Phenotypes associated with this allele

• Allele Symbol: Hhipl1^{tm1a(KOMP)Wtsi}
• Allele Name: targeted mutation 1a, Wellcome Trust Sanger Institute
• Allele ID: MGI:4451243
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hhipl1^tm1a(KOMP)Wtsi/Hhipl1^tm1a(KOMP)Wtsi	B6J.B6N-Hhipl1^tm1a(KOMP)Wtsi	MGI:6477557
cx2	Apoe^tm1Unc/Apoe^tm1Unc Hhipl1^tm1a(KOMP)Wtsi/Hhipl1^tm1a(KOMP)Wtsi	involves: 129P2/OlaHsd * C57BL/6J * C57BL/6N	MGI:6477559
cx3	Hhipl1^tm1a(KOMP)Wtsi/Hhipl1^tm1a(KOMP)Wtsi Ldlr^tm1Her/Ldlr^tm1Her	involves: 129S7/SvEvBrd * C57BL/6J * C57BL/6N	MGI:6477558

Genotype
MGI:6477557

hm1

• Allelic Composition: Hhipl1^{tm1a(KOMP)Wtsi}/Hhipl1^{tm1a(KOMP)Wtsi}
• Genetic Background: B6J.B6N-Hhipl1^{tm1a(KOMP)Wtsi}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:280707 )

N • mice are born at normal Mendelian frequencies and show no perinatal or postnatal lethality

growth/size/body

growth/size/body region phenotype ( J:280707 )

N • mice show no signs of dysmorphology or differences in body weight relative to wild-type controls

cardiovascular system

abnormal vascular smooth muscle physiology ( J:280707 )

• in vitro, aortic smooth muscle cells (AoSMCs) display significantly reduced migration relative to wild-type AoSMCs

decreased vascular smooth muscle cell proliferation ( J:280707 )

• in vitro, AoSMCs show significantly reduced proliferation relative to wild-type AoSMCs

• however, no differences in apoptosis are observed

muscle

abnormal vascular smooth muscle physiology ( J:280707 )

• in vitro, aortic smooth muscle cells (AoSMCs) display significantly reduced migration relative to wild-type AoSMCs

decreased vascular smooth muscle cell proliferation ( J:280707 )

• in vitro, AoSMCs show significantly reduced proliferation relative to wild-type AoSMCs

• however, no differences in apoptosis are observed

cellular

decreased vascular smooth muscle cell proliferation ( J:280707 )

• in vitro, AoSMCs show significantly reduced proliferation relative to wild-type AoSMCs

• however, no differences in apoptosis are observed

decreased cell migration ( J:280707 )

• in a scratch wound assay, the migration rate of AoSMCs is significantly lower than that of wild-type AoSMCs

Genotype
MGI:6477559

cx2

• Allelic Composition: Apoe^tm1Unc/Apoe^tm1Unc; Hhipl1^{tm1a(KOMP)Wtsi}/Hhipl1^{tm1a(KOMP)Wtsi}
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J * C57BL/6N

cardiovascular system

decreased susceptibility to atherosclerosis ( J:280707 )

♂ • after 12 weeks on a high-fat Western diet, male double homozygotes show a 53% reduction in % aortic lesion area by en face analysis and a 33% reduction in mean plaque area in aortic roots relative to single Apoe^tm1Unc homozygotes

♂ • under a Western diet, aortic root lesions of double homozygotes show a 47% reduction in SMA-positive staining indicating reduced smooth muscle cell content as well as a reduction in lipid content but no differences in macrophage or collagen content within plaques

♂ • no differences in body weight, plasma lipid levels or blood pressure are observed relative to single Apoe^tm1Unc homozygotes

Genotype
MGI:6477558

cx3

• Allelic Composition: Hhipl1^{tm1a(KOMP)Wtsi}/Hhipl1^{tm1a(KOMP)Wtsi}; Ldlr^tm1Her/Ldlr^tm1Her
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J * C57BL/6N

cardiovascular system

decreased susceptibility to atherosclerosis ( J:280707 )

♂ • after 12 weeks on a high-fat Western diet, male double homozygotes show a 57% reduction in % aortic lesion area by en face analysis and a 37% reduction in mean plaque area in aortic roots with smaller lipid cores relative to single Ldlr^tm1Her homozygotes

♂ • under a Western diet, aortic root lesions of double homozygotes show a 46% reduction in anti-alpha smooth muscle actin (SMA)-positive staining indicating reduced smooth muscle cell content but no differences in lipid, macrophage or collagen content within plaques

♂ • no differences in body weight, plasma lipid levels or blood pressure are observed relative to single Ldlr^tm1Her homozygotes