Phenotypes associated with this allele

• Allele Symbol: Lacc1^{tm1a(KOMP)Wtsi}
• Allele Name: targeted mutation 1a, Wellcome Trust Sanger Institute
• Allele ID: MGI:4451304
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lacc1^tm1a(KOMP)Wtsi/Lacc1^tm1a(KOMP)Wtsi	involves: C57BL/6N * C57BL/6NTac	MGI:6118073

Genotype
MGI:6118073

hm1

• Allelic Composition: Lacc1^{tm1a(KOMP)Wtsi}/Lacc1^{tm1a(KOMP)Wtsi}
• Genetic Background: involves: C57BL/6N * C57BL/6NTac
• Cell Lines: EPD0538_1_D06

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal macrophage morphology ( J:244365 )

• reduced abundance of total long-chained saturated fatty acid-CoA

• however, total fatty-acid content is normal

abnormal macrophage physiology ( J:244365 )

• macrophages exhibit a reduction in glycolysis and basal or maximal uncontrolled oxygen consumption rate compared with wild-type cells

• M1 and M2 exhibit reduced oxidative capacity compared with wild-type mice

• macrophages exhibit reduced fatty acid oxidation compared with wild-type cells

• reduced total cellular ATP in bone marrow-derived M1 and M2 macrophages

• macrophages exhibit less intracellular killing of a luminescent Mycobacterium bovis bacillus Calmette-Guerin (BCG) strain and Salmonella enterica serovar Typhimurium compared with wild type macrophages

increased circulating interleukin-1 beta level ( J:244365 )

• in LPS-treated mice (10 mg per kg body weight)

decreased circulating tumor necrosis factor level ( J:244365 )

• in LPS-treated mice (2 mg per kg body weight)

decreased interleukin-1 beta secretion ( J:244365 )

• in LPS-treated mice (2 mg per kg body weight)

increased susceptibility to endotoxin shock ( J:244365 )

• mice injected with LPS (10 mg per kg body weight) exhibit higher sepsis scores with two of seven mice exhibiting epistaxis compared with wild-type mice

homeostasis/metabolism

increased circulating interleukin-1 beta level ( J:244365 )

• in LPS-treated mice (10 mg per kg body weight)

decreased circulating tumor necrosis factor level ( J:244365 )

• in LPS-treated mice (2 mg per kg body weight)

decreased fatty acids level ( J:244365 )

• reduced abundance of total long-chained saturated fatty acid-CoA in macrophages

• however, total fatty-acid content is normal

cellular

abnormal cellular respiration ( J:244365 )

• M1 and M2 exhibit reduced oxidative capacity compared with wild-type mice

abnormal oxidative phosphorylation ( J:244365 )

• reduced total cellular ATP in bone marrow-derived M1 and M2 macrophages

abnormal redox activity ( J:244365 )

• reduced mitochondrial and extracellular reactive oxygen species in M1 and M2 macrophages

• reduced cytoplasmic reactive oxygen species in M1, but not M2, macrophages

hematopoietic system

abnormal macrophage morphology ( J:244365 )

• reduced abundance of total long-chained saturated fatty acid-CoA

• however, total fatty-acid content is normal

abnormal macrophage physiology ( J:244365 )

• macrophages exhibit a reduction in glycolysis and basal or maximal uncontrolled oxygen consumption rate compared with wild-type cells

• M1 and M2 exhibit reduced oxidative capacity compared with wild-type mice

• macrophages exhibit reduced fatty acid oxidation compared with wild-type cells

• reduced total cellular ATP in bone marrow-derived M1 and M2 macrophages

• macrophages exhibit less intracellular killing of a luminescent Mycobacterium bovis bacillus Calmette-Guerin (BCG) strain and Salmonella enterica serovar Typhimurium compared with wild type macrophages