Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^{tm1(CAG-Trp53*,-EGFP)Medz}
• Allele Name: targeted mutation 1, Ruslan Medzhltov
• Allele ID: MGI:4443069
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(CAG-Trp53*,-EGFP)Medz/Gt(ROSA)26Sor^tm1(cre/ERT)Nat	involves: 129 * C57BL/6	MGI:4443107
cn2	Gt(ROSA)26Sor^tm1(CAG-Trp53*,-EGFP)Medz/Gt(ROSA)26Sor^tm1(cre/ERT)Nat Trp53^tm1Tyj/Trp53^+	involves: 129 * 129S2/SvPas * C57BL/6	MGI:4443108
cn3	Gt(ROSA)26Sor^tm1(CAG-Trp53*,-EGFP)Medz/Gt(ROSA)26Sor^tm1(cre/ERT)Nat Trp53^tm1Tyj/Trp53^tm1Tyj	involves: 129 * 129S2/SvPas * C57BL/6	MGI:4443109
cn4	Gt(ROSA)26Sor^tm1(CAG-Trp53*,-EGFP)Medz/Gt(ROSA)26Sor^tm1(cre/ERT)Nat Tg(H2-K-BCL2)1Josd/0	involves: 129 * C3H * C57BL/6	MGI:4443111
cn5	Gt(ROSA)26Sor^tm1(CAG-Trp53*,-EGFP)Medz/Gt(ROSA)26Sor^+ Tg(Nes-cre)1Wme/0	involves: C57BL/6 * CBA	MGI:4443110
cn6	Gt(ROSA)26Sor^tm1(CAG-Trp53*,-EGFP)Medz/Gt(ROSA)26Sor^+ Tg(Mx1-cre)1Cgn/0	involves: C57BL/6 * CBA	MGI:4443118

Genotype
MGI:4443107

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm1(CAG-Trp53*,-EGFP)Medz}/Gt(ROSA)26Sor^{tm1(cre/ERT)Nat}
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:158953 )

N • hematopoietic stem and progenitor cells (HSPCs) of mice treated with tamoxifen do not exhibit a selective advantage over cells not expressing the modified cDNA

N • the total number of HSPCs is normal in irradiated mice

abnormal hematopoietic system physiology ( J:158953 )

• following irradiation, the proportion of GFP+ cells in the hematopoietic stem and progenitor cell compartment and myeloid and lymphoid lineages of tamoxifen-treated mice is increased compared to un-irradiated mice

• GFP+ cell accumulation occurs whether mice are treated with tamoxifen 2 or 7 days after irradiation

• however, no competitive advantage is observed when mice are treated with tamoxifen and 5-fluorouracil without DNA damage or over similarly treated Cdkn2a^tm2.1Rdp cells in irradiated chimera experiments

Genotype
MGI:4443108

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm1(CAG-Trp53*,-EGFP)Medz}/Gt(ROSA)26Sor^{tm1(cre/ERT)Nat}; Trp53^tm1Tyj/Trp53⁺
• Genetic Background: involves: 129 * 129S2/SvPas * C57BL/6

hematopoietic system

hematopoietic system phenotype ( J:158953 )

N • hematopoietic stem and progenitor cells of irradiated mice treated with tamoxifen do not exhibit a selective advantage over cells not expressing the modified cDNA

Genotype
MGI:4443109

cn3

• Allelic Composition: Gt(ROSA)26Sor^{tm1(CAG-Trp53*,-EGFP)Medz}/Gt(ROSA)26Sor^{tm1(cre/ERT)Nat}; Trp53^tm1Tyj/Trp53^tm1Tyj
• Genetic Background: involves: 129 * 129S2/SvPas * C57BL/6

hematopoietic system

hematopoietic system phenotype ( J:158953 )

N • hematopoietic stem and progenitor cells of irradiated mice treated with tamoxifen do not exhibit a selective advantage over cells not expressing the modified cDNA

Genotype
MGI:4443111

cn4

• Allelic Composition: Gt(ROSA)26Sor^{tm1(CAG-Trp53*,-EGFP)Medz}/Gt(ROSA)26Sor^{tm1(cre/ERT)Nat}; Tg(H2-K-BCL2)1Josd/0
• Genetic Background: involves: 129 * C3H * C57BL/6

hematopoietic system

abnormal hematopoietic system physiology ( J:158953 )

• GFP+ hematopoietic stem and progenitor cells (HSPC) from irradiated mice transplanted into irradiated mice reconstitute the HSPC population exhibit a competitive advantage over HSPC from untreated mice compared with cells from similarly irradiated wild-type mice

Genotype
MGI:4443110

cn5

• Allelic Composition: Gt(ROSA)26Sor^{tm1(CAG-Trp53*,-EGFP)Medz}/Gt(ROSA)26Sor⁺; Tg(Nes-cre)1Wme/0
• Genetic Background: involves: C57BL/6 * CBA

hematopoietic system

hematopoietic system phenotype ( J:158953 )

N • hematopoietic stem and progenitor cells of irradiated mice treated with tamoxifen do not exhibit a selective advantage over cells not expressing the modified cDNA

abnormal hematopoietic system physiology ( J:158953 )

• following irradiation, the proportion of GFP+ cells in the hematopoietic stem and progenitor cell compartment and myeloid and lymphoid lineages of tamoxifen-treated mice is increased compared to un-irradiated mice

Genotype
MGI:4443118

cn6

• Allelic Composition: Gt(ROSA)26Sor^{tm1(CAG-Trp53*,-EGFP)Medz}/Gt(ROSA)26Sor⁺; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: C57BL/6 * CBA

hematopoietic system

abnormal hematopoietic system physiology ( J:158953 )

• the percent GFP+ cells in irradiated tamoxifen-treated mice reconstituted with irradiated lymphocyte and HSPC compartments is greater than in mice reconstituted with only the lymphocyte compartment