Phenotypes associated with this allele

• Allele Symbol: Myl3^{tm1a(EUCOMM)Hmgu}
• Allele Name: targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• Allele ID: MGI:4452746
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Myl3^tm1a(EUCOMM)Hmgu/Myl3^tm1a(EUCOMM)Hmgu Tg(Col2a1-cre)1Xya/0	involves: C57BL/6J * C57BL/6N	MGI:7578779
cn2	Myl3^tm1a(EUCOMM)Hmgu/Myl3^tm1a(EUCOMM)Hmgu Tg(Col2a1-cre/ERT)KA3Smac/0	involves: C57BL/6J * C57BL/6N * FVB/N	MGI:7578782

Genotype
MGI:7578779

cn1

• Allelic Composition: Myl3^{tm1a(EUCOMM)Hmgu}/Myl3^{tm1a(EUCOMM)Hmgu}; Tg(Col2a1-cre)1Xya/0
• Genetic Background: involves: C57BL/6J * C57BL/6N
• Cell Lines: HEPD0622_5_G02

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

osteoarthritis ( J:343166 )

• 6-month-old mice exhibit cartilage destruction which becomes severe at 18 months of age indicating spontaneous age-related osteoarthritis

• cartilage destruction is accompanied by aggravated synovial inflammation but comparable osteophyte maturity as in controls

• expression of senescence, DNA damage, and catabolic markers are decreased in cartilage of aged mice

• however, mice exhibit normal skeletal development, with normal organization of growth plates and normal body weight

skeleton

osteoarthritis ( J:343166 )

• 6-month-old mice exhibit cartilage destruction which becomes severe at 18 months of age indicating spontaneous age-related osteoarthritis

• cartilage destruction is accompanied by aggravated synovial inflammation but comparable osteophyte maturity as in controls

• expression of senescence, DNA damage, and catabolic markers are decreased in cartilage of aged mice

• however, mice exhibit normal skeletal development, with normal organization of growth plates and normal body weight

abnormal chondrocyte physiology ( J:343166 )

• marker analysis indicates enhanced cellular senescence of chondrocytes

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
osteoarthritis		DOID:8398		J:343166

Genotype
MGI:7578782

cn2

• Allelic Composition: Myl3^{tm1a(EUCOMM)Hmgu}/Myl3^{tm1a(EUCOMM)Hmgu}; Tg(Col2a1-cre/ERT)KA3Smac/0
• Genetic Background: involves: C57BL/6J * C57BL/6N * FVB/N
• Cell Lines: HEPD0622_5_G02

immune system

increased susceptibility to induced arthritis ( J:343166 )

♂ • tamoxifen-treated mice undergoing destabilization of the medial meniscus (DMM) surgery-induced osteoarthritis show more severe osteoarthritis progression at both 6- and 10-weeks post DMM surgery, showing reduced cartilage areas, aggravated synovial inflammation, partially increased osteophyte maturity, increased OARSI scores and enhanced cellular senescence of chondrocytes

♂ • the severity of DMM-induced osteoarthritis in tamoxifen-treated mice is comparable to that in controls after dynasore, a CME inhibitor, treatment or after RO4929097, a Notch pathway inhibitor, treatment, with reduced cartilage destruction and diminished numbers of senescent chondrocytes

skeleton

increased susceptibility to induced arthritis ( J:343166 )

♂ • tamoxifen-treated mice undergoing destabilization of the medial meniscus (DMM) surgery-induced osteoarthritis show more severe osteoarthritis progression at both 6- and 10-weeks post DMM surgery, showing reduced cartilage areas, aggravated synovial inflammation, partially increased osteophyte maturity, increased OARSI scores and enhanced cellular senescence of chondrocytes

♂ • the severity of DMM-induced osteoarthritis in tamoxifen-treated mice is comparable to that in controls after dynasore, a CME inhibitor, treatment or after RO4929097, a Notch pathway inhibitor, treatment, with reduced cartilage destruction and diminished numbers of senescent chondrocytes