Phenotypes associated with this allele

• Allele Symbol: Bhlhe22^{tm3.1(cre)Meg}
• Allele Name: targeted mutation 3.1, Michael E Greenberg
• Allele ID: MGI:4440745
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Bhlhe22^tm3.1(cre)Meg/Bhlhe22^+ Gata3^tm1Jfz/Gata3^tm2Gsv Gt(ROSA)26Sor^tm1(CAG-Mafb,-tdTomato)Good/Gt(ROSA)26Sor^+	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ	MGI:5581690
cn2	Bhlhe22^tm3.1(cre)Meg/Bhlhe22^+ Gata3^tm1Jfz/Gata3^tm1Jfz	involves: 129S1/Sv * 129X1/SvJ	MGI:5581689
cn3	Bhlhe22^tm3.1(cre)Meg/Bhlhe22^tm1Meg Tg(CAG-Bgeo/GFP)21Lbe/0	involves: 129S1/Sv * 129X1/SvJ	MGI:4440936
cn4	Bhlhe22^tm3.1(cre)Meg/Bhlhe22^tm1Meg Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor^+	involves: 129/Sv * 129S4/SvJaeSor	MGI:4440937
cn5	Bhlhe22^tm3.1(cre)Meg/Bhlhe22^tm1Meg Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor^+	involves: 129/Sv * 129X1/SvJ	MGI:4440935

Genotype
MGI:5581690

cn1

• Allelic Composition: Bhlhe22^{tm3.1(cre)Meg}/Bhlhe22⁺; Gata3^tm1Jfz/Gata3^tm2Gsv; Gt(ROSA)26Sor^{tm1(CAG-Mafb,-tdTomato)Good}/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal inner hair cell synaptic ribbon morphology ( J:207904 )

• partial rescue of ribbon loss observed Mafb^tm1.1Good/Mafb^tm1Jeng Tg(Neurog1-cre)1Jejo mice

abnormal cochlear ganglion morphology ( J:207904 )

• partial rescue of synaptic defects observed Mafb^tm1.1Good/Mafb^tm1Jeng Tg(Neurog1-cre)1Jejo mice

hearing/vestibular/ear

abnormal inner hair cell synaptic ribbon morphology ( J:207904 )

• partial rescue of ribbon loss observed Mafb^tm1.1Good/Mafb^tm1Jeng Tg(Neurog1-cre)1Jejo mice

Genotype
MGI:5581689

cn2

• Allelic Composition: Bhlhe22^{tm3.1(cre)Meg}/Bhlhe22⁺; Gata3^tm1Jfz/Gata3^tm1Jfz
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

nervous system

abnormal cochlear ganglion morphology ( J:207904 )

• cochlear synaptic loss is more severe than in Mafb^tm1.1Good/Mafb^tm1Jeng Tg(Neurog1-cre)1Jejo mice

Genotype
MGI:4440936

cn3

• Allelic Composition: Bhlhe22^{tm3.1(cre)Meg}/Bhlhe22^tm1Meg; Tg(CAG-Bgeo/GFP)21Lbe/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

nervous system

decreased neuron number ( J:158273 )

abnormal spinal cord dorsal horn morphology ( J:158273 )

• in the superficial laminae of spinal cord, there are significantly fewer lacZ-marked neurons compared to controls; significant loss of cell bodies and neuropil is observed

Genotype
MGI:4440937

cn4

• Allelic Composition: Bhlhe22^{tm3.1(cre)Meg}/Bhlhe22^tm1Meg; Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129/Sv * 129S4/SvJaeSor

nervous system

decreased neuron number ( J:158273 )

abnormal spinal cord dorsal horn morphology ( J:158273 )

• in the superficial dorsal horn, there are significantly fewer ( about 50%) lacZ-marked neurons compared to controls

Genotype
MGI:4440935

cn5

• Allelic Composition: Bhlhe22^{tm3.1(cre)Meg}/Bhlhe22^tm1Meg; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129/Sv * 129X1/SvJ

nervous system

abnormal spinal cord dorsal horn morphology ( J:158273 )

• quantification of neurons at P0 shows a 50% decrease in number of marked neurons in superficial dorsal horn compared to controls with no difference in numbers in any other spinal cord region

• significant increase in number of apoptotic cells (marked neurons) in superficial dorsal horn is observed at E18.5, but no difference is observed at E17.5 or 19.5

• partial loss of glutamatergic and GABAergic neurons is observed in dorsal horn

• number of marked neurons in dorsal horn are not different from controls when BrdU labeling is done at E12.5 or E13.5 and analysis is done at E17.5, indicating that neuronal mitosis is not affected