increased compared to in mice reconstituted with wild-type E14.5 fetal liver cells

decreased CD8-positive, alpha-beta T cell number ( J:158087 )

• in a Rag2 null reconstitution experiment, CD4-CD8+ T cells are reduced compared to in mice reconstituted with wild-type E14.5 fetal liver cells

limbs/digits/tail

polydactyly ( J:158087 )

• in 50% of mice on the right hindpaw

growth/size/body

decreased body size ( J:158087 )

behavior/neurological

absent gastric milk in neonates ( J:158087 )

hematopoietic system

decreased T cell proliferation ( J:158087 )

• peripheral T cells fail to proliferate in response to anti-CD3 and anti-CD28 or anti-CD3 and IL2 unlike similarly treated wild-type cells

increased B cell number ( J:158087 )

• in a Rag2 null reconstitution experiment, B220+ cells are increased compared to in mice reconstituted with wild-type E14.5 fetal liver cells

decreased CD8-positive, alpha-beta T cell number ( J:158087 )

• in a Rag2 null reconstitution experiment, CD4-CD8+ T cells are reduced compared to in mice reconstituted with wild-type E14.5 fetal liver cells

cellular

abnormal mesenchymal cell differentiation involved in lung development ( J:158087 )

• smooth muscle alpha-actin staining indicates defects in pulmonary mesenchymal differentiation compared to in wild-type mice

decreased T cell proliferation ( J:158087 )

• peripheral T cells fail to proliferate in response to anti-CD3 and anti-CD28 or anti-CD3 and IL2 unlike similarly treated wild-type cells

Genotype
MGI:4438382

ht2

• Allelic Composition: Bclaf1^tm1Raz/Bclaf1⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

decreased T cell proliferation ( J:158087 )

• peripheral T cells exhibit reduced proliferation in response to anti-CD3 and anti-CD28 or anti-CD3 and IL2 unlike similarly treated wild-type cells

hematopoietic system

decreased T cell proliferation ( J:158087 )

• peripheral T cells exhibit reduced proliferation in response to anti-CD3 and anti-CD28 or anti-CD3 and IL2 unlike similarly treated wild-type cells

cellular

decreased T cell proliferation ( J:158087 )

• peripheral T cells exhibit reduced proliferation in response to anti-CD3 and anti-CD28 or anti-CD3 and IL2 unlike similarly treated wild-type cells